Development of an in vivo model of laryngeal burn injury

Gregory R. Dion, Stephanie Teng, Renjie Bing, Nao Hiwatashi, Milan R. Amin, Ryan C. Branski

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objectives/Hypothesis: Inhalation injury significantly increases morbidity and mortality in burn patients. Approximately one in five burn patients have acute injury to the larynx, trachea, and/or lungs—and as many as 70% have long-term laryngeal abnormalities. Although inhalation injury to the lung has been studied extensively, no models exist to study these insults to the larynx. As such, we developed an in vivo rabbit model to create precise and reproducible laryngeal burn with resultant tissue damage as a foundation for interventional studies. Methods: Following tubeless tracheotomy, a custom temperature-control device was employed to apply heated air (70°C–80°C, 150°C–160°C, or 310°C–320°C) ± smoke derived from unbleached cotton to the larynx, endoscopically, minimizing adjacent tissue damage in six rabbits. Pain, nutrition, and level of activity were monitored. Direct laryngoscopy and histological examination were performed 24 hours following insult. Results: All animals survived injury with appropriate pain control; oral intake was initiated and all were adequately ventilating via tracheostomy. Burn sequelae were noted under direct visualization 24 hours after injury, and graded levels of edema and tissue damage were observed as a function of temperature. Edema obstructed true vocal fold visualization at increased temperatures. These injury patterns correlated with graded tissue damage on histology. Conclusion: We created an in vivo model of laryngeal burn injury employing a custom burn device resulting in graded tissue injury. This model is critical for investigation of the mechanisms underlying burn injury, and ultimately, the development and evaluation of therapies for this challenging population. Level of Evidence: NA Laryngoscope, 127:186–190, 2017.

Original languageEnglish (US)
Pages (from-to)186-190
Number of pages5
JournalLaryngoscope
Volume127
Issue number1
DOIs
StatePublished - Jan 1 2017

Fingerprint

Wounds and Injuries
Larynx
Inhalation
Temperature
Edema
Rabbits
Laryngoscopes
Pain
Equipment and Supplies
Tracheotomy
Laryngoscopy
Vocal Cords
Tracheostomy
Lung Injury
Trachea
Smoke
Histology
Air
Morbidity
Mortality

Keywords

  • Airway burn
  • burn
  • burn model
  • inhalation burn
  • laryngeal burn
  • larynx

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Dion, G. R., Teng, S., Bing, R., Hiwatashi, N., Amin, M. R., & Branski, R. C. (2017). Development of an in vivo model of laryngeal burn injury. Laryngoscope, 127(1), 186-190. https://doi.org/10.1002/lary.26123

Development of an in vivo model of laryngeal burn injury. / Dion, Gregory R.; Teng, Stephanie; Bing, Renjie; Hiwatashi, Nao; Amin, Milan R.; Branski, Ryan C.

In: Laryngoscope, Vol. 127, No. 1, 01.01.2017, p. 186-190.

Research output: Contribution to journalArticle

Dion, GR, Teng, S, Bing, R, Hiwatashi, N, Amin, MR & Branski, RC 2017, 'Development of an in vivo model of laryngeal burn injury', Laryngoscope, vol. 127, no. 1, pp. 186-190. https://doi.org/10.1002/lary.26123
Dion GR, Teng S, Bing R, Hiwatashi N, Amin MR, Branski RC. Development of an in vivo model of laryngeal burn injury. Laryngoscope. 2017 Jan 1;127(1):186-190. https://doi.org/10.1002/lary.26123
Dion, Gregory R. ; Teng, Stephanie ; Bing, Renjie ; Hiwatashi, Nao ; Amin, Milan R. ; Branski, Ryan C. / Development of an in vivo model of laryngeal burn injury. In: Laryngoscope. 2017 ; Vol. 127, No. 1. pp. 186-190.
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