Determination of labetalol hydrochloride by kinetic spectrophotometry using potassium permanganate as oxidant

Nafisur Rahman; Nishat Anwar; Mohammad Kashif; Md Nasrul Hoda; Habibur Rahman

Determination of labetalol hydrochloride by kinetic spectrophotometry using potassium permanganate as oxidant

Nafisur Rahman, Nishat Anwar, Mohammad Kashif, Md Nasrul Hoda, Habibur Rahman

Medical Laboratory, Imaging and Radiologic Sciences

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

A simple and sensitive kinetic spectrophotometric method was developed for the determination of labetalol (LBT) hydrochloride. The method was based on the kinetic investigation of the oxidation of the drug with alkaline potassium permanganate at room temperature (25 ± 1°C). The increase in absorbance of coloured manganate ions was measured at 605 nm. All experimental variables affecting the development of the colour were investigated and optimized. The initial rate and fixed time (at 6 minute) methods were adopted for determining the drug concentration. The calibration graphs were linear in the concentration ranges of 2-14 μg mL^-1 and 1-10 μg mL^-1, using the initial rate and fixed time methods, respectively. The method was successfully applied to the determination of labetalol in laboratory made tablets and commercial tablets. The results were validated statistically and through recovery studies.

Original language	English (US)
Pages (from-to)	105-112
Number of pages	8
Journal	Journal of the Mexican Chemical Society
Volume	55
Issue number	2
State	Published - Apr 1 2011

Keywords

Initial rate and fixed time method
Kinetic spectrophotometry
Labetalol hydrochloride

ASJC Scopus subject areas

Chemistry(all)

Cite this

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title = "Determination of labetalol hydrochloride by kinetic spectrophotometry using potassium permanganate as oxidant",

abstract = "A simple and sensitive kinetic spectrophotometric method was developed for the determination of labetalol (LBT) hydrochloride. The method was based on the kinetic investigation of the oxidation of the drug with alkaline potassium permanganate at room temperature (25 ± 1°C). The increase in absorbance of coloured manganate ions was measured at 605 nm. All experimental variables affecting the development of the colour were investigated and optimized. The initial rate and fixed time (at 6 minute) methods were adopted for determining the drug concentration. The calibration graphs were linear in the concentration ranges of 2-14 μg mL-1 and 1-10 μg mL-1, using the initial rate and fixed time methods, respectively. The method was successfully applied to the determination of labetalol in laboratory made tablets and commercial tablets. The results were validated statistically and through recovery studies.",

keywords = "Initial rate and fixed time method, Kinetic spectrophotometry, Labetalol hydrochloride",

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T1 - Determination of labetalol hydrochloride by kinetic spectrophotometry using potassium permanganate as oxidant

AU - Rahman, Nafisur

AU - Anwar, Nishat

AU - Kashif, Mohammad

AU - Hoda, Md Nasrul

AU - Rahman, Habibur

PY - 2011/4/1

Y1 - 2011/4/1

N2 - A simple and sensitive kinetic spectrophotometric method was developed for the determination of labetalol (LBT) hydrochloride. The method was based on the kinetic investigation of the oxidation of the drug with alkaline potassium permanganate at room temperature (25 ± 1°C). The increase in absorbance of coloured manganate ions was measured at 605 nm. All experimental variables affecting the development of the colour were investigated and optimized. The initial rate and fixed time (at 6 minute) methods were adopted for determining the drug concentration. The calibration graphs were linear in the concentration ranges of 2-14 μg mL-1 and 1-10 μg mL-1, using the initial rate and fixed time methods, respectively. The method was successfully applied to the determination of labetalol in laboratory made tablets and commercial tablets. The results were validated statistically and through recovery studies.

AB - A simple and sensitive kinetic spectrophotometric method was developed for the determination of labetalol (LBT) hydrochloride. The method was based on the kinetic investigation of the oxidation of the drug with alkaline potassium permanganate at room temperature (25 ± 1°C). The increase in absorbance of coloured manganate ions was measured at 605 nm. All experimental variables affecting the development of the colour were investigated and optimized. The initial rate and fixed time (at 6 minute) methods were adopted for determining the drug concentration. The calibration graphs were linear in the concentration ranges of 2-14 μg mL-1 and 1-10 μg mL-1, using the initial rate and fixed time methods, respectively. The method was successfully applied to the determination of labetalol in laboratory made tablets and commercial tablets. The results were validated statistically and through recovery studies.

KW - Initial rate and fixed time method

KW - Kinetic spectrophotometry

KW - Labetalol hydrochloride

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ER -

Determination of labetalol hydrochloride by kinetic spectrophotometry using potassium permanganate as oxidant

Abstract

Keywords

ASJC Scopus subject areas

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