Detection of migration of locally implanted AC133+ stem cells by cellular magnetic resonance imaging with histological findings

Ali Syed Arbab, Branislava Janic, Robert A. Knight, Stasia A. Anderson, Edyta Pawelczyk, Ali M. Rad, Elizabeth J. Read, Sunil D. Pandit, Joseph A. Frank

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

This study investigated the factors responsible for migration and homing of magnetically labeled AC133+ cells at the sites of active angiogenesis in tumor. AC133+ cells labeled with ferumoxide-protamine sulfate were mixed with either rat glioma or human melanoma cells and implanted in flank of nude mice. An MRI of the tumors including surrounding tissues was performed. Tumor sections were stained for Prussian blue (PB), platelet-derived growth factor (PDGF), hypoxia-inducible factor-1α (HIF-1α), stromal cell derived factor-1 (SDF-1), matrix metalloproteinase-2 (MMP-2), vascular endothelial growth factor (VEGF), and endothelial markers. Fresh snap-frozen strips from the central and peripheral parts of the tumor were collected for Western blotting. MRIs demonstrated hypointense regions at the periphery of the tumors where the PB+/AC133+ cells were positive for endothelial cells markers. At the sites of PB+/AC133+ cells, both HIF-1α and SDF-1 were strongly positive and PDGF and MMP-2 showed generalized expression in the tumor and surrounding tissues. There was no significant association of PB+/AC133+ cell localization and VEGF expression in tumor cells. Western blot demonstrated strong expression of the SDF-1, MMP-2, and PDGF at the peripheral parts of the tumors. HIF-1α was expressed at both the periphery and central parts of the tumor. This work demonstrates that magnetically labeled cells can be used as probes for MRI and histological identification of administered cells. - Arbab, A. S., Janic, B., Knight, R. A., Anderson, S. A., Pawelczyk, E., Rad, A. M., Read, E. J., Pandit, S. D., Frank, J. A. Detection of migration of locally implanted AC133+ stem cells by cellular magnetic resonance imaging with histological findings.

Original languageEnglish (US)
Pages (from-to)3234-3246
Number of pages13
JournalFASEB Journal
Volume22
Issue number9
DOIs
StatePublished - Sep 1 2008
Externally publishedYes

Fingerprint

Magnetic resonance
Stem cells
magnetic resonance imaging
stem cells
Tumors
Stem Cells
Magnetic Resonance Imaging
Imaging techniques
neoplasms
platelet-derived growth factor
gelatinase A
Chemokine CXCL12
Hypoxia-Inducible Factor 1
Neoplasms
Matrix Metalloproteinase 2
Platelet-Derived Growth Factor
cells
Magnetic resonance imaging
vascular endothelial growth factors
Vascular Endothelial Growth Factor A

Keywords

  • Angiogenesis
  • MRI
  • Magnetically labeled AC133 cells
  • Tumors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Detection of migration of locally implanted AC133+ stem cells by cellular magnetic resonance imaging with histological findings. / Arbab, Ali Syed; Janic, Branislava; Knight, Robert A.; Anderson, Stasia A.; Pawelczyk, Edyta; Rad, Ali M.; Read, Elizabeth J.; Pandit, Sunil D.; Frank, Joseph A.

In: FASEB Journal, Vol. 22, No. 9, 01.09.2008, p. 3234-3246.

Research output: Contribution to journalArticle

Arbab, AS, Janic, B, Knight, RA, Anderson, SA, Pawelczyk, E, Rad, AM, Read, EJ, Pandit, SD & Frank, JA 2008, 'Detection of migration of locally implanted AC133+ stem cells by cellular magnetic resonance imaging with histological findings', FASEB Journal, vol. 22, no. 9, pp. 3234-3246. https://doi.org/10.1096/fj.07-105676
Arbab, Ali Syed ; Janic, Branislava ; Knight, Robert A. ; Anderson, Stasia A. ; Pawelczyk, Edyta ; Rad, Ali M. ; Read, Elizabeth J. ; Pandit, Sunil D. ; Frank, Joseph A. / Detection of migration of locally implanted AC133+ stem cells by cellular magnetic resonance imaging with histological findings. In: FASEB Journal. 2008 ; Vol. 22, No. 9. pp. 3234-3246.
@article{902e68b8ef114f4898b113fc81f54a58,
title = "Detection of migration of locally implanted AC133+ stem cells by cellular magnetic resonance imaging with histological findings",
abstract = "This study investigated the factors responsible for migration and homing of magnetically labeled AC133+ cells at the sites of active angiogenesis in tumor. AC133+ cells labeled with ferumoxide-protamine sulfate were mixed with either rat glioma or human melanoma cells and implanted in flank of nude mice. An MRI of the tumors including surrounding tissues was performed. Tumor sections were stained for Prussian blue (PB), platelet-derived growth factor (PDGF), hypoxia-inducible factor-1α (HIF-1α), stromal cell derived factor-1 (SDF-1), matrix metalloproteinase-2 (MMP-2), vascular endothelial growth factor (VEGF), and endothelial markers. Fresh snap-frozen strips from the central and peripheral parts of the tumor were collected for Western blotting. MRIs demonstrated hypointense regions at the periphery of the tumors where the PB+/AC133+ cells were positive for endothelial cells markers. At the sites of PB+/AC133+ cells, both HIF-1α and SDF-1 were strongly positive and PDGF and MMP-2 showed generalized expression in the tumor and surrounding tissues. There was no significant association of PB+/AC133+ cell localization and VEGF expression in tumor cells. Western blot demonstrated strong expression of the SDF-1, MMP-2, and PDGF at the peripheral parts of the tumors. HIF-1α was expressed at both the periphery and central parts of the tumor. This work demonstrates that magnetically labeled cells can be used as probes for MRI and histological identification of administered cells. - Arbab, A. S., Janic, B., Knight, R. A., Anderson, S. A., Pawelczyk, E., Rad, A. M., Read, E. J., Pandit, S. D., Frank, J. A. Detection of migration of locally implanted AC133+ stem cells by cellular magnetic resonance imaging with histological findings.",
keywords = "Angiogenesis, MRI, Magnetically labeled AC133 cells, Tumors",
author = "Arbab, {Ali Syed} and Branislava Janic and Knight, {Robert A.} and Anderson, {Stasia A.} and Edyta Pawelczyk and Rad, {Ali M.} and Read, {Elizabeth J.} and Pandit, {Sunil D.} and Frank, {Joseph A.}",
year = "2008",
month = "9",
day = "1",
doi = "10.1096/fj.07-105676",
language = "English (US)",
volume = "22",
pages = "3234--3246",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "9",

