Dietary L-arginine attenuates expression of vascular cell adhesion molecule-1 in the aortae of hypercholesterolemic rats

Yuqing Huo, Chengbin Xu, Tong Zhang, Jie Zhang, Junfeng Cui, Chunlin Yin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective To determine whether the antiatherogenic effect of L-arginine is due to an inhibition of vascular cell adhesion molecule-1 (VCAM-1) expression in the aortae of hypercholesterolemic rats. Methods Twenty-four male Wistar rats were randomized into three groups: Group NC with normal diet (NC, n=8), Group CC with 4% cholesterol and 1% cholic acid diet (CC, n=8), Group AC with 4% cholesterol and 1% cholic acid diet supplemented with 3% L-arginine HCI in the drinking water (AC, n=8). Eight weeks later, the blood samples were collected for biochemical studies, and the aortae were harvested for RT-PCR and immunohistochemical studies. Results The results showed that dietary L-arginine supplementation reduced expression of VCAM-1 in protein level and mRNA level. Conclusion Inhibitory effect of dietary supplementation of L-arginine on VCAM-1 expression may be one of the mechanisms of its antiatherosclerosis.

Original languageEnglish (US)
Pages (from-to)452-455
Number of pages4
JournalChinese Medical Journal
Volume112
Issue number5
StatePublished - May 1 1999
Externally publishedYes

Fingerprint

Vascular Cell Adhesion Molecule-1
Arginine
Aorta
Cholic Acid
Diet
Cholesterol
Dietary Supplements
Drinking Water
Wistar Rats
Polymerase Chain Reaction
Messenger RNA
Proteins

Keywords

  • Adhesion molecule
  • L-arginine
  • Vascular cell

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Dietary L-arginine attenuates expression of vascular cell adhesion molecule-1 in the aortae of hypercholesterolemic rats. / Huo, Yuqing; Xu, Chengbin; Zhang, Tong; Zhang, Jie; Cui, Junfeng; Yin, Chunlin.

In: Chinese Medical Journal, Vol. 112, No. 5, 01.05.1999, p. 452-455.

Research output: Contribution to journalArticle

Huo, Yuqing ; Xu, Chengbin ; Zhang, Tong ; Zhang, Jie ; Cui, Junfeng ; Yin, Chunlin. / Dietary L-arginine attenuates expression of vascular cell adhesion molecule-1 in the aortae of hypercholesterolemic rats. In: Chinese Medical Journal. 1999 ; Vol. 112, No. 5. pp. 452-455.
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abstract = "Objective To determine whether the antiatherogenic effect of L-arginine is due to an inhibition of vascular cell adhesion molecule-1 (VCAM-1) expression in the aortae of hypercholesterolemic rats. Methods Twenty-four male Wistar rats were randomized into three groups: Group NC with normal diet (NC, n=8), Group CC with 4{\%} cholesterol and 1{\%} cholic acid diet (CC, n=8), Group AC with 4{\%} cholesterol and 1{\%} cholic acid diet supplemented with 3{\%} L-arginine HCI in the drinking water (AC, n=8). Eight weeks later, the blood samples were collected for biochemical studies, and the aortae were harvested for RT-PCR and immunohistochemical studies. Results The results showed that dietary L-arginine supplementation reduced expression of VCAM-1 in protein level and mRNA level. Conclusion Inhibitory effect of dietary supplementation of L-arginine on VCAM-1 expression may be one of the mechanisms of its antiatherosclerosis.",
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AU - Cui, Junfeng

AU - Yin, Chunlin

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N2 - Objective To determine whether the antiatherogenic effect of L-arginine is due to an inhibition of vascular cell adhesion molecule-1 (VCAM-1) expression in the aortae of hypercholesterolemic rats. Methods Twenty-four male Wistar rats were randomized into three groups: Group NC with normal diet (NC, n=8), Group CC with 4% cholesterol and 1% cholic acid diet (CC, n=8), Group AC with 4% cholesterol and 1% cholic acid diet supplemented with 3% L-arginine HCI in the drinking water (AC, n=8). Eight weeks later, the blood samples were collected for biochemical studies, and the aortae were harvested for RT-PCR and immunohistochemical studies. Results The results showed that dietary L-arginine supplementation reduced expression of VCAM-1 in protein level and mRNA level. Conclusion Inhibitory effect of dietary supplementation of L-arginine on VCAM-1 expression may be one of the mechanisms of its antiatherosclerosis.

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