Differences in c-AMP levels in epithelial cells from pelvic and pectoral regions of the toad skin

Mario B Marrero, Stanley D. Hillyard

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Abstract

Arginine vasopressin (AVP) stimulated active Na+ transport (JNa+) and osmotic water flow JH2O) across the pelvic skin but only (JNa+) across the pectoral skin of the toad, Bufo woodhouseii. Isolated epithelial cells from the pelvic skin had a maximal c-AMP level of 11.16 pmoles/mg protein after 5 min of AVP treatment while that of pectoral skin was 3.64 pmoles/mg protein. The c-AMP level of both skin areas fell to unstimulated values after 20 min of AVP treatment; however, JH2O (pelvic skin) and (JNa+) (pelvic and pectoral skin) remained elevated during 3 hr of treatment. Dibutyryl c-AMP and theophylline stimulated JH2O across the pelvic but not the pectoral skin. Maintaining toads in water for 12-24 hr resulted in a substantial lowering of JH2O across the pectoral skin which was not reversible by treatment with c-AMP and theophylline.

Original languageEnglish (US)
Pages (from-to)69-73
Number of pages5
JournalComparative Biochemistry and Physiology. Part C, Comparative
Volume82
Issue number1
DOIs
Publication statusPublished - Jan 1 1985

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ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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