Differences in the properties of ionotropic glutamate synaptic currents in oxytocin and vasopressin neuroendocrine neurons

Javier E. Stern, Mario Galarreta, Robert C. Foehring, Shaul Hestrin, William E. Armstrong

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Oxytocin (OT) and vasopressin (VP) hormone release from neurohypophysial terminals is controlled by the firing pattern of neurosecretory cells located in the hypothalamic supraoptic (SON) and paraventricular nuclei. Although glutamate is a key modulator of the electrical activity of both OT and VP neurons, a differential contribution of AMPA receptors (AMPARs) and NMDA receptors (NMDARs) has been proposed to mediate glutamatergic influences on these neurons. In the present study we examined the distribution and functional properties of synaptic currents mediated by AMPARs and NMDARs in immunoidentified SON neurons. Our results suggest that the properties of AMPA-mediated currents in SON neurons are controlled in a cell type-specific manner. OT neurons displayed AMPA-mediated miniature EPSCs (mEPSCs) with larger amplitude and faster decay kinetics than VP neurons. Furthermore, a peak-scaled nonstationary noise analysis of mEPSCs revealed a larger estimated single-channel conductance of AMPARs expressed in OT neurons. High- frequency summation of AMPA-mediated excitatory postsynaptic potentials was smaller in OT neurons. In both cell types, AMPA-mediated synaptic currents showed inward rectification, which was more pronounced in OT neurons, and displayed Ca2+ permeability. On the other hand, NMDA-mediated mEPSCs of both cell types had similar amplitude and kinetic properties. The cell type- specific expression of functionally different AMPARs can contribute to the adoption of different firing patterns by these neuroendocrine neurons in response to physiological stimuli.

Original languageEnglish (US)
Pages (from-to)3367-3375
Number of pages9
JournalJournal of Neuroscience
Volume19
Issue number9
StatePublished - May 1 1999

Fingerprint

Oxytocin
Vasopressins
Glutamic Acid
Neurons
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
AMPA Receptors
N-Methyl-D-Aspartate Receptors
Posterior Pituitary Hormones
Supraoptic Nucleus
Paraventricular Hypothalamic Nucleus
Excitatory Postsynaptic Potentials
N-Methylaspartate
Noise
Permeability

Keywords

  • AMPA receptors
  • Hypothalamus
  • NMDA receptors
  • Oxytocin
  • Synaptic transmission
  • Vasopressin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Stern, J. E., Galarreta, M., Foehring, R. C., Hestrin, S., & Armstrong, W. E. (1999). Differences in the properties of ionotropic glutamate synaptic currents in oxytocin and vasopressin neuroendocrine neurons. Journal of Neuroscience, 19(9), 3367-3375.

Differences in the properties of ionotropic glutamate synaptic currents in oxytocin and vasopressin neuroendocrine neurons. / Stern, Javier E.; Galarreta, Mario; Foehring, Robert C.; Hestrin, Shaul; Armstrong, William E.

In: Journal of Neuroscience, Vol. 19, No. 9, 01.05.1999, p. 3367-3375.

Research output: Contribution to journalArticle

Stern, JE, Galarreta, M, Foehring, RC, Hestrin, S & Armstrong, WE 1999, 'Differences in the properties of ionotropic glutamate synaptic currents in oxytocin and vasopressin neuroendocrine neurons', Journal of Neuroscience, vol. 19, no. 9, pp. 3367-3375.
Stern, Javier E. ; Galarreta, Mario ; Foehring, Robert C. ; Hestrin, Shaul ; Armstrong, William E. / Differences in the properties of ionotropic glutamate synaptic currents in oxytocin and vasopressin neuroendocrine neurons. In: Journal of Neuroscience. 1999 ; Vol. 19, No. 9. pp. 3367-3375.
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