Abstract
Objective: To introduce a simple, rapid and stable method for the determination of plasma acylation stimulating protein (ASP) and to explore the value of plasma ASP determination. Methods: Sixty-three simple obese children and 98 healthy controls were included in this study. The ELISA technique was employed to determine the plasma ASP concentration. Results: The best linearity of test was between 0.5-10 ng/mL. The intra-assay coefficient of variation (CV) was 8.17% and inter-assay CV, 9.72%. The average plasma ASP concentration in healthy children was 69.14 ± 25.58 nM and 95.64 ± 36.24 nM in obese children (P <0.001). Plasma ASP was positively correlated with body mass index in both healthy and obese children (r = 0.2883 and 0.337, respectively, both P < 0.01). Conclusions: Plasma ASP can be quantified by the ELISA method. Plasma ASP determination may be recommended as a new method for lipid. metabolism determination in children.
Original language | English (US) |
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Pages (from-to) | 408-410 |
Number of pages | 3 |
Journal | Chinese Journal of Contemporary Pediatrics |
Volume | 7 |
Issue number | 5 |
State | Published - Oct 2005 |
Externally published | Yes |
Keywords
- Acylation stimulating protein
- Child
- Plasma
- Simple obesity
ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health