Detection of specific bacterial antigen in urine of patients infected with Bacteroides fragilis

J. P. Rissing, T. B. Buxton, R. W. Harris, R. K. Shockley

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Abstract

An indirect enzyme-linked immunosorbent assay was developed to detect specific Bacteroides fragilis antigen(s) in human urine. Specimens collected within 72 hr of a positive culture were centrifuged, dialyzed, and treated with Tween 20, polyethylene glycol, and bovine serum albumin. Goat hyperimmune γ-globulin to B. fragilis strain ATCC 23745 was added and incubated, and supernatants were tested for antibody activity to polysaccharide-protein antigen of the same organism. Mean ± SD results, reported as percentage inhibition of control values and interpreted blindly, were as follows: 29 normal subjects, 9.8% ± 6.0%; 22 patients with Enterobacteriaceae bacteremia, 6.0% ± 5.1%; six patients with nonbacteremic infections due to B. fragilis, 22.3% ± 10.3%; and nine patients with B. fragilis bacteremia, 28.7% ± 10.2%. Three of six nonbacteremic patients and eight of nine bacteremic patients yielded values > 2 SD of control values. None of 22 patients with Enterobacteriaceae bacteremia was falsely positive (specificity, 100%).

Original languageEnglish (US)
Pages (from-to)929-934
Number of pages6
JournalJournal of Infectious Diseases
Volume149
Issue number6
DOIs
StatePublished - Jan 1 1984
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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