Detection of human cytomegalovirus genome in uterus tissue

T. Furukawa, F. Jisaki, Daitoku Sakamuro, T. Takegami, T. Murayama

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Abstract

To analyze persistent infection by human cytomegalovirus (HCMV) in vivo, specimens obtained from various sources and autopsied organs were examined for the presence of HCMV DNA, mRNA transcripts and antigens by polymerase chain reaction, in situ hybridization and immunostaining. The HCMV genome was detected in lung, liver, kidney, and blood vessels at an average positive rate of 15%. The highest PCR-positive rate was observed with cervical smears. Subsequent examination of uterus tissues from patients with myoma revealed HCMV transcripts and antigens in glandular epithelial cells, leukocytes, endothelial cells, and others, indicating productive HCMV infection of cervical tissue.

Original languageEnglish (US)
Pages (from-to)265-277
Number of pages13
JournalArchives of Virology
Volume135
Issue number3-4
DOIs
StatePublished - Sep 1 1994
Externally publishedYes

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ASJC Scopus subject areas

  • Virology

Cite this

Furukawa, T., Jisaki, F., Sakamuro, D., Takegami, T., & Murayama, T. (1994). Detection of human cytomegalovirus genome in uterus tissue. Archives of Virology, 135(3-4), 265-277. https://doi.org/10.1007/BF01310013