Developmental potential of muscle cell progenitors and the myogenic factor SUM-1 in the sea urchin embryo

Judith M. Venuti, Lin Gan, Mark T. Kozlowski, William H. Klein

Research output: Contribution to journalArticle

Abstract

During sea urchin development, esophageal muscle arises from secondary mesenchyme cells, descendants of the vegetal plate that delaminate from the coelomic epithelium at the end of gastrulation. In lithium-induced exogastrulae, where vegetal plate descendants evert rather than invaginate, myogenesis occurs normally, indicating that myocyte progenitors do not have to be near the future stomodeum for differentiation to occur. Vegetal plate descendants isolated along with the extracellular matrix at different times during gastrulation produce differentiated myocytes in culture as monitored by staining with a myosin heavy chain antibody. Vegetal isolates prepared at mid-gastrulation or later consistently produce differentiated myocytes whose form and position resembled their counterparts in the intact embryo, whereas vegetal isolates prepared a few hours earlier while capable of gut differentiation, as evidenced by the de novo synthesis of the endodermal surface marker Endo 1, did not produce differentiated myocytes. These results suggest that sometime after early gastrulation, a subset of secondary mesenchyme cells are competent to differentiate into muscle cells. RNase protection assays showed that the accumulation of sea urchin myogenic factor (SUM-1) mRNA is likely to be coincident with the earliest demonstrable commitment of myogenic precursors. Premature expression of SUM-1 coding sequences in mesenchyme blastulae resulted in the activation of muscle-specific enhancer elements, demonstrating that SUM-1 can function precociously in the early embryo. However, SUM-1 expressed in this manner did not activate the endogenous MHC gene, nor induce premature or ectopic production of muscle cells.

Original languageEnglish (US)
Pages (from-to)3-14
Number of pages12
JournalMechanisms of Development
Volume41
Issue number1
DOIs
StatePublished - Apr 1993
Externally publishedYes

Fingerprint

Sea Urchins
Muscle Cells
Gastrulation
Embryonic Structures
Mesoderm
Blastula
Muscles
Myosin Heavy Chains
Muscle Development
Ribonucleases
Lithium
Extracellular Matrix
Epithelium
Staining and Labeling
Messenger RNA
Antibodies
Genes

Keywords

  • Myogenesis
  • Myogenic regulatory factor
  • Sea urchin embryo

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

Cite this

Developmental potential of muscle cell progenitors and the myogenic factor SUM-1 in the sea urchin embryo. / Venuti, Judith M.; Gan, Lin; Kozlowski, Mark T.; Klein, William H.

In: Mechanisms of Development, Vol. 41, No. 1, 04.1993, p. 3-14.

Research output: Contribution to journalArticle

Venuti, Judith M. ; Gan, Lin ; Kozlowski, Mark T. ; Klein, William H. / Developmental potential of muscle cell progenitors and the myogenic factor SUM-1 in the sea urchin embryo. In: Mechanisms of Development. 1993 ; Vol. 41, No. 1. pp. 3-14.
@article{71b6f194d84042aaa37ddd41a1c990b2,
title = "Developmental potential of muscle cell progenitors and the myogenic factor SUM-1 in the sea urchin embryo",
abstract = "During sea urchin development, esophageal muscle arises from secondary mesenchyme cells, descendants of the vegetal plate that delaminate from the coelomic epithelium at the end of gastrulation. In lithium-induced exogastrulae, where vegetal plate descendants evert rather than invaginate, myogenesis occurs normally, indicating that myocyte progenitors do not have to be near the future stomodeum for differentiation to occur. Vegetal plate descendants isolated along with the extracellular matrix at different times during gastrulation produce differentiated myocytes in culture as monitored by staining with a myosin heavy chain antibody. Vegetal isolates prepared at mid-gastrulation or later consistently produce differentiated myocytes whose form and position resembled their counterparts in the intact embryo, whereas vegetal isolates prepared a few hours earlier while capable of gut differentiation, as evidenced by the de novo synthesis of the endodermal surface marker Endo 1, did not produce differentiated myocytes. These results suggest that sometime after early gastrulation, a subset of secondary mesenchyme cells are competent to differentiate into muscle cells. RNase protection assays showed that the accumulation of sea urchin myogenic factor (SUM-1) mRNA is likely to be coincident with the earliest demonstrable commitment of myogenic precursors. Premature expression of SUM-1 coding sequences in mesenchyme blastulae resulted in the activation of muscle-specific enhancer elements, demonstrating that SUM-1 can function precociously in the early embryo. However, SUM-1 expressed in this manner did not activate the endogenous MHC gene, nor induce premature or ectopic production of muscle cells.",
keywords = "Myogenesis, Myogenic regulatory factor, Sea urchin embryo",
author = "Venuti, {Judith M.} and Lin Gan and Kozlowski, {Mark T.} and Klein, {William H.}",
year = "1993",
month = "4",
doi = "10.1016/0925-4773(93)90051-X",
language = "English (US)",
volume = "41",
pages = "3--14",
journal = "Mechanisms of Development",
issn = "0925-4773",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Developmental potential of muscle cell progenitors and the myogenic factor SUM-1 in the sea urchin embryo

