Development and evaluation of an enzyme-linked immunosorbent assay with recombinant SAG2 for diagnosis of Toxoplasma gondii infection in cats

X. Huang, X. Xuan, E. N. Kimbita, B. Battur, T. Miyazawa, S. Fukumoto, M. Mishima, Levi H Makala, H. Suzuki, C. Sugimoto, H. Nagasawa, K. Fujisaki, T. Mikami, I. Igarashi

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Abstract

Cats are pivotal in the transmission of Toxoplasma gondii. To develop a sensitive and specific serodiagnostic method for feline toxoplasmosis, surface antigen 2 (SAG2) of T. gondii was expressed in Escherichia coli and its diagnostic potential evaluated in an enzyme-linked immunosorbent assay (ELISA). The ELISA with recombinant SAG2 (rSAG2) was able to differentiate very clearly between sera from cats experimentally infected with T. gondii and sera from normal cats. Serum samples collected from domestic cats in Japan were investigated by the ELISA, and the results were compared with those of a commercially available latex agglutination test (LAT) kit. Of the 192 samples screened, 42 (21.9%) were positive by ELISA. Among the 42 ELISA-positive samples, 39 were positive by LAT. There was a significant correlation between ELISA and LAT titers. All the 150 ELISA-negative samples were negative by LAT. These results indicate that the ELISA with rSAG2 expressed in E. coli should be a useful method for detection of T. gondii infection in cats.

Original languageEnglish (US)
Pages (from-to)804-807
Number of pages4
JournalJournal of Parasitology
Volume88
Issue number4
DOIs
StatePublished - Jan 1 2002

Fingerprint

recombinant antigens
Toxoplasmosis
surface antigens
Surface Antigens
Toxoplasma gondii
antigen
Cats
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
assay
cats
enzyme
Latex Fixation Tests
latex agglutination test
infection
Toxoplasma
serum
Serum
Escherichia coli
sampling

ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Development and evaluation of an enzyme-linked immunosorbent assay with recombinant SAG2 for diagnosis of Toxoplasma gondii infection in cats. / Huang, X.; Xuan, X.; Kimbita, E. N.; Battur, B.; Miyazawa, T.; Fukumoto, S.; Mishima, M.; Makala, Levi H; Suzuki, H.; Sugimoto, C.; Nagasawa, H.; Fujisaki, K.; Mikami, T.; Igarashi, I.

In: Journal of Parasitology, Vol. 88, No. 4, 01.01.2002, p. 804-807.

Research output: Contribution to journalArticle

Huang, X, Xuan, X, Kimbita, EN, Battur, B, Miyazawa, T, Fukumoto, S, Mishima, M, Makala, LH, Suzuki, H, Sugimoto, C, Nagasawa, H, Fujisaki, K, Mikami, T & Igarashi, I 2002, 'Development and evaluation of an enzyme-linked immunosorbent assay with recombinant SAG2 for diagnosis of Toxoplasma gondii infection in cats', Journal of Parasitology, vol. 88, no. 4, pp. 804-807. https://doi.org/10.1645/0022-3395(2002)088[0804:DAEOAE]2.0.CO;2
Huang, X. ; Xuan, X. ; Kimbita, E. N. ; Battur, B. ; Miyazawa, T. ; Fukumoto, S. ; Mishima, M. ; Makala, Levi H ; Suzuki, H. ; Sugimoto, C. ; Nagasawa, H. ; Fujisaki, K. ; Mikami, T. ; Igarashi, I. / Development and evaluation of an enzyme-linked immunosorbent assay with recombinant SAG2 for diagnosis of Toxoplasma gondii infection in cats. In: Journal of Parasitology. 2002 ; Vol. 88, No. 4. pp. 804-807.
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