Detection of prostatic‐inhibin‐like peptide in the cytoplasm of LNCaP cells, a human prostatic adenocarcinoma cell line

Kalpana S. Hurkadli, Anil R. Sheth, Balakrishna L Lokeshwar, Norman L. Block

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Prostatic inhibin peptide (PIP) is a 94‐amino acid peptide involved in various cellular functions. The concentration of this peptide changes with prostatic pathophysiology suggesting a role in various disease conditions; present study was undertaken to investigate the presence of this peptide in two human prostatic cell lines: LNCaP and PC3 cells. The LNCaP cells showed an intense intracellular fluorescence pattern after staining with rabbit‐anti‐PIP antiserum and FITC conjugated goat antirabbit‐IgG, while the PC3 cells did not exhibit any fluorescence. There was no alteration in the concentration of PIP in LNCaP cells with or without supplementation of steroids in culture medium. Immunoblot analysis indicates similarities between PIP from LNCaP cells and that from the human seminal plasma. Thus, present study demonstrates the presence of PIP in a human prostatic cell line, i.e., LNCaP cells. Its intracellular concentration is androgen independent, and has a close similarity with PIP isolated from the human seminal plasma. © 1994 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)285-290
Number of pages6
JournalThe Prostate
Volume24
Issue number6
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

Fingerprint

Cytoplasm
Adenocarcinoma
Cell Line
Peptides
Semen
Fluorescence
Fluorescein-5-isothiocyanate
Goats
Androgens
Culture Media
Immune Sera
Steroids
beta-microseminoprotein
Staining and Labeling
Acids

Keywords

  • LNCaP
  • PC3
  • inhibin

ASJC Scopus subject areas

  • Oncology
  • Urology

Cite this

Detection of prostatic‐inhibin‐like peptide in the cytoplasm of LNCaP cells, a human prostatic adenocarcinoma cell line. / Hurkadli, Kalpana S.; Sheth, Anil R.; Lokeshwar, Balakrishna L; Block, Norman L.

In: The Prostate, Vol. 24, No. 6, 01.01.1994, p. 285-290.

Research output: Contribution to journalArticle

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N2 - Prostatic inhibin peptide (PIP) is a 94‐amino acid peptide involved in various cellular functions. The concentration of this peptide changes with prostatic pathophysiology suggesting a role in various disease conditions; present study was undertaken to investigate the presence of this peptide in two human prostatic cell lines: LNCaP and PC3 cells. The LNCaP cells showed an intense intracellular fluorescence pattern after staining with rabbit‐anti‐PIP antiserum and FITC conjugated goat antirabbit‐IgG, while the PC3 cells did not exhibit any fluorescence. There was no alteration in the concentration of PIP in LNCaP cells with or without supplementation of steroids in culture medium. Immunoblot analysis indicates similarities between PIP from LNCaP cells and that from the human seminal plasma. Thus, present study demonstrates the presence of PIP in a human prostatic cell line, i.e., LNCaP cells. Its intracellular concentration is androgen independent, and has a close similarity with PIP isolated from the human seminal plasma. © 1994 Wiley‐Liss, Inc.

AB - Prostatic inhibin peptide (PIP) is a 94‐amino acid peptide involved in various cellular functions. The concentration of this peptide changes with prostatic pathophysiology suggesting a role in various disease conditions; present study was undertaken to investigate the presence of this peptide in two human prostatic cell lines: LNCaP and PC3 cells. The LNCaP cells showed an intense intracellular fluorescence pattern after staining with rabbit‐anti‐PIP antiserum and FITC conjugated goat antirabbit‐IgG, while the PC3 cells did not exhibit any fluorescence. There was no alteration in the concentration of PIP in LNCaP cells with or without supplementation of steroids in culture medium. Immunoblot analysis indicates similarities between PIP from LNCaP cells and that from the human seminal plasma. Thus, present study demonstrates the presence of PIP in a human prostatic cell line, i.e., LNCaP cells. Its intracellular concentration is androgen independent, and has a close similarity with PIP isolated from the human seminal plasma. © 1994 Wiley‐Liss, Inc.

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