Development and characterization of monoclonal antibodies to murine macrophage colony-stimulating factor

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The macrophage-specific CSF (CSF-1), purified from murine L cell-conditioned medium, supports the in vitro proliferation and survival of various murine mononuclear phagocyte colony-forming cells. In this report we describe the productional and functional characterization of two monoclonal antibodies (mAb) to CSF-1 obtained from rat x rat hybridomas. These two mAb are fucntionally distinct and recognize different epitopes on CSF-1. The mAb 5A1 binds to and inhibits the biologic function of CSF-1, and the second mAb (D24) binds CSF-1 but does not neutralize its biologic activity. The mAb 5A1 inhibits colony formation of tissue mononuclear phagocyte colony-forming cells as well as the committed bone marrow stem cells for both granulocytes and monocytes. The extent of colony inhibition by mAb 5A1 is dependent on the tissue origin of colony-forming cells. CSF-1 complexed with mAb 5A1 does not bind to its cell surface receptor of peritoneal exudate macrophages, and mAb 5A1 does not complex with cell-bound CSF-1. Although both bone marrow cell-derived macrophages and J774.1 macrophages bind CSF-1, mAb 5A1 inhibits the proliferation of only bone marrow cell-derived macrophages. The non-neutralizing mAb D24 does not block binding of CSF-1 to its cellular receptor, and it recognizes cell-bound CSF-1.

Original languageEnglish (US)
Pages (from-to)483-488
Number of pages6
JournalJournal of Immunology
Volume141
Issue number2
StatePublished - Jan 1 1988
Externally publishedYes

Fingerprint

Macrophage Colony-Stimulating Factor
Monoclonal Antibodies
Macrophages
Bone Marrow Cells
Phagocytes
Hybridomas
Peritoneal Macrophages
Cell Surface Receptors
Exudates and Transudates
Conditioned Culture Medium
Granulocytes
Epitopes
Monocytes
Stem Cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Development and characterization of monoclonal antibodies to murine macrophage colony-stimulating factor. / Lokeshwar, Balakrishna L; Lin, H. S.

In: Journal of Immunology, Vol. 141, No. 2, 01.01.1988, p. 483-488.

Research output: Contribution to journalArticle

@article{2f848cc0b67b4090be361218d3e30932,
title = "Development and characterization of monoclonal antibodies to murine macrophage colony-stimulating factor",
abstract = "The macrophage-specific CSF (CSF-1), purified from murine L cell-conditioned medium, supports the in vitro proliferation and survival of various murine mononuclear phagocyte colony-forming cells. In this report we describe the productional and functional characterization of two monoclonal antibodies (mAb) to CSF-1 obtained from rat x rat hybridomas. These two mAb are fucntionally distinct and recognize different epitopes on CSF-1. The mAb 5A1 binds to and inhibits the biologic function of CSF-1, and the second mAb (D24) binds CSF-1 but does not neutralize its biologic activity. The mAb 5A1 inhibits colony formation of tissue mononuclear phagocyte colony-forming cells as well as the committed bone marrow stem cells for both granulocytes and monocytes. The extent of colony inhibition by mAb 5A1 is dependent on the tissue origin of colony-forming cells. CSF-1 complexed with mAb 5A1 does not bind to its cell surface receptor of peritoneal exudate macrophages, and mAb 5A1 does not complex with cell-bound CSF-1. Although both bone marrow cell-derived macrophages and J774.1 macrophages bind CSF-1, mAb 5A1 inhibits the proliferation of only bone marrow cell-derived macrophages. The non-neutralizing mAb D24 does not block binding of CSF-1 to its cellular receptor, and it recognizes cell-bound CSF-1.",
author = "Lokeshwar, {Balakrishna L} and Lin, {H. S.}",
year = "1988",
month = "1",
day = "1",
language = "English (US)",
volume = "141",
pages = "483--488",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "2",

}

TY - JOUR

T1 - Development and characterization of monoclonal antibodies to murine macrophage colony-stimulating factor

AU - Lokeshwar, Balakrishna L

AU - Lin, H. S.

PY - 1988/1/1

Y1 - 1988/1/1

N2 - The macrophage-specific CSF (CSF-1), purified from murine L cell-conditioned medium, supports the in vitro proliferation and survival of various murine mononuclear phagocyte colony-forming cells. In this report we describe the productional and functional characterization of two monoclonal antibodies (mAb) to CSF-1 obtained from rat x rat hybridomas. These two mAb are fucntionally distinct and recognize different epitopes on CSF-1. The mAb 5A1 binds to and inhibits the biologic function of CSF-1, and the second mAb (D24) binds CSF-1 but does not neutralize its biologic activity. The mAb 5A1 inhibits colony formation of tissue mononuclear phagocyte colony-forming cells as well as the committed bone marrow stem cells for both granulocytes and monocytes. The extent of colony inhibition by mAb 5A1 is dependent on the tissue origin of colony-forming cells. CSF-1 complexed with mAb 5A1 does not bind to its cell surface receptor of peritoneal exudate macrophages, and mAb 5A1 does not complex with cell-bound CSF-1. Although both bone marrow cell-derived macrophages and J774.1 macrophages bind CSF-1, mAb 5A1 inhibits the proliferation of only bone marrow cell-derived macrophages. The non-neutralizing mAb D24 does not block binding of CSF-1 to its cellular receptor, and it recognizes cell-bound CSF-1.

AB - The macrophage-specific CSF (CSF-1), purified from murine L cell-conditioned medium, supports the in vitro proliferation and survival of various murine mononuclear phagocyte colony-forming cells. In this report we describe the productional and functional characterization of two monoclonal antibodies (mAb) to CSF-1 obtained from rat x rat hybridomas. These two mAb are fucntionally distinct and recognize different epitopes on CSF-1. The mAb 5A1 binds to and inhibits the biologic function of CSF-1, and the second mAb (D24) binds CSF-1 but does not neutralize its biologic activity. The mAb 5A1 inhibits colony formation of tissue mononuclear phagocyte colony-forming cells as well as the committed bone marrow stem cells for both granulocytes and monocytes. The extent of colony inhibition by mAb 5A1 is dependent on the tissue origin of colony-forming cells. CSF-1 complexed with mAb 5A1 does not bind to its cell surface receptor of peritoneal exudate macrophages, and mAb 5A1 does not complex with cell-bound CSF-1. Although both bone marrow cell-derived macrophages and J774.1 macrophages bind CSF-1, mAb 5A1 inhibits the proliferation of only bone marrow cell-derived macrophages. The non-neutralizing mAb D24 does not block binding of CSF-1 to its cellular receptor, and it recognizes cell-bound CSF-1.

UR - http://www.scopus.com/inward/record.url?scp=0023811311&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023811311&partnerID=8YFLogxK

M3 - Article

C2 - 2454994

AN - SCOPUS:0023811311

VL - 141

SP - 483

EP - 488

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 2

ER -