Abstract
A method for detecting HPV DNA in cervical cancer tissue was developed without using isotopes. The DNA samples from the cancer tissues were first subjected to amplification by PCR, followed by polyacrylamide gel electrophoresis to identify the specific amplified fragment. The specificity and sensitivity of the PCR method are described. Compared with the dot hybridization technique, it is shown that the method is able to detect HPV DNA in cervical cancer tissues.
Original language | English (US) |
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Pages (from-to) | 17-25 |
Number of pages | 9 |
Journal | Journal of Virological Methods |
Volume | 26 |
Issue number | 1 |
DOIs | |
State | Published - Oct 1989 |
Externally published | Yes |
Keywords
- Cervical cancer
- DNA amplification in vitro
- Human papillomavirus
- Polymerase chain reaction
ASJC Scopus subject areas
- Virology