AU - Venuti, Judith M.

AU - Gan, Lin

AU - Kozlowski, Mark T.

AU - Klein, William H.

PY - 1993/4

Y1 - 1993/4

N2 - During sea urchin development, esophageal muscle arises from secondary mesenchyme cells, descendants of the vegetal plate that delaminate from the coelomic epithelium at the end of gastrulation. In lithium-induced exogastrulae, where vegetal plate descendants evert rather than invaginate, myogenesis occurs normally, indicating that myocyte progenitors do not have to be near the future stomodeum for differentiation to occur. Vegetal plate descendants isolated along with the extracellular matrix at different times during gastrulation produce differentiated myocytes in culture as monitored by staining with a myosin heavy chain antibody. Vegetal isolates prepared at mid-gastrulation or later consistently produce differentiated myocytes whose form and position resembled their counterparts in the intact embryo, whereas vegetal isolates prepared a few hours earlier while capable of gut differentiation, as evidenced by the de novo synthesis of the endodermal surface marker Endo 1, did not produce differentiated myocytes. These results suggest that sometime after early gastrulation, a subset of secondary mesenchyme cells are competent to differentiate into muscle cells. RNase protection assays showed that the accumulation of sea urchin myogenic factor (SUM-1) mRNA is likely to be coincident with the earliest demonstrable commitment of myogenic precursors. Premature expression of SUM-1 coding sequences in mesenchyme blastulae resulted in the activation of muscle-specific enhancer elements, demonstrating that SUM-1 can function precociously in the early embryo. However, SUM-1 expressed in this manner did not activate the endogenous MHC gene, nor induce premature or ectopic production of muscle cells.

AB - During sea urchin development, esophageal muscle arises from secondary mesenchyme cells, descendants of the vegetal plate that delaminate from the coelomic epithelium at the end of gastrulation. In lithium-induced exogastrulae, where vegetal plate descendants evert rather than invaginate, myogenesis occurs normally, indicating that myocyte progenitors do not have to be near the future stomodeum for differentiation to occur. Vegetal plate descendants isolated along with the extracellular matrix at different times during gastrulation produce differentiated myocytes in culture as monitored by staining with a myosin heavy chain antibody. Vegetal isolates prepared at mid-gastrulation or later consistently produce differentiated myocytes whose form and position resembled their counterparts in the intact embryo, whereas vegetal isolates prepared a few hours earlier while capable of gut differentiation, as evidenced by the de novo synthesis of the endodermal surface marker Endo 1, did not produce differentiated myocytes. These results suggest that sometime after early gastrulation, a subset of secondary mesenchyme cells are competent to differentiate into muscle cells. RNase protection assays showed that the accumulation of sea urchin myogenic factor (SUM-1) mRNA is likely to be coincident with the earliest demonstrable commitment of myogenic precursors. Premature expression of SUM-1 coding sequences in mesenchyme blastulae resulted in the activation of muscle-specific enhancer elements, demonstrating that SUM-1 can function precociously in the early embryo. However, SUM-1 expressed in this manner did not activate the endogenous MHC gene, nor induce premature or ectopic production of muscle cells.

KW - Myogenesis

KW - Myogenic regulatory factor

KW - Sea urchin embryo

UR - http://www.scopus.com/inward/record.url?scp=0027196983&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027196983&partnerID=8YFLogxK

U2 - 10.1016/0925-4773(93)90051-X

DO - 10.1016/0925-4773(93)90051-X

M3 - Article

C2 - 8389581

AN - SCOPUS:0027196983

VL - 41

SP - 3

EP - 14

JO - Mechanisms of Development

JF - Mechanisms of Development

SN - 0925-4773

IS - 1

ER -