Determination of chlorpyrifos and its metabolites in rat blood using liquid chromatography/electrospray ionization tandem mass spectrometry

Leah N. Williamson, Michael G. Bartlett, Alvin V Terry

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

A method has been developed to quantify chlorpyrifos (O,O-diethyl O-[3,5,6,-trichloro-2-pyridyl] phosphorothionate) and its metabolites chlorpyrifos-oxon (O,O-diethyl-O[3,5,6,trichloro-2-pyridyl] phosphate) and TCP (3,5,6,-trichloro-2-pyridinol) in rat blood by liquid chromatography/ electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS). Rat blood was treated by liquid-liquid extraction and the analytes were separated by gradient elution on an Agilent Zorbax Extended-C8 column (2.0×150 mm, 5 μm). Chlorpyrifos and chlorpyrifos-oxon were detected in positive ion mode using multiple reaction monitoring (MRM). TCP was detected in negative ion mode using parent-to-parent transition monitoring.This method was validated and the specificity, linearity, limit of quantitation (LOQ), precision, accuracy, stability, and recoveries were determined. The method was then applied to determine the level of chlorpyrifos and its metabolites from rats exposed to a subcutaneous injection of 10 and 18 mg/kg.

Original languageEnglish (US)
Pages (from-to)273-285
Number of pages13
JournalJournal of Liquid Chromatography and Related Technologies
Volume30
Issue number2
DOIs
StatePublished - Jan 1 2007

Keywords

  • Chlorpyrifos
  • Electrospray ionization
  • HPLC
  • Liquid-liquid extraction
  • Organophosphate insecticides
  • Tandem mass spectrometry

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Determination of chlorpyrifos and its metabolites in rat blood using liquid chromatography/electrospray ionization tandem mass spectrometry'. Together they form a unique fingerprint.

  • Cite this