Detection of human papillomavirus DNA in cervical cancer tissue by the polymerase chain reaction

Yukai He, Ji zeng Zhang, Qian Xu, Ji ming Gao

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A method for detecting HPV DNA in cervical cancer tissue was developed without using isotopes. The DNA samples from the cancer tissues were first subjected to amplification by PCR, followed by polyacrylamide gel electrophoresis to identify the specific amplified fragment. The specificity and sensitivity of the PCR method are described. Compared with the dot hybridization technique, it is shown that the method is able to detect HPV DNA in cervical cancer tissues.

Original languageEnglish (US)
Pages (from-to)17-25
Number of pages9
JournalJournal of Virological Methods
Volume26
Issue number1
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Uterine Cervical Neoplasms
Polymerase Chain Reaction
DNA
Isotopes
Polyacrylamide Gel Electrophoresis
Sensitivity and Specificity
Neoplasms

Keywords

  • Cervical cancer
  • DNA amplification in vitro
  • Human papillomavirus
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Virology

Cite this

Detection of human papillomavirus DNA in cervical cancer tissue by the polymerase chain reaction. / He, Yukai; Zhang, Ji zeng; Xu, Qian; Gao, Ji ming.

In: Journal of Virological Methods, Vol. 26, No. 1, 01.01.1989, p. 17-25.

Research output: Contribution to journalArticle

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