Detection of enzyme polymorphism by using monoclonal antibodies

Clive A. Slaughter, M. C. Coseo, M. P. Cancro, H. Harris

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Six monoclonal antibodies against human placental alkaline phosphatase [orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1], a highly polymorphic enzyme, were tested for reactivity against a panel of 295 placental extracts that had been typed electrophoretically. The products of the three common alleles as well as several rare alleles could be discriminated by the various antibodies. In some cases differences between allelic products were reflected by essentially 'all-or-none' reactions, but in other cases the differences were smaller and demonstrable only by quantitative analysis of the binding. Evidence for allelic differences not detectable electrophoretically was also obtained.

Original languageEnglish (US)
Pages (from-to)1124-1128
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume78
Issue number2 II
StatePublished - Jan 1 1981
Externally publishedYes

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Placental Extracts
Alleles
Monoclonal Antibodies
Enzymes
Alkaline Phosphatase
Antibodies
placental alkaline phosphatase

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Detection of enzyme polymorphism by using monoclonal antibodies. / Slaughter, Clive A.; Coseo, M. C.; Cancro, M. P.; Harris, H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 78, No. 2 II, 01.01.1981, p. 1124-1128.

Research output: Contribution to journalArticle

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