Dietary ca2+ increases natriuretic and diuretic responses to volume loading in nacl-sensitive spontaneously hypertensive rats

Saowalak Jirakulsomchok, Mahmood S Mozaffari, Suzanne Oparil, J. Michael Wyss

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Abstract

The present study tests the hypothesis that dietary Ca2+ supplementation increases acute diuretic and natriuretic responses to volume loading in the NaCl-sensitive spontaneously hypertensive rat (SHR-S). Seven week old male SHR-S and normotensive Wistar—Kyoto rats (WKY) were fed one of the following diets for 2.5 weeks: basal (0.75% NaCl, 0.68% Ca2 +); high NaCl (8% NaCl, 0.68% Ca2 +); high Ca2+ (0.75% NaCl, 2.0% Ca2 +); and high NaCl and Ca2+ (8% NaCl, 2.0% Ca2 +). SHR-S on high Ca2+ and on high NaCl and Ca2+ diets for 2 weeks displayed significantly lower systolic arterial pressures (SAP) than SHR-S on basal and high NaCl diets, respectively. WKY displayed no diet-related change in SAP at any time during the study. After 2.5 weeks on the diets, pre-instrumented, conscious SHR-S and WKY received an intravenous infusion (5% body weight) of isotonic NaCl, and urine was collected through a bladder catheter for a period of 90 min. The infusion did not alter the heart rate or the mean arterial pressure in any group. WKY on the high NaCl diet excreted a significantly greater percentage of the volume and Na+ load than WKY on the basal diet. In contrast, SHR-S on the high NaCl (compared to basal) diet did not display significantly enhanced natriuresis or diuresis. SHR-S on the basal diet displayed excretion rates similar to WKY on the basal diet. Dietary Ca2+ supplementation significantly increased the natriuretic and diuretic responses to saline infusion in SHR-S on the high NaCl diet, but not in SHR-S on the basal diet or in WKY on either diet. These results indicate that SHR-S on a high NaCl diet have impaired diuretic and natriuretic responses to an isotonic volume load and that dietary Ca2+ supplementation increases the ability of SHR-S on a high NaCl diet to adjust fluid volume rapidly via diuresis and natriuresis. This alteration in renal function may contribute to the hypotensive effect of a high Ca2+ diet in NaCl sensitive forms of hypertension.

Original languageEnglish (US)
Pages (from-to)947-951
Number of pages5
JournalJournal of hypertension
Volume8
Issue number10
DOIs
StatePublished - Jan 1 1990

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Inbred SHR Rats
Diuretics
Diet
Arterial Pressure
Natriuresis
Diuresis
Blood Pressure
Intravenous Infusions

Keywords

  • Diuresis
  • Hypertension
  • Kidney
  • Natriuresis
  • Salt sensitivity

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Dietary ca2+ increases natriuretic and diuretic responses to volume loading in nacl-sensitive spontaneously hypertensive rats. / Jirakulsomchok, Saowalak; Mozaffari, Mahmood S; Oparil, Suzanne; Wyss, J. Michael.

In: Journal of hypertension, Vol. 8, No. 10, 01.01.1990, p. 947-951.

Research output: Contribution to journalArticle

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AU - Wyss, J. Michael

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N2 - The present study tests the hypothesis that dietary Ca2+ supplementation increases acute diuretic and natriuretic responses to volume loading in the NaCl-sensitive spontaneously hypertensive rat (SHR-S). Seven week old male SHR-S and normotensive Wistar—Kyoto rats (WKY) were fed one of the following diets for 2.5 weeks: basal (0.75% NaCl, 0.68% Ca2 +); high NaCl (8% NaCl, 0.68% Ca2 +); high Ca2+ (0.75% NaCl, 2.0% Ca2 +); and high NaCl and Ca2+ (8% NaCl, 2.0% Ca2 +). SHR-S on high Ca2+ and on high NaCl and Ca2+ diets for 2 weeks displayed significantly lower systolic arterial pressures (SAP) than SHR-S on basal and high NaCl diets, respectively. WKY displayed no diet-related change in SAP at any time during the study. After 2.5 weeks on the diets, pre-instrumented, conscious SHR-S and WKY received an intravenous infusion (5% body weight) of isotonic NaCl, and urine was collected through a bladder catheter for a period of 90 min. The infusion did not alter the heart rate or the mean arterial pressure in any group. WKY on the high NaCl diet excreted a significantly greater percentage of the volume and Na+ load than WKY on the basal diet. In contrast, SHR-S on the high NaCl (compared to basal) diet did not display significantly enhanced natriuresis or diuresis. SHR-S on the basal diet displayed excretion rates similar to WKY on the basal diet. Dietary Ca2+ supplementation significantly increased the natriuretic and diuretic responses to saline infusion in SHR-S on the high NaCl diet, but not in SHR-S on the basal diet or in WKY on either diet. These results indicate that SHR-S on a high NaCl diet have impaired diuretic and natriuretic responses to an isotonic volume load and that dietary Ca2+ supplementation increases the ability of SHR-S on a high NaCl diet to adjust fluid volume rapidly via diuresis and natriuresis. This alteration in renal function may contribute to the hypotensive effect of a high Ca2+ diet in NaCl sensitive forms of hypertension.

AB - The present study tests the hypothesis that dietary Ca2+ supplementation increases acute diuretic and natriuretic responses to volume loading in the NaCl-sensitive spontaneously hypertensive rat (SHR-S). Seven week old male SHR-S and normotensive Wistar—Kyoto rats (WKY) were fed one of the following diets for 2.5 weeks: basal (0.75% NaCl, 0.68% Ca2 +); high NaCl (8% NaCl, 0.68% Ca2 +); high Ca2+ (0.75% NaCl, 2.0% Ca2 +); and high NaCl and Ca2+ (8% NaCl, 2.0% Ca2 +). SHR-S on high Ca2+ and on high NaCl and Ca2+ diets for 2 weeks displayed significantly lower systolic arterial pressures (SAP) than SHR-S on basal and high NaCl diets, respectively. WKY displayed no diet-related change in SAP at any time during the study. After 2.5 weeks on the diets, pre-instrumented, conscious SHR-S and WKY received an intravenous infusion (5% body weight) of isotonic NaCl, and urine was collected through a bladder catheter for a period of 90 min. The infusion did not alter the heart rate or the mean arterial pressure in any group. WKY on the high NaCl diet excreted a significantly greater percentage of the volume and Na+ load than WKY on the basal diet. In contrast, SHR-S on the high NaCl (compared to basal) diet did not display significantly enhanced natriuresis or diuresis. SHR-S on the basal diet displayed excretion rates similar to WKY on the basal diet. Dietary Ca2+ supplementation significantly increased the natriuretic and diuretic responses to saline infusion in SHR-S on the high NaCl diet, but not in SHR-S on the basal diet or in WKY on either diet. These results indicate that SHR-S on a high NaCl diet have impaired diuretic and natriuretic responses to an isotonic volume load and that dietary Ca2+ supplementation increases the ability of SHR-S on a high NaCl diet to adjust fluid volume rapidly via diuresis and natriuresis. This alteration in renal function may contribute to the hypotensive effect of a high Ca2+ diet in NaCl sensitive forms of hypertension.

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