Detection of submicroscopic chromosomal deletions in aniridia patients using fluorescence in situ hybridization and a panel of cosmids covering the WT1 gene

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A series of cosmids have been isolated from a human chromosome 11-specific cosmid library using the human Wilms tumour predisposition gene cDNA, WT33. Seven overlapping cosmids were isolated which cover the genomic sequence of WT1 and in situ hybridisation shows that they all localise to the p13 region of chromosome 11. Chromosomes from patients with aniridia and Wilms tumour, and a small subband deletion in 11p13, were analysed and no hybridisation signal was seen on the deletion chromosomes. These cosmids, therefore, can be used to analyse chromosomes from patients with sporadic aniridia for submicroscopic deletions. Aniridia patients who show normal hybridisation patterns on both chromosomes need no longer be screened for Wilms tumours.

Original languageEnglish (US)
Pages (from-to)937-940
Number of pages4
JournalInternational Journal of Oncology
Volume3
Issue number5
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Aniridia
Cosmids
Fluorescence In Situ Hybridization
Chromosomes, Human, Pair 11
Wilms Tumor
Chromosomes
Genes
Wilms' Tumor Genes
Chromosome Deletion
Human Chromosomes
In Situ Hybridization
Complementary DNA

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

@article{3027d4e0442f4af4a8bea78ea7129de7,
title = "Detection of submicroscopic chromosomal deletions in aniridia patients using fluorescence in situ hybridization and a panel of cosmids covering the WT1 gene",
abstract = "A series of cosmids have been isolated from a human chromosome 11-specific cosmid library using the human Wilms tumour predisposition gene cDNA, WT33. Seven overlapping cosmids were isolated which cover the genomic sequence of WT1 and in situ hybridisation shows that they all localise to the p13 region of chromosome 11. Chromosomes from patients with aniridia and Wilms tumour, and a small subband deletion in 11p13, were analysed and no hybridisation signal was seen on the deletion chromosomes. These cosmids, therefore, can be used to analyse chromosomes from patients with sporadic aniridia for submicroscopic deletions. Aniridia patients who show normal hybridisation patterns on both chromosomes need no longer be screened for Wilms tumours.",
author = "H. Kempski and Cowell, {John Kenneth}",
year = "1993",
month = "1",
day = "1",
language = "English (US)",
volume = "3",
pages = "937--940",
journal = "International Journal of Oncology",
issn = "1019-6439",
publisher = "Spandidos Publications",
number = "5",

}

TY - JOUR

T1 - Detection of submicroscopic chromosomal deletions in aniridia patients using fluorescence in situ hybridization and a panel of cosmids covering the WT1 gene

AU - Kempski, H.

AU - Cowell, John Kenneth

PY - 1993/1/1

Y1 - 1993/1/1

N2 - A series of cosmids have been isolated from a human chromosome 11-specific cosmid library using the human Wilms tumour predisposition gene cDNA, WT33. Seven overlapping cosmids were isolated which cover the genomic sequence of WT1 and in situ hybridisation shows that they all localise to the p13 region of chromosome 11. Chromosomes from patients with aniridia and Wilms tumour, and a small subband deletion in 11p13, were analysed and no hybridisation signal was seen on the deletion chromosomes. These cosmids, therefore, can be used to analyse chromosomes from patients with sporadic aniridia for submicroscopic deletions. Aniridia patients who show normal hybridisation patterns on both chromosomes need no longer be screened for Wilms tumours.

AB - A series of cosmids have been isolated from a human chromosome 11-specific cosmid library using the human Wilms tumour predisposition gene cDNA, WT33. Seven overlapping cosmids were isolated which cover the genomic sequence of WT1 and in situ hybridisation shows that they all localise to the p13 region of chromosome 11. Chromosomes from patients with aniridia and Wilms tumour, and a small subband deletion in 11p13, were analysed and no hybridisation signal was seen on the deletion chromosomes. These cosmids, therefore, can be used to analyse chromosomes from patients with sporadic aniridia for submicroscopic deletions. Aniridia patients who show normal hybridisation patterns on both chromosomes need no longer be screened for Wilms tumours.

UR - http://www.scopus.com/inward/record.url?scp=0027360810&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027360810&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0027360810

VL - 3

SP - 937

EP - 940

JO - International Journal of Oncology

JF - International Journal of Oncology

SN - 1019-6439

IS - 5

ER -