Detection of human cytomegalovirus genome in uterus tissue

T. Furukawa, F. Jisaki, Daitoku Sakamuro, T. Takegami, T. Murayama

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

To analyze persistent infection by human cytomegalovirus (HCMV) in vivo, specimens obtained from various sources and autopsied organs were examined for the presence of HCMV DNA, mRNA transcripts and antigens by polymerase chain reaction, in situ hybridization and immunostaining. The HCMV genome was detected in lung, liver, kidney, and blood vessels at an average positive rate of 15%. The highest PCR-positive rate was observed with cervical smears. Subsequent examination of uterus tissues from patients with myoma revealed HCMV transcripts and antigens in glandular epithelial cells, leukocytes, endothelial cells, and others, indicating productive HCMV infection of cervical tissue.

Original languageEnglish (US)
Pages (from-to)265-277
Number of pages13
JournalArchives of Virology
Volume135
Issue number3-4
DOIs
StatePublished - Sep 1 1994
Externally publishedYes

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Human Genome
Cytomegalovirus
Uterus
Cytomegalovirus Infections
Antigens
Polymerase Chain Reaction
Myoma
Vaginal Smears
In Situ Hybridization
Blood Vessels
Leukocytes
Endothelial Cells
Epithelial Cells
Kidney
Lung
Messenger RNA
Liver
DNA

ASJC Scopus subject areas

  • Virology

Cite this

Furukawa, T., Jisaki, F., Sakamuro, D., Takegami, T., & Murayama, T. (1994). Detection of human cytomegalovirus genome in uterus tissue. Archives of Virology, 135(3-4), 265-277. https://doi.org/10.1007/BF01310013

Detection of human cytomegalovirus genome in uterus tissue. / Furukawa, T.; Jisaki, F.; Sakamuro, Daitoku; Takegami, T.; Murayama, T.

In: Archives of Virology, Vol. 135, No. 3-4, 01.09.1994, p. 265-277.

Research output: Contribution to journalArticle

Furukawa, T, Jisaki, F, Sakamuro, D, Takegami, T & Murayama, T 1994, 'Detection of human cytomegalovirus genome in uterus tissue', Archives of Virology, vol. 135, no. 3-4, pp. 265-277. https://doi.org/10.1007/BF01310013
Furukawa, T. ; Jisaki, F. ; Sakamuro, Daitoku ; Takegami, T. ; Murayama, T. / Detection of human cytomegalovirus genome in uterus tissue. In: Archives of Virology. 1994 ; Vol. 135, No. 3-4. pp. 265-277.
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