Developmental changes in sensitivity of bovine embryos to heat shock and use of antioxidants as thermoprotectants.

A. D. Ealy, J. L. Howell, Victor Hugo Monterroso, C. F. Aréchiga, P. J. Hansen

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Experiments were conducted with in vitro-produced bovine embryos to determine whether 1) increased culture temperatures (i.e., heat shock) adversely affected embryonic development, 2) embryos become more resistant to heat shock as they advance in development, and 3) selective antioxidant molecules alleviate heat shock effects on embryonic development. Development of 2-cell embryos to > or = 16-cell stage on d 5 after in vitro fertilization was not affected by a heat shock of 40 degrees C for 3 h, but 41 or 42 degrees C for 3 h decreased (P = .004) development. In a separate experiment, development of 2-cell embryos was decreased (P = .01) by exposure to 41 degrees C for 3 h but not for 1 h. In contrast, development of morulae to blastocysts was not affected by heat shock of 41 degrees C for 1 or 3 h. Medium supplementation with 50 nM glutathione or 50 mM taurine before heat shock did not reduce the effects of heat shock (41 degrees C for 3 h) on 2-cell embryos. Likewise, addition of glutathione ester, a more membrane-permeable analog of glutathione, did not protect 2-cell embryos from heat shock. In conclusion, early bovine embryos are susceptible to disruption in development caused by heat shock. As embryos progress in development, they acquire resistance to heat shock. Glutathione, taurine, and glutathione ester were not effective in alleviating the effects of heat shock on development of 2-cell embryos. Consequently, molecules have yet to be identified that can protect early-stage bovine embryos from the adverse effects of heat shock.

Original languageEnglish (US)
Pages (from-to)1401-1407
Number of pages7
JournalJournal of Animal Science
Volume73
Issue number5
DOIs
StatePublished - Jan 1 1995

Fingerprint

heat stress
Shock
embryo (animal)
Embryonic Structures
Antioxidants
Hot Temperature
antioxidants
cattle
Glutathione
glutathione
embryogenesis
Taurine
taurine
cells
Embryonic Development
Esters
esters
Morula
morula
in vitro fertilization

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Developmental changes in sensitivity of bovine embryos to heat shock and use of antioxidants as thermoprotectants. / Ealy, A. D.; Howell, J. L.; Monterroso, Victor Hugo; Aréchiga, C. F.; Hansen, P. J.

In: Journal of Animal Science, Vol. 73, No. 5, 01.01.1995, p. 1401-1407.

Research output: Contribution to journalArticle

Ealy, A. D. ; Howell, J. L. ; Monterroso, Victor Hugo ; Aréchiga, C. F. ; Hansen, P. J. / Developmental changes in sensitivity of bovine embryos to heat shock and use of antioxidants as thermoprotectants. In: Journal of Animal Science. 1995 ; Vol. 73, No. 5. pp. 1401-1407.
@article{260c661d90bf4f0395b30d87efe4b738,
title = "Developmental changes in sensitivity of bovine embryos to heat shock and use of antioxidants as thermoprotectants.",
abstract = "Experiments were conducted with in vitro-produced bovine embryos to determine whether 1) increased culture temperatures (i.e., heat shock) adversely affected embryonic development, 2) embryos become more resistant to heat shock as they advance in development, and 3) selective antioxidant molecules alleviate heat shock effects on embryonic development. Development of 2-cell embryos to > or = 16-cell stage on d 5 after in vitro fertilization was not affected by a heat shock of 40 degrees C for 3 h, but 41 or 42 degrees C for 3 h decreased (P = .004) development. In a separate experiment, development of 2-cell embryos was decreased (P = .01) by exposure to 41 degrees C for 3 h but not for 1 h. In contrast, development of morulae to blastocysts was not affected by heat shock of 41 degrees C for 1 or 3 h. Medium supplementation with 50 nM glutathione or 50 mM taurine before heat shock did not reduce the effects of heat shock (41 degrees C for 3 h) on 2-cell embryos. Likewise, addition of glutathione ester, a more membrane-permeable analog of glutathione, did not protect 2-cell embryos from heat shock. In conclusion, early bovine embryos are susceptible to disruption in development caused by heat shock. As embryos progress in development, they acquire resistance to heat shock. Glutathione, taurine, and glutathione ester were not effective in alleviating the effects of heat shock on development of 2-cell embryos. Consequently, molecules have yet to be identified that can protect early-stage bovine embryos from the adverse effects of heat shock.",
author = "Ealy, {A. D.} and Howell, {J. L.} and Monterroso, {Victor Hugo} and Ar{\'e}chiga, {C. F.} and Hansen, {P. J.}",
year = "1995",
month = "1",
day = "1",
doi = "10.2527/1995.7351401x",
language = "English (US)",
volume = "73",
pages = "1401--1407",
journal = "Journal of Animal Science",
issn = "0021-8812",
publisher = "American Society of Animal Science",
number = "5",

