Determination of reduced scattering coefficient of biological tissue from a needle-like probe

Maureen Johns, Cole A. Giller, Dwighl C. German, Hanli Liu

Research output: Contribution to journalArticle

90 Scopus citations


Detection of interactions between light and tissue can be used to characterize the optical properties of the tissue. The purpose of this paper is to develop an algorithm that determines the reduced scattering coefficient (μs') of tissues from a single optical reflectance spectrum measured with a small source-detector separation. A qualitative relationship between μs' and optical reflectance was developed using both Monte Carlo simulations and empirical tissue calibrations for each of two fiber optic probes with 400-μm and 100-μm fibers. Optical reflectance measurements, using a standard frequency-domain oximeter, were performed to validate the calculated μs' values. The algorithm was useful for determining μs' values of in vivo human fingers and rat brain tissues.

Original languageEnglish (US)
Pages (from-to)4828-4842
Number of pages15
JournalOptics Express
Issue number13
StatePublished - Jun 27 2005
Externally publishedYes


ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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