Abstract
A method for detecting HPV DNA in cervical cancer tissue was developed without using isotopes. The DNA samples from the cancer tissues were first subjected to amplification by PCR, followed by polyacrylamide gel electrophoresis to identify the specific amplified fragment. The specificity and sensitivity of the PCR method are described. Compared with the dot hybridization technique, it is shown that the method is able to detect HPV DNA in cervical cancer tissues.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 17-25 |
| Number of pages | 9 |
| Journal | Journal of Virological Methods |
| Volume | 26 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1989 |
| Externally published | Yes |
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Keywords
- Cervical cancer
- DNA amplification in vitro
- Human papillomavirus
- Polymerase chain reaction
ASJC Scopus subject areas
- Virology
Cite this
Detection of human papillomavirus DNA in cervical cancer tissue by the polymerase chain reaction. / He, Yukai; Zhang, Ji zeng; Xu, Qian; Gao, Ji ming.
In: Journal of Virological Methods, Vol. 26, No. 1, 01.01.1989, p. 17-25.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of human papillomavirus DNA in cervical cancer tissue by the polymerase chain reaction
AU - He, Yukai
AU - Zhang, Ji zeng
AU - Xu, Qian
AU - Gao, Ji ming
PY - 1989/1/1
Y1 - 1989/1/1
N2 - A method for detecting HPV DNA in cervical cancer tissue was developed without using isotopes. The DNA samples from the cancer tissues were first subjected to amplification by PCR, followed by polyacrylamide gel electrophoresis to identify the specific amplified fragment. The specificity and sensitivity of the PCR method are described. Compared with the dot hybridization technique, it is shown that the method is able to detect HPV DNA in cervical cancer tissues.
AB - A method for detecting HPV DNA in cervical cancer tissue was developed without using isotopes. The DNA samples from the cancer tissues were first subjected to amplification by PCR, followed by polyacrylamide gel electrophoresis to identify the specific amplified fragment. The specificity and sensitivity of the PCR method are described. Compared with the dot hybridization technique, it is shown that the method is able to detect HPV DNA in cervical cancer tissues.
KW - Cervical cancer
KW - DNA amplification in vitro
KW - Human papillomavirus
KW - Polymerase chain reaction
UR - http://www.scopus.com/inward/record.url?scp=0024422276&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024422276&partnerID=8YFLogxK
U2 - 10.1016/0166-0934(89)90070-0
DO - 10.1016/0166-0934(89)90070-0
M3 - Article
VL - 26
SP - 17
EP - 25
JO - Journal of Virological Methods
JF - Journal of Virological Methods
SN - 0166-0934
IS - 1
ER -