}

TY - JOUR

T1 - Detection of migration of locally implanted AC133+ stem cells by cellular magnetic resonance imaging with histological findings

AU - Arbab, Ali Syed

AU - Janic, Branislava

AU - Knight, Robert A.

AU - Anderson, Stasia A.

AU - Pawelczyk, Edyta

AU - Rad, Ali M.

AU - Read, Elizabeth J.

AU - Pandit, Sunil D.

AU - Frank, Joseph A.

PY - 2008/9/1

Y1 - 2008/9/1

N2 - This study investigated the factors responsible for migration and homing of magnetically labeled AC133+ cells at the sites of active angiogenesis in tumor. AC133+ cells labeled with ferumoxide-protamine sulfate were mixed with either rat glioma or human melanoma cells and implanted in flank of nude mice. An MRI of the tumors including surrounding tissues was performed. Tumor sections were stained for Prussian blue (PB), platelet-derived growth factor (PDGF), hypoxia-inducible factor-1α (HIF-1α), stromal cell derived factor-1 (SDF-1), matrix metalloproteinase-2 (MMP-2), vascular endothelial growth factor (VEGF), and endothelial markers. Fresh snap-frozen strips from the central and peripheral parts of the tumor were collected for Western blotting. MRIs demonstrated hypointense regions at the periphery of the tumors where the PB+/AC133+ cells were positive for endothelial cells markers. At the sites of PB+/AC133+ cells, both HIF-1α and SDF-1 were strongly positive and PDGF and MMP-2 showed generalized expression in the tumor and surrounding tissues. There was no significant association of PB+/AC133+ cell localization and VEGF expression in tumor cells. Western blot demonstrated strong expression of the SDF-1, MMP-2, and PDGF at the peripheral parts of the tumors. HIF-1α was expressed at both the periphery and central parts of the tumor. This work demonstrates that magnetically labeled cells can be used as probes for MRI and histological identification of administered cells. - Arbab, A. S., Janic, B., Knight, R. A., Anderson, S. A., Pawelczyk, E., Rad, A. M., Read, E. J., Pandit, S. D., Frank, J. A. Detection of migration of locally implanted AC133+ stem cells by cellular magnetic resonance imaging with histological findings.

AB - This study investigated the factors responsible for migration and homing of magnetically labeled AC133+ cells at the sites of active angiogenesis in tumor. AC133+ cells labeled with ferumoxide-protamine sulfate were mixed with either rat glioma or human melanoma cells and implanted in flank of nude mice. An MRI of the tumors including surrounding tissues was performed. Tumor sections were stained for Prussian blue (PB), platelet-derived growth factor (PDGF), hypoxia-inducible factor-1α (HIF-1α), stromal cell derived factor-1 (SDF-1), matrix metalloproteinase-2 (MMP-2), vascular endothelial growth factor (VEGF), and endothelial markers. Fresh snap-frozen strips from the central and peripheral parts of the tumor were collected for Western blotting. MRIs demonstrated hypointense regions at the periphery of the tumors where the PB+/AC133+ cells were positive for endothelial cells markers. At the sites of PB+/AC133+ cells, both HIF-1α and SDF-1 were strongly positive and PDGF and MMP-2 showed generalized expression in the tumor and surrounding tissues. There was no significant association of PB+/AC133+ cell localization and VEGF expression in tumor cells. Western blot demonstrated strong expression of the SDF-1, MMP-2, and PDGF at the peripheral parts of the tumors. HIF-1α was expressed at both the periphery and central parts of the tumor. This work demonstrates that magnetically labeled cells can be used as probes for MRI and histological identification of administered cells. - Arbab, A. S., Janic, B., Knight, R. A., Anderson, S. A., Pawelczyk, E., Rad, A. M., Read, E. J., Pandit, S. D., Frank, J. A. Detection of migration of locally implanted AC133+ stem cells by cellular magnetic resonance imaging with histological findings.

KW - Angiogenesis

KW - MRI

KW - Magnetically labeled AC133 cells

KW - Tumors

UR - http://www.scopus.com/inward/record.url?scp=51349095580&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=51349095580&partnerID=8YFLogxK

U2 - 10.1096/fj.07-105676

DO - 10.1096/fj.07-105676

M3 - Article

VL - 22

SP - 3234

EP - 3246

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 9

ER -