Developmental Changes in Ganglioside Composition and Synthesis in Embryonic Rat Brain

Robert K Yu, Lawrence J. Macala, Takao Taki, Henry M. Weinfeld, Franklin S. Yu

Research output: Contribution to journalArticle

229 Citations (Scopus)

Abstract

Abstract: Developmental changes in ganglioside composition and biosynthesis were studied in rat brain between embryonic day (E) 14 and birth. In E14 brains, GM3 and GD3 were predominant. At E16, “b” series gangliosides, such as GD1b, GT1b, and GQ1b, increased in content. After E18, “a” series gangliosides such as GM1, GD1a, and GT1a increased in content, and the content of GM3 and GD3 markedly decreased. Because of these changes in composition, we determined the activities, in homogenates of embryonic brains, of two key enzymes of ganglioside synthesis: sialyltransferase for the synthesis of GD3 from GM3 and N‐acetylgalactosaminyltransferase for GM2 synthesis from GM3. The sialyltransferase activity (GM3 → GD3) was constant between E14 and E18 but decreased rapidly from E18 to birth. In contrast, the N‐acetylgalacto‐saminyltransferase activity (GM3 ± GM2) increased between E14 and E18 but was constant from E18 to birth. These changes in ganglioside composition and enzymatic activities indicate that during development there is a shift from synthesis of the simplest gangliosides of the “a” and “b” pathways to synthesis of the more complex gangliosides.

Original languageEnglish (US)
Pages (from-to)1825-1829
Number of pages5
JournalJournal of Neurochemistry
Volume50
Issue number6
DOIs
StatePublished - Jan 1 1988

Fingerprint

Gangliosides
Rats
Brain
Chemical analysis
Sialyltransferases
Parturition
Biosynthesis
Enzymes

Keywords

  • Brain development
  • Gangliosides
  • N‐Acetylgalactosaminyltransferase
  • Sialyltransferase

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Developmental Changes in Ganglioside Composition and Synthesis in Embryonic Rat Brain. / Yu, Robert K; Macala, Lawrence J.; Taki, Takao; Weinfeld, Henry M.; Yu, Franklin S.

In: Journal of Neurochemistry, Vol. 50, No. 6, 01.01.1988, p. 1825-1829.

Research output: Contribution to journalArticle

Yu, Robert K ; Macala, Lawrence J. ; Taki, Takao ; Weinfeld, Henry M. ; Yu, Franklin S. / Developmental Changes in Ganglioside Composition and Synthesis in Embryonic Rat Brain. In: Journal of Neurochemistry. 1988 ; Vol. 50, No. 6. pp. 1825-1829.
@article{702827efad4b491ba2001221296d766c,
title = "Developmental Changes in Ganglioside Composition and Synthesis in Embryonic Rat Brain",
abstract = "Abstract: Developmental changes in ganglioside composition and biosynthesis were studied in rat brain between embryonic day (E) 14 and birth. In E14 brains, GM3 and GD3 were predominant. At E16, “b” series gangliosides, such as GD1b, GT1b, and GQ1b, increased in content. After E18, “a” series gangliosides such as GM1, GD1a, and GT1a increased in content, and the content of GM3 and GD3 markedly decreased. Because of these changes in composition, we determined the activities, in homogenates of embryonic brains, of two key enzymes of ganglioside synthesis: sialyltransferase for the synthesis of GD3 from GM3 and N‐acetylgalactosaminyltransferase for GM2 synthesis from GM3. The sialyltransferase activity (GM3 → GD3) was constant between E14 and E18 but decreased rapidly from E18 to birth. In contrast, the N‐acetylgalacto‐saminyltransferase activity (GM3 ± GM2) increased between E14 and E18 but was constant from E18 to birth. These changes in ganglioside composition and enzymatic activities indicate that during development there is a shift from synthesis of the simplest gangliosides of the “a” and “b” pathways to synthesis of the more complex gangliosides.",
keywords = "Brain development, Gangliosides, N‐Acetylgalactosaminyltransferase, Sialyltransferase",
author = "Yu, {Robert K} and Macala, {Lawrence J.} and Takao Taki and Weinfeld, {Henry M.} and Yu, {Franklin S.}",
year = "1988",
month = "1",
day = "1",
doi = "10.1111/j.1471-4159.1988.tb02484.x",
language = "English (US)",
volume = "50",
pages = "1825--1829",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Developmental Changes in Ganglioside Composition and Synthesis in Embryonic Rat Brain

AU - Yu, Robert K

AU - Macala, Lawrence J.

AU - Taki, Takao

AU - Weinfeld, Henry M.

AU - Yu, Franklin S.

PY - 1988/1/1

Y1 - 1988/1/1

N2 - Abstract: Developmental changes in ganglioside composition and biosynthesis were studied in rat brain between embryonic day (E) 14 and birth. In E14 brains, GM3 and GD3 were predominant. At E16, “b” series gangliosides, such as GD1b, GT1b, and GQ1b, increased in content. After E18, “a” series gangliosides such as GM1, GD1a, and GT1a increased in content, and the content of GM3 and GD3 markedly decreased. Because of these changes in composition, we determined the activities, in homogenates of embryonic brains, of two key enzymes of ganglioside synthesis: sialyltransferase for the synthesis of GD3 from GM3 and N‐acetylgalactosaminyltransferase for GM2 synthesis from GM3. The sialyltransferase activity (GM3 → GD3) was constant between E14 and E18 but decreased rapidly from E18 to birth. In contrast, the N‐acetylgalacto‐saminyltransferase activity (GM3 ± GM2) increased between E14 and E18 but was constant from E18 to birth. These changes in ganglioside composition and enzymatic activities indicate that during development there is a shift from synthesis of the simplest gangliosides of the “a” and “b” pathways to synthesis of the more complex gangliosides.

AB - Abstract: Developmental changes in ganglioside composition and biosynthesis were studied in rat brain between embryonic day (E) 14 and birth. In E14 brains, GM3 and GD3 were predominant. At E16, “b” series gangliosides, such as GD1b, GT1b, and GQ1b, increased in content. After E18, “a” series gangliosides such as GM1, GD1a, and GT1a increased in content, and the content of GM3 and GD3 markedly decreased. Because of these changes in composition, we determined the activities, in homogenates of embryonic brains, of two key enzymes of ganglioside synthesis: sialyltransferase for the synthesis of GD3 from GM3 and N‐acetylgalactosaminyltransferase for GM2 synthesis from GM3. The sialyltransferase activity (GM3 → GD3) was constant between E14 and E18 but decreased rapidly from E18 to birth. In contrast, the N‐acetylgalacto‐saminyltransferase activity (GM3 ± GM2) increased between E14 and E18 but was constant from E18 to birth. These changes in ganglioside composition and enzymatic activities indicate that during development there is a shift from synthesis of the simplest gangliosides of the “a” and “b” pathways to synthesis of the more complex gangliosides.

KW - Brain development

KW - Gangliosides

KW - N‐Acetylgalactosaminyltransferase

KW - Sialyltransferase

UR - http://www.scopus.com/inward/record.url?scp=0023951111&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023951111&partnerID=8YFLogxK

U2 - 10.1111/j.1471-4159.1988.tb02484.x

DO - 10.1111/j.1471-4159.1988.tb02484.x

M3 - Article

C2 - 3131485

AN - SCOPUS:0023951111

VL - 50

SP - 1825

EP - 1829

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 6

ER -