Detection of inducible nitric oxide synthase in Schistosoma japonicum and S. mansoni

X. C. Long, M. Bahgat, K. Chlichlia, A. Ruppel, Y. L. Li

Research output: Contribution to journalArticle

Abstract

Schistosoma japonicum and S. mansoni were tested for reactivity with an anti-inducible nitric oxide (iNOS) antibody and the distribution of iNOS was studied by immunofluorescent tests in different stages of the parasites. Reactivity was associated with the tegument in both larval schistosomes (sporocysts and cercariae) and eggs. With adult worms, the majority of the immunofluorescence was predominantly subtegumental in S. japonicum and parenchymal in S. mansoni. Fluorescence was also observed in host tissues (snails and mouse liver). In Western blots, the enzyme of S. japonicum had an apparent molecular weight of about 210 kDa. The possible role of worm and host iNOS in the parasite-host interrelation remains to be clarified.

Original languageEnglish (US)
Pages (from-to)47-50
Number of pages4
JournalJournal of Helminthology
Volume78
Issue number1
DOIs
StatePublished - Mar 1 2004
Externally publishedYes

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Schistosoma japonicum
Schistosoma mansoni
Nitric Oxide Synthase Type II
Parasites
parasites
Cercaria
sporocysts (Protozoa)
Schistosoma
Oocysts
cercariae
Snails
Eggs
fluorescent antibody technique
Fluorescent Antibody Technique
nitric oxide
snails
Nitric Oxide
Western blotting
Molecular Weight
Fluorescence

ASJC Scopus subject areas

  • Parasitology
  • Animal Science and Zoology

Cite this

Detection of inducible nitric oxide synthase in Schistosoma japonicum and S. mansoni. / Long, X. C.; Bahgat, M.; Chlichlia, K.; Ruppel, A.; Li, Y. L.

In: Journal of Helminthology, Vol. 78, No. 1, 01.03.2004, p. 47-50.

Research output: Contribution to journalArticle

Long, X. C. ; Bahgat, M. ; Chlichlia, K. ; Ruppel, A. ; Li, Y. L. / Detection of inducible nitric oxide synthase in Schistosoma japonicum and S. mansoni. In: Journal of Helminthology. 2004 ; Vol. 78, No. 1. pp. 47-50.
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