Abstract
Six monoclonal antibodies against human placental alkaline phosphatase [orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1], a highly polymorphic enzyme, were tested for reactivity against a panel of 295 placental extracts that had been typed electrophoretically. The products of the three common alleles as well as several rare alleles could be discriminated by the various antibodies. In some cases differences between allelic products were reflected by essentially 'all-or-none' reactions, but in other cases the differences were smaller and demonstrable only by quantitative analysis of the binding. Evidence for allelic differences not detectable electrophoretically was also obtained.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1124-1128 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 78 |
| Issue number | 2 II |
| State | Published - Jan 1 1981 |
| Externally published | Yes |
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ASJC Scopus subject areas
- General
- Genetics
Cite this
Detection of enzyme polymorphism by using monoclonal antibodies. / Slaughter, Clive A.; Coseo, M. C.; Cancro, M. P.; Harris, H.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 78, No. 2 II, 01.01.1981, p. 1124-1128.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of enzyme polymorphism by using monoclonal antibodies
AU - Slaughter, Clive A.
AU - Coseo, M. C.
AU - Cancro, M. P.
AU - Harris, H.
PY - 1981/1/1
Y1 - 1981/1/1
N2 - Six monoclonal antibodies against human placental alkaline phosphatase [orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1], a highly polymorphic enzyme, were tested for reactivity against a panel of 295 placental extracts that had been typed electrophoretically. The products of the three common alleles as well as several rare alleles could be discriminated by the various antibodies. In some cases differences between allelic products were reflected by essentially 'all-or-none' reactions, but in other cases the differences were smaller and demonstrable only by quantitative analysis of the binding. Evidence for allelic differences not detectable electrophoretically was also obtained.
AB - Six monoclonal antibodies against human placental alkaline phosphatase [orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1], a highly polymorphic enzyme, were tested for reactivity against a panel of 295 placental extracts that had been typed electrophoretically. The products of the three common alleles as well as several rare alleles could be discriminated by the various antibodies. In some cases differences between allelic products were reflected by essentially 'all-or-none' reactions, but in other cases the differences were smaller and demonstrable only by quantitative analysis of the binding. Evidence for allelic differences not detectable electrophoretically was also obtained.
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UR - http://www.scopus.com/inward/citedby.url?scp=0019427298&partnerID=8YFLogxK
M3 - Article
VL - 78
SP - 1124
EP - 1128
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 2 II
ER -