Detection of human papillomavirus DNA in cervical cancer tissue by the polymerase chain reaction

Yukai He; Ji zeng Zhang; Qian Xu; Ji ming Gao

doi:10.1016/0166-0934(89)90070-0

Detection of human papillomavirus DNA in cervical cancer tissue by the polymerase chain reaction

Yukai He, Ji zeng Zhang, Qian Xu, Ji ming Gao

Research output: Contribution to journal › Article

5 Scopus citations

Abstract

A method for detecting HPV DNA in cervical cancer tissue was developed without using isotopes. The DNA samples from the cancer tissues were first subjected to amplification by PCR, followed by polyacrylamide gel electrophoresis to identify the specific amplified fragment. The specificity and sensitivity of the PCR method are described. Compared with the dot hybridization technique, it is shown that the method is able to detect HPV DNA in cervical cancer tissues.

Original language	English (US)
Pages (from-to)	17-25
Number of pages	9
Journal	Journal of Virological Methods
Volume	26
Issue number	1
DOIs	https://doi.org/10.1016/0166-0934(89)90070-0
Publication status	Published - Jan 1 1989
Externally published	Yes

Fingerprint

Keywords

Cervical cancer
DNA amplification in vitro
Human papillomavirus
Polymerase chain reaction

ASJC Scopus subject areas

Virology

Cite this

He, Y., Zhang, J. Z., Xu, Q., & Gao, J. M. (1989). Detection of human papillomavirus DNA in cervical cancer tissue by the polymerase chain reaction. Journal of Virological Methods, 26(1), 17-25. https://doi.org/10.1016/0166-0934(89)90070-0

@article{15a2491c587142f9be3d1fa527b139ec,

title = "Detection of human papillomavirus DNA in cervical cancer tissue by the polymerase chain reaction",

abstract = "A method for detecting HPV DNA in cervical cancer tissue was developed without using isotopes. The DNA samples from the cancer tissues were first subjected to amplification by PCR, followed by polyacrylamide gel electrophoresis to identify the specific amplified fragment. The specificity and sensitivity of the PCR method are described. Compared with the dot hybridization technique, it is shown that the method is able to detect HPV DNA in cervical cancer tissues.",

keywords = "Cervical cancer, DNA amplification in vitro, Human papillomavirus, Polymerase chain reaction",

author = "Yukai He and Zhang, {Ji zeng} and Qian Xu and Gao, {Ji ming}",

year = "1989",

month = "1",

day = "1",

doi = "10.1016/0166-0934(89)90070-0",

language = "English (US)",

volume = "26",

pages = "17--25",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Detection of human papillomavirus DNA in cervical cancer tissue by the polymerase chain reaction

AU - He, Yukai

AU - Zhang, Ji zeng

AU - Xu, Qian

AU - Gao, Ji ming

PY - 1989/1/1

Y1 - 1989/1/1

N2 - A method for detecting HPV DNA in cervical cancer tissue was developed without using isotopes. The DNA samples from the cancer tissues were first subjected to amplification by PCR, followed by polyacrylamide gel electrophoresis to identify the specific amplified fragment. The specificity and sensitivity of the PCR method are described. Compared with the dot hybridization technique, it is shown that the method is able to detect HPV DNA in cervical cancer tissues.

AB - A method for detecting HPV DNA in cervical cancer tissue was developed without using isotopes. The DNA samples from the cancer tissues were first subjected to amplification by PCR, followed by polyacrylamide gel electrophoresis to identify the specific amplified fragment. The specificity and sensitivity of the PCR method are described. Compared with the dot hybridization technique, it is shown that the method is able to detect HPV DNA in cervical cancer tissues.

KW - Cervical cancer

KW - DNA amplification in vitro

KW - Human papillomavirus

KW - Polymerase chain reaction

UR - http://www.scopus.com/inward/record.url?scp=0024422276&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024422276&partnerID=8YFLogxK

U2 - 10.1016/0166-0934(89)90070-0

DO - 10.1016/0166-0934(89)90070-0

M3 - Article

C2 - 2556422

AN - SCOPUS:0024422276

VL - 26

SP - 17

EP - 25

JO - Journal of Virological Methods

JF - Journal of Virological Methods

SN - 0166-0934

IS - 1

ER -

Access to Document

10.1016/0166-0934(89)90070-0