Developmental regulation and expression of the zebrafish connexin43 gene

Bishwanath Chatterjee, Alvin J. Chin, Gunnar Valdimarsson, Carla Finis, Jennifer M. Sonntag, Bo Yon Choi, Liang Tao, Krithika Balasubramanian, Carolyn Bell, Alison Krufka, David J. Kozlowski, Ross G. Johnson, Cecilia W. Lo

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We cloned and sequenced the zebrafish (Danio rerio) connexin43 (Cx43α1) gene. The predicted protein sequence shows a high degree of sequence conservation. Transcript analyses revealed multiple transcription start sites and a potential alternative transcript encoding a N-terminally truncated Cx43α1 protein. Maternal Cx43α1 transcripts were detected, with zygotic expression initiated before gastrulation. In situ hybridization revealed many Cx43α1 expression domains, including the notochord and brain, heart and vasculature, many resembling patterns seen in mammalian embryos. Of interest, a reporter construct under control of the mouse Cx43α1 promoter was observed to drive green fluorescent protein expression in zebrafish embryos in domains mimicking the native Cx43α1 expression pattern in fish and mice. Sequence comparison between the mouse and zebrafish Cx43α1 promoter sequences showed the conservation of several transcription factor motifs, which otherwise shared little overall sequence homology. The conservation of protein sequence and developmental gene regulation would suggest that Cx43α1 gap junctions are likely to have conserved roles in vertebrate embryonic development.

Original languageEnglish (US)
Pages (from-to)890-906
Number of pages17
JournalDevelopmental Dynamics
Volume233
Issue number3
DOIs
StatePublished - Jul 1 2005

Fingerprint

Connexin 43
Zebrafish
Genes
Mammalian Embryo
Developmental Genes
Notochord
Gastrulation
Proteins
Transcription Initiation Site
Gap Junctions
Sequence Homology
Green Fluorescent Proteins
Embryonic Development
In Situ Hybridization
Vertebrates
Fishes
Transcription Factors
Embryonic Structures
Mothers
Brain

Keywords

  • Connexin
  • Cx43 Cx32.2
  • Embryo
  • Gap junction
  • Mouse
  • Promoter
  • Zebrafish

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Chatterjee, B., Chin, A. J., Valdimarsson, G., Finis, C., Sonntag, J. M., Choi, B. Y., ... Lo, C. W. (2005). Developmental regulation and expression of the zebrafish connexin43 gene. Developmental Dynamics, 233(3), 890-906. https://doi.org/10.1002/dvdy.20426

Developmental regulation and expression of the zebrafish connexin43 gene. / Chatterjee, Bishwanath; Chin, Alvin J.; Valdimarsson, Gunnar; Finis, Carla; Sonntag, Jennifer M.; Choi, Bo Yon; Tao, Liang; Balasubramanian, Krithika; Bell, Carolyn; Krufka, Alison; Kozlowski, David J.; Johnson, Ross G.; Lo, Cecilia W.

In: Developmental Dynamics, Vol. 233, No. 3, 01.07.2005, p. 890-906.

Research output: Contribution to journalArticle

Chatterjee, B, Chin, AJ, Valdimarsson, G, Finis, C, Sonntag, JM, Choi, BY, Tao, L, Balasubramanian, K, Bell, C, Krufka, A, Kozlowski, DJ, Johnson, RG & Lo, CW 2005, 'Developmental regulation and expression of the zebrafish connexin43 gene', Developmental Dynamics, vol. 233, no. 3, pp. 890-906. https://doi.org/10.1002/dvdy.20426
Chatterjee B, Chin AJ, Valdimarsson G, Finis C, Sonntag JM, Choi BY et al. Developmental regulation and expression of the zebrafish connexin43 gene. Developmental Dynamics. 2005 Jul 1;233(3):890-906. https://doi.org/10.1002/dvdy.20426
Chatterjee, Bishwanath ; Chin, Alvin J. ; Valdimarsson, Gunnar ; Finis, Carla ; Sonntag, Jennifer M. ; Choi, Bo Yon ; Tao, Liang ; Balasubramanian, Krithika ; Bell, Carolyn ; Krufka, Alison ; Kozlowski, David J. ; Johnson, Ross G. ; Lo, Cecilia W. / Developmental regulation and expression of the zebrafish connexin43 gene. In: Developmental Dynamics. 2005 ; Vol. 233, No. 3. pp. 890-906.
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