Difference in Membrane Repair Capacity Between Cancer Cell Lines and a Normal Cell Line

Stine Krog Frandsen, Anna K. McNeil, Ivana Novak, Paul L McNeil, Julie Gehl

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Electroporation-based treatments and other therapies that permeabilize the plasma membrane have been shown to be more devastating to malignant cells than to normal cells. In this study, we asked if a difference in repair capacity could explain this observed difference in sensitivity. Membrane repair was investigated by disrupting the plasma membrane using laser followed by monitoring fluorescent dye entry over time in seven cancer cell lines, an immortalized cell line, and a normal primary cell line. The kinetics of repair in living cells can be directly recorded using this technique, providing a sensitive index of repair capacity. The normal primary cell line of all tested cell lines exhibited the slowest rate of dye entry after laser disruption and lowest level of dye uptake. Significantly, more rapid dye uptake and a higher total level of dye uptake occurred in six of the seven tested cancer cell lines (p < 0.05) as well as the immortalized cell line (p < 0.001). This difference in sensitivity was also observed when a viability assay was performed one day after plasma membrane permeabilization by electroporation. Viability in the primary normal cell line (98 % viable cells) was higher than in the three tested cancer cell lines (81–88 % viable cells). These data suggest more effective membrane repair in normal, primary cells and supplement previous explanations why electroporation-based therapies and other therapies permeabilizing the plasma membrane are more effective on malignant cells compared to normal cells in cancer treatment.

Original languageEnglish (US)
Pages (from-to)569-576
Number of pages8
JournalJournal of Membrane Biology
Volume249
Issue number4
DOIs
StatePublished - Aug 1 2016

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Cell Line
Membranes
Neoplasms
Coloring Agents
Cell Membrane
Electroporation
Lasers
Electrochemotherapy
Therapeutics
Fluorescent Dyes

Keywords

  • Cancer
  • Electroporation
  • In vitro
  • Membrane repair
  • Normal

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Cell Biology

Cite this

Difference in Membrane Repair Capacity Between Cancer Cell Lines and a Normal Cell Line. / Frandsen, Stine Krog; McNeil, Anna K.; Novak, Ivana; McNeil, Paul L; Gehl, Julie.

In: Journal of Membrane Biology, Vol. 249, No. 4, 01.08.2016, p. 569-576.

Research output: Contribution to journalArticle

Frandsen, Stine Krog ; McNeil, Anna K. ; Novak, Ivana ; McNeil, Paul L ; Gehl, Julie. / Difference in Membrane Repair Capacity Between Cancer Cell Lines and a Normal Cell Line. In: Journal of Membrane Biology. 2016 ; Vol. 249, No. 4. pp. 569-576.
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