TY - JOUR
T1 - Differences in c-AMP levels in epithelial cells from pelvic and pectoral regions of the toad skin
AU - Marrero, Mario B.
AU - Hillyard, Stanley D.
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1985
Y1 - 1985
N2 - Arginine vasopressin (AVP) stimulated active Na+ transport (JNa+) and osmotic water flow JH2O) across the pelvic skin but only (JNa+) across the pectoral skin of the toad, Bufo woodhouseii. Isolated epithelial cells from the pelvic skin had a maximal c-AMP level of 11.16 pmoles/mg protein after 5 min of AVP treatment while that of pectoral skin was 3.64 pmoles/mg protein. The c-AMP level of both skin areas fell to unstimulated values after 20 min of AVP treatment; however, JH2O (pelvic skin) and (JNa+) (pelvic and pectoral skin) remained elevated during 3 hr of treatment. Dibutyryl c-AMP and theophylline stimulated JH2O across the pelvic but not the pectoral skin. Maintaining toads in water for 12-24 hr resulted in a substantial lowering of JH2O across the pectoral skin which was not reversible by treatment with c-AMP and theophylline.
AB - Arginine vasopressin (AVP) stimulated active Na+ transport (JNa+) and osmotic water flow JH2O) across the pelvic skin but only (JNa+) across the pectoral skin of the toad, Bufo woodhouseii. Isolated epithelial cells from the pelvic skin had a maximal c-AMP level of 11.16 pmoles/mg protein after 5 min of AVP treatment while that of pectoral skin was 3.64 pmoles/mg protein. The c-AMP level of both skin areas fell to unstimulated values after 20 min of AVP treatment; however, JH2O (pelvic skin) and (JNa+) (pelvic and pectoral skin) remained elevated during 3 hr of treatment. Dibutyryl c-AMP and theophylline stimulated JH2O across the pelvic but not the pectoral skin. Maintaining toads in water for 12-24 hr resulted in a substantial lowering of JH2O across the pectoral skin which was not reversible by treatment with c-AMP and theophylline.
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U2 - 10.1016/0742-8413(85)90211-7
DO - 10.1016/0742-8413(85)90211-7
M3 - Article
C2 - 2865077
AN - SCOPUS:0021817869
SN - 0306-4492
VL - 82
SP - 69
EP - 73
JO - Comparative Biochemistry and Physiology. Part C, Comparative
JF - Comparative Biochemistry and Physiology. Part C, Comparative
IS - 1
ER -