Development of an allogeneic adherent stem cell therapy for treatment of ischemic stroke

Robert W. Mays, Cesar V. Borlongan, Takao Yasuhara, Koichi Hara, Mina Maki, James E. Carroll, Robert J. Deans, David C. Hess

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

To determine the potential for using stem cells in the treatment of ischemic injuries of the central nervous system, clinically relevant experiments were performed administering human adult adherent bone marrow derived stem cells into a rat model of ischemic stroke. Variables such as the requirement for immunosuppression, route of cell administration, window for therapeutic benefit and optimal cell dosage have all been examined in a series of relevant translational experiments in animals undergoing middle cerebral artery ligation stroke injury. Animals were tested for improvements in locomotor and neurological function at time points as late as 6 months post-cell transplantation and demonstrated sustained statistically significant benefit from a single dose of cells. Following sacrifice, immunohistochemistry was performed on tissues to determine stem cell engraftment and fate, as well as neuroprotection of endogenous tissue at the sites of ischemic injury. The results indicate locomotor and neurological improvement correlates with improved neuroprotection and limited retention of the transplanted stem cells, with limited neuronal fate. The observed benefit occurs in a cell dosage dependent fashion suggesting a pharmacological role for the cells when administered intravenously in these injury models.

Original languageEnglish (US)
Pages (from-to)34-46
Number of pages13
JournalJournal of Experimental Stroke and Translational Medicine
Volume3
Issue number1
DOIs
StatePublished - 2010

Keywords

  • Ischemic stroke
  • Neuroprotection
  • Stem and progenitor cells
  • Translational research
  • Xenogeneic graft

ASJC Scopus subject areas

  • Neuroscience (miscellaneous)
  • Neurology
  • Clinical Neurology
  • Cognitive Neuroscience

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