TY - JOUR
T1 - Diabetic ketoacidosis increases extracellular levels of the major inducible 70-kDa heat shock protein
AU - Oglesbee, Michael J.
AU - Herdman, Anne V.
AU - Passmore, Gregory G.
AU - Hoffman, William H.
N1 - Funding Information:
This work was supported by funds from the National Institute of Neurological Disorders and Stroke (R01 NS31693).
PY - 2005/10
Y1 - 2005/10
N2 - Objectives: Diabetic ketoacidosis (DKA) represents a metabolic stress whose treatment induces a systemic proinflammatory cytokine profile and accentuates life-threatening acute complications. The present study determined whether serum levels of the major inducible 70-kDa heat shock protein (Hsp72), a modulator of cytokine expression, were influenced by DKA and its treatment. Design and methods: Serum levels of Hsp72 and glucose were measured in five adolescents with type 1 diabetes mellitus (T1DM) prior to, during and following correction of severe DKA. Samples from nine relatively euglycemic T1DM patients served as controls. Results: DKA pre-treatment samples showed significant elevation in Hsp72 (40.8 ± 6.9 ng/ml) relative to euglycemic T1DM controls (33.6 ± 3.2 ng/ml) (P < 0.05). Treatment resulted in a decline in Hsp72 to control levels within 24 h, with Hsp72 and glucose levels being tightly correlated (r = 0.9258). Conclusion: Extracellular Hsp72 is increased by DKA, paralleling changes in serum glucose levels.
AB - Objectives: Diabetic ketoacidosis (DKA) represents a metabolic stress whose treatment induces a systemic proinflammatory cytokine profile and accentuates life-threatening acute complications. The present study determined whether serum levels of the major inducible 70-kDa heat shock protein (Hsp72), a modulator of cytokine expression, were influenced by DKA and its treatment. Design and methods: Serum levels of Hsp72 and glucose were measured in five adolescents with type 1 diabetes mellitus (T1DM) prior to, during and following correction of severe DKA. Samples from nine relatively euglycemic T1DM patients served as controls. Results: DKA pre-treatment samples showed significant elevation in Hsp72 (40.8 ± 6.9 ng/ml) relative to euglycemic T1DM controls (33.6 ± 3.2 ng/ml) (P < 0.05). Treatment resulted in a decline in Hsp72 to control levels within 24 h, with Hsp72 and glucose levels being tightly correlated (r = 0.9258). Conclusion: Extracellular Hsp72 is increased by DKA, paralleling changes in serum glucose levels.
KW - Blood glucose
KW - Diabetic ketoacidosis
KW - Hsp72 protein
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U2 - 10.1016/j.clinbiochem.2005.05.011
DO - 10.1016/j.clinbiochem.2005.05.011
M3 - Article
C2 - 16009359
AN - SCOPUS:24944450708
SN - 0009-9120
VL - 38
SP - 900
EP - 904
JO - Clinical Biochemistry
JF - Clinical Biochemistry
IS - 10
ER -