Diabetes-induced peroxynitrite impairs the balance of pro-nerve growth factor and nerve growth factor, and causes neurovascular injury

T. K. Ali, M. M.H. Al-Gayyar, S. Matragoon, B. A. Pillai, M. A. Abdelsaid, Julian J Nussbaum, A. B. El-Remessy

Research output: Contribution to journalArticle

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Abstract

Aims/hypothesis: Diabetic retinopathy, the leading cause of blindness in working-age Americans, is characterised by reduced neurotrophic support and increased proinflammatory cytokines, resulting in neurotoxicity and vascular permeability. We sought to elucidate how oxidative stress impairs homeostasis of nerve growth factor (NGF) and its precursor, proform of NGF (proNGF), to cause neurovascular dysfunction in the eye of diabetic patients. Methods: Levels of NGF and proNGF were examined in samples from human patients, from retinal Müller glial cell line culture cells and from streptozotocin-induced diabetic animals treated with and without atorvastatin (10 mg/kg daily, per os) or 5,10,15,20-tetrakis (4-sulfonatophenyl) porphyrinato iron (III) chloride (FeTPPs) (15 mg/kg daily, i.p.) for 4 weeks. Neuronal death and vascular permeability were assessed by TUNEL and extravasation of BSA-fluorescein. Results: Diabetes-induced peroxynitrite formation impaired production and activity of matrix metalloproteinase-7 (MMP-7), which cleaves proNGF extracellularly, leading to accumulation of proNGF and reducing NGF in samples from diabetic retinopathy patients and experimental models. Treatment of diabetic animals with atorvastatin exerted similar protective effects that blocked peroxynitrite using FeTPPs, restoring activity of MMP-7 and hence the balance between proNGF and NGF. These effects were associated with preservation of blood-retinal barrier integrity, preventing neuronal cell death and blocking activation of RhoA and p38 mitogen-activated protein kinase (p38MAPK) in experimental and human samples. Conclusions/interpretation: Oxidative stress plays an unrecognised role in causing accumulation of proNGF, which can activate a common pathway, RhoA/p38MAPK, to mediate neurovascular injury. Oral statin therapy shows promise for treatment of diabetic retinopathy.

Original languageEnglish (US)
Pages (from-to)657-668
Number of pages12
JournalDiabetologia
Volume54
Issue number3
DOIs
StatePublished - Mar 1 2011

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Peroxynitrous Acid
Nerve Growth Factor
Wounds and Injuries
Diabetic Retinopathy
Matrix Metalloproteinase 7
Capillary Permeability
p38 Mitogen-Activated Protein Kinases
Oxidative Stress
Blood-Retinal Barrier
Hydroxymethylglutaryl-CoA Reductase Inhibitors
In Situ Nick-End Labeling
Blindness
Streptozocin
Fluorescein
Neuroglia
Homeostasis
Cell Death
Theoretical Models
Therapeutics
Cell Culture Techniques

Keywords

  • Atorvastatin
  • Diabetes
  • NGF
  • Neuroprotection
  • Peroxynitrite
  • Rho kinase
  • proNGF

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Diabetes-induced peroxynitrite impairs the balance of pro-nerve growth factor and nerve growth factor, and causes neurovascular injury. / Ali, T. K.; Al-Gayyar, M. M.H.; Matragoon, S.; Pillai, B. A.; Abdelsaid, M. A.; Nussbaum, Julian J; El-Remessy, A. B.

In: Diabetologia, Vol. 54, No. 3, 01.03.2011, p. 657-668.

Research output: Contribution to journalArticle

Ali, T. K. ; Al-Gayyar, M. M.H. ; Matragoon, S. ; Pillai, B. A. ; Abdelsaid, M. A. ; Nussbaum, Julian J ; El-Remessy, A. B. / Diabetes-induced peroxynitrite impairs the balance of pro-nerve growth factor and nerve growth factor, and causes neurovascular injury. In: Diabetologia. 2011 ; Vol. 54, No. 3. pp. 657-668.
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AU - Al-Gayyar, M. M.H.

AU - Matragoon, S.

AU - Pillai, B. A.

AU - Abdelsaid, M. A.

AU - Nussbaum, Julian J

AU - El-Remessy, A. B.

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