Differential effects of taurine treatment and taurine deficiency on the outcome of renal ischemia reperfusion injury

Mahmood S Mozaffari, Rafik A Abdelsayed, Champa Patel, Hereward Wimborne, Jun Yao Liu, Stephen W. Schaffer

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Taurine possesses membrane stabilization, osmoregulatory and antioxidant properties, aspects of relevance to ischemic injury. We tested the hypothesis that body taurine status is a determinant of renal ischemic injury. Accordingly, renal function and structure were examined in control (C), taurine-treated (TT) and taurine deficient (TD) rats that were subjected to bilateral renal ischemia (60 min) followed by reperfusion (IR); sham operated rats served as controls. Baseline urine osmolality was greater in the TD group than in the control and the TT groups, an effect associated with increased renal aquaporin 2 level. The IR insult reduced urine osmolality (i.e., day-1 post insult); the TD/IR group displayed a more marked recovery in urine osmolality by day-6 post insult than the other two groups. Fluid and sodium excretions were lower in the TD/IR group, suggesting propensity to retention. Histopathological examination revealed the presence of tubular necrotic foci in the C/IR group than sham controls. While renal architecture of the TD/IR group showed features resembling sham controls, the TT/IR group showed dilated tubules, which lacked immunostaining for aquaporin 2, but not 1, suggestive of proximal tubule origin. Finally, assessment of cell proliferation and apoptosis revealed lower proliferation but higher apoptotic foci in the TT/IR group than other IR groups. Collectively, the results indicate that body taurine status is a major determinant of renal IR injury.

Original languageEnglish (US)
Article numberS32
JournalJournal of Biomedical Science
Volume17
Issue numberSUPPL. 1
DOIs
StatePublished - Sep 1 2010

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Taurine
Reperfusion Injury
Kidney
Aquaporin 2
Osmolar Concentration
Urine
Rats
Wounds and Injuries
Control Groups
Cell proliferation
Reperfusion
Ischemia
Stabilization
Antioxidants
Sodium
Cell Proliferation
Apoptosis

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Pharmacology (medical)

Cite this

Differential effects of taurine treatment and taurine deficiency on the outcome of renal ischemia reperfusion injury. / Mozaffari, Mahmood S; Abdelsayed, Rafik A; Patel, Champa; Wimborne, Hereward; Liu, Jun Yao; Schaffer, Stephen W.

In: Journal of Biomedical Science, Vol. 17, No. SUPPL. 1, S32, 01.09.2010.

Research output: Contribution to journalArticle

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