TY - JOUR
T1 - Detection of specific bacterial antigen in urine of patients infected with Bacteroides fragilis
AU - Rissing, J. P.
AU - Buxton, T. B.
AU - Harris, R. W.
AU - Shockley, R. K.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1984
Y1 - 1984
N2 - An indirect enzyme-linked immunosorbent assay was developed to detect specific Bacteroides fragilis antigen(s) in human urine. Specimens collected within 72 hr of a positive culture were centrifuged, dialyzed, and treated with Tween 20, polyethylene glycol, and bovine serum albumin. Goat hyperimmune γ-globulin to B. fragilis strain ATCC 23745 was added and incubated, and supernatants were tested for antibody activity to polysaccharide-protein antigen of the same organism. Mean ± SD results, reported as percentage inhibition of control values and interpreted blindly, were as follows: 29 normal subjects, 9.8% ± 6.0%; 22 patients with Enterobacteriaceae bacteremia, 6.0% ± 5.1%; six patients with nonbacteremic infections due to B. fragilis, 22.3% ± 10.3%; and nine patients with B. fragilis bacteremia, 28.7% ± 10.2%. Three of six nonbacteremic patients and eight of nine bacteremic patients yielded values > 2 SD of control values. None of 22 patients with Enterobacteriaceae bacteremia was falsely positive (specificity, 100%).
AB - An indirect enzyme-linked immunosorbent assay was developed to detect specific Bacteroides fragilis antigen(s) in human urine. Specimens collected within 72 hr of a positive culture were centrifuged, dialyzed, and treated with Tween 20, polyethylene glycol, and bovine serum albumin. Goat hyperimmune γ-globulin to B. fragilis strain ATCC 23745 was added and incubated, and supernatants were tested for antibody activity to polysaccharide-protein antigen of the same organism. Mean ± SD results, reported as percentage inhibition of control values and interpreted blindly, were as follows: 29 normal subjects, 9.8% ± 6.0%; 22 patients with Enterobacteriaceae bacteremia, 6.0% ± 5.1%; six patients with nonbacteremic infections due to B. fragilis, 22.3% ± 10.3%; and nine patients with B. fragilis bacteremia, 28.7% ± 10.2%. Three of six nonbacteremic patients and eight of nine bacteremic patients yielded values > 2 SD of control values. None of 22 patients with Enterobacteriaceae bacteremia was falsely positive (specificity, 100%).
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U2 - 10.1093/infdis/149.6.929
DO - 10.1093/infdis/149.6.929
M3 - Article
C2 - 6736683
AN - SCOPUS:0021268870
SN - 0022-1899
VL - 149
SP - 929
EP - 934
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 6
ER -