Development of an injectable composite as a carrier for growth factor-enhanced periodontal regeneration

Samuel Herberg, Michael Siedler, Susanne Pippig, Andreas Schuetz, Carola Dony, Chong Kwan Kim, Ulf M E Wikesjö

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Aim: Biomaterials are often applied in periodontal therapy; however, not always well adapted for tissue regeneration. The objective of this study was to evaluate the physico-chemical properties and biocompatibility of an injectable, in situ setting composite for growth factor-enhanced periodontal regeneration. Material and Methods: The composite constitutes bioresorbable poly(lactic-co-glycolic acid) (PLGA) and additives forming in situ a matrix designed as a carrier for recombinant human growth/differentiation factor-5 (rhGDF-5). In vitro characterization included the porosity, biointeraction, biodegradation, injectability, and biological activity of released rhGDF-5. Biocompatibility was compared with granular β-tricalcium phosphate and an absorbable collagen sponge using a canine periodontal defect model. Results: The PLGA composite showed a highly porous (500-1000 μm) space-providing structure. It effectively induced coagulation exhibiting an intimate interaction with the fibrin clot. The biphasic biodegradation was complete within 4 weeks. The composite was conveniently injectable (90.4±3.6 N) for ease of use. It exhibited a sustained rhGDF-5 release over 4 weeks (40.8%) after initial burst (3.4%) detected by ALP activity. Sites receiving the composite showed limited, if any, residuals and had no appreciable negative effect on periodontal wound healing. There were no noteworthy inflammatory lesions in sites receiving the PLGA composite. Conclusion: Characteristics of the PLGA composite makes it an attractive matrix to support native wound healing and rhGDF-5-enhanced periodontal regeneration.

Original languageEnglish (US)
Pages (from-to)976-984
Number of pages9
JournalJournal of Clinical Periodontology
Volume35
Issue number11
DOIs
StatePublished - Jan 1 2008

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Regeneration
Intercellular Signaling Peptides and Proteins
Injections
Wound Healing
Porosity
Porifera
Biocompatible Materials
Fibrin
Canidae
Collagen
human GDF5 protein
polylactic acid-polyglycolic acid copolymer
Therapeutics

Keywords

  • Bioresorbable
  • Blood clot
  • In situ setting
  • Minimally invasive
  • PLGA
  • Periodontal regeneration
  • Space providing
  • rhGDF-5

ASJC Scopus subject areas

  • Periodontics

Cite this

Development of an injectable composite as a carrier for growth factor-enhanced periodontal regeneration. / Herberg, Samuel; Siedler, Michael; Pippig, Susanne; Schuetz, Andreas; Dony, Carola; Kim, Chong Kwan; Wikesjö, Ulf M E.

In: Journal of Clinical Periodontology, Vol. 35, No. 11, 01.01.2008, p. 976-984.

Research output: Contribution to journalArticle

Herberg, Samuel ; Siedler, Michael ; Pippig, Susanne ; Schuetz, Andreas ; Dony, Carola ; Kim, Chong Kwan ; Wikesjö, Ulf M E. / Development of an injectable composite as a carrier for growth factor-enhanced periodontal regeneration. In: Journal of Clinical Periodontology. 2008 ; Vol. 35, No. 11. pp. 976-984.
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