Development of a rat model of bisphosphonate-related osteonecrosis of the Jaw (BRONJ)

Karen L. Marino, Ibrahim Zakhary, Rafik A Abdelsayed, Jared A. Carter, Jack C. O'Neill, Rania M. Khashaba, Mohammed Elsayed Elsalanty, Mark R Stevens, James L. Borke

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The purpose of this study was to develop a rat model predictive of bisphosphonate-related osteonecrosis of the jaw (BRONJ) after exodontias. Thirty female rats were randomized into 2 groups, control and experimental. The experimental group received 2 intravenous injections of zoledronate (20 lg/kg). The mesial root of the right mandibular first molar was extracted. Rats were euthanized at 0, 4, and 8 weeks. Bone mineral density (BMD), collagen breakdown (pyridinium [PYD]), vascular regeneration (VEGF), and histology were examined. A trend toward higher PYD values was suggested in control vs experimental groups after wounding. Serum VEGF increased significantly after wounding for both control and experimental groups. After 8 weeks, VEGF continued to rise for the experimental group only. In the extraction socket area, BMD was significantly lower after wounding in control vs. zoledronate-treated rats. Histology sections from experimental groups showed bacteria and bone necrosis. Consistent findings of BRONJ features similar to those in humans were observed after zoledronate treatment.

Original languageEnglish (US)
Pages (from-to)511-518
Number of pages8
JournalJournal of Oral Implantology
Volume38
Issue numberSUPPL. 1
DOIs
StatePublished - Jan 1 2012

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zoledronic acid
Bisphosphonate-Associated Osteonecrosis of the Jaw
Vascular Endothelial Growth Factor A
Bone Density
Histology
Control Groups
Osteonecrosis
Intravenous Injections
Blood Vessels
Regeneration
Collagen
Bacteria
Serum

Keywords

  • Bisphosphonate
  • BRONJ
  • Osteonecrosis of the jaw
  • Rat model

ASJC Scopus subject areas

  • Oral Surgery

Cite this

Marino, K. L., Zakhary, I., Abdelsayed, R. A., Carter, J. A., O'Neill, J. C., Khashaba, R. M., ... Borke, J. L. (2012). Development of a rat model of bisphosphonate-related osteonecrosis of the Jaw (BRONJ). Journal of Oral Implantology, 38(SUPPL. 1), 511-518. https://doi.org/10.1563/AAID-JOI-D-11-00057

Development of a rat model of bisphosphonate-related osteonecrosis of the Jaw (BRONJ). / Marino, Karen L.; Zakhary, Ibrahim; Abdelsayed, Rafik A; Carter, Jared A.; O'Neill, Jack C.; Khashaba, Rania M.; Elsalanty, Mohammed Elsayed; Stevens, Mark R; Borke, James L.

In: Journal of Oral Implantology, Vol. 38, No. SUPPL. 1, 01.01.2012, p. 511-518.

Research output: Contribution to journalArticle

Marino, Karen L. ; Zakhary, Ibrahim ; Abdelsayed, Rafik A ; Carter, Jared A. ; O'Neill, Jack C. ; Khashaba, Rania M. ; Elsalanty, Mohammed Elsayed ; Stevens, Mark R ; Borke, James L. / Development of a rat model of bisphosphonate-related osteonecrosis of the Jaw (BRONJ). In: Journal of Oral Implantology. 2012 ; Vol. 38, No. SUPPL. 1. pp. 511-518.
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