Arginine vasopressin (AVP) stimulated active Na+ transport (JNa+) and osmotic water flow JH2O) across the pelvic skin but only (JNa+) across the pectoral skin of the toad, Bufo woodhouseii. Isolated epithelial cells from the pelvic skin had a maximal c-AMP level of 11.16 pmoles/mg protein after 5 min of AVP treatment while that of pectoral skin was 3.64 pmoles/mg protein. The c-AMP level of both skin areas fell to unstimulated values after 20 min of AVP treatment; however, JH2O (pelvic skin) and (JNa+) (pelvic and pectoral skin) remained elevated during 3 hr of treatment. Dibutyryl c-AMP and theophylline stimulated JH2O across the pelvic but not the pectoral skin. Maintaining toads in water for 12-24 hr resulted in a substantial lowering of JH2O across the pectoral skin which was not reversible by treatment with c-AMP and theophylline.
|Original language||English (US)|
|Number of pages||5|
|Journal||Comparative Biochemistry and Physiology. Part C, Comparative|
|Publication status||Published - Jan 1 1985|
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