}

TY - JOUR

T1 - Developmental changes in sensitivity of bovine embryos to heat shock and use of antioxidants as thermoprotectants.

AU - Ealy, A. D.

AU - Howell, J. L.

AU - Monterroso, Victor Hugo

AU - Aréchiga, C. F.

AU - Hansen, P. J.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Experiments were conducted with in vitro-produced bovine embryos to determine whether 1) increased culture temperatures (i.e., heat shock) adversely affected embryonic development, 2) embryos become more resistant to heat shock as they advance in development, and 3) selective antioxidant molecules alleviate heat shock effects on embryonic development. Development of 2-cell embryos to > or = 16-cell stage on d 5 after in vitro fertilization was not affected by a heat shock of 40 degrees C for 3 h, but 41 or 42 degrees C for 3 h decreased (P = .004) development. In a separate experiment, development of 2-cell embryos was decreased (P = .01) by exposure to 41 degrees C for 3 h but not for 1 h. In contrast, development of morulae to blastocysts was not affected by heat shock of 41 degrees C for 1 or 3 h. Medium supplementation with 50 nM glutathione or 50 mM taurine before heat shock did not reduce the effects of heat shock (41 degrees C for 3 h) on 2-cell embryos. Likewise, addition of glutathione ester, a more membrane-permeable analog of glutathione, did not protect 2-cell embryos from heat shock. In conclusion, early bovine embryos are susceptible to disruption in development caused by heat shock. As embryos progress in development, they acquire resistance to heat shock. Glutathione, taurine, and glutathione ester were not effective in alleviating the effects of heat shock on development of 2-cell embryos. Consequently, molecules have yet to be identified that can protect early-stage bovine embryos from the adverse effects of heat shock.

AB - Experiments were conducted with in vitro-produced bovine embryos to determine whether 1) increased culture temperatures (i.e., heat shock) adversely affected embryonic development, 2) embryos become more resistant to heat shock as they advance in development, and 3) selective antioxidant molecules alleviate heat shock effects on embryonic development. Development of 2-cell embryos to > or = 16-cell stage on d 5 after in vitro fertilization was not affected by a heat shock of 40 degrees C for 3 h, but 41 or 42 degrees C for 3 h decreased (P = .004) development. In a separate experiment, development of 2-cell embryos was decreased (P = .01) by exposure to 41 degrees C for 3 h but not for 1 h. In contrast, development of morulae to blastocysts was not affected by heat shock of 41 degrees C for 1 or 3 h. Medium supplementation with 50 nM glutathione or 50 mM taurine before heat shock did not reduce the effects of heat shock (41 degrees C for 3 h) on 2-cell embryos. Likewise, addition of glutathione ester, a more membrane-permeable analog of glutathione, did not protect 2-cell embryos from heat shock. In conclusion, early bovine embryos are susceptible to disruption in development caused by heat shock. As embryos progress in development, they acquire resistance to heat shock. Glutathione, taurine, and glutathione ester were not effective in alleviating the effects of heat shock on development of 2-cell embryos. Consequently, molecules have yet to be identified that can protect early-stage bovine embryos from the adverse effects of heat shock.

UR - http://www.scopus.com/inward/record.url?scp=0029300803&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029300803&partnerID=8YFLogxK

U2 - 10.2527/1995.7351401x

DO - 10.2527/1995.7351401x

M3 - Article

VL - 73

SP - 1401

EP - 1407

JO - Journal of Animal Science

JF - Journal of Animal Science

SN - 0021-8812

IS - 5

ER -