Differential expression of integrin αv and β3 in serosal tissue of human intraperitoneal organs and adhesion

Nasser Chegini, Kristina Kotseos, Chunfeng Ma, R. Stan Williams, Michael P. Diamond, Lena Holmdahl, Keven Skinner

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objective: To assess the expression of integrin αv and β3 in the serosal tissue of intraperitoneal organs and adhesions in persons with and without adhesions. Design: Prospective study. Setting: Academic research centers. Patient(s): Fifty-seven patients undergoing abdominal or pelvic surgery. Main Outcome Measure(s): Integrin αv and β3 messenger RNA (mRNA) expression was evaluated by quantitative reverse transcription polymerase chain reaction. Result(s): The serosal tissue of the parietal peritoneum, uterus, fallopian tubes, ovary, and the large and small bowel, as well as peritoneal adhesions, skin, fascia, subcutaneous tissue, and omentum, expresses integrin αv and β3 mRNA. The level of αv and β3 mRNA expression varied among these tissues; expression of the former substance was highest in uterine serosa and lowest in the skin and small bowel, and expression of the latter substance was highest in the fallopian tubes and skin and lowest in the uterine serosa. Parietal peritoneum and adhesions express equal levels of integrin αv; however, integrin β3 expression was >100-fold lower in adhesions than in peritoneum. The level of integrin β3 expression in omentum, small and large bowels, and subcutaneous tissue was 100-fold to 10,000-fold lower than in other tissues. Conclusion(s): Serosal tissue of peritoneal organs and adhesions express variable levels of integrin αv and β3 mRNA. On the basis of such variation and the knowledge that tissue injury alters local integrin expression, integrins may play a key role in adhesion development, particularly in tissue with higher integrin expression.

Original languageEnglish (US)
Pages (from-to)791-796
Number of pages6
JournalFertility and sterility
Volume75
Issue number4
DOIs
StatePublished - Apr 19 2001

Fingerprint

Integrins
Peritoneum
Serous Membrane
Messenger RNA
Omentum
Fallopian Tubes
Subcutaneous Tissue
Skin
Fascia
Reverse Transcription
Uterus
Ovary
Outcome Assessment (Health Care)
Prospective Studies
Polymerase Chain Reaction
Wounds and Injuries
Research

Keywords

  • Adhesion
  • Human
  • Integrin expression
  • Messenger RNA
  • Peritoneum

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Differential expression of integrin αv and β3 in serosal tissue of human intraperitoneal organs and adhesion. / Chegini, Nasser; Kotseos, Kristina; Ma, Chunfeng; Williams, R. Stan; Diamond, Michael P.; Holmdahl, Lena; Skinner, Keven.

In: Fertility and sterility, Vol. 75, No. 4, 19.04.2001, p. 791-796.

Research output: Contribution to journalArticle

Chegini, Nasser ; Kotseos, Kristina ; Ma, Chunfeng ; Williams, R. Stan ; Diamond, Michael P. ; Holmdahl, Lena ; Skinner, Keven. / Differential expression of integrin αv and β3 in serosal tissue of human intraperitoneal organs and adhesion. In: Fertility and sterility. 2001 ; Vol. 75, No. 4. pp. 791-796.
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AU - Ma, Chunfeng

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AU - Diamond, Michael P.

AU - Holmdahl, Lena

AU - Skinner, Keven

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AB - Objective: To assess the expression of integrin αv and β3 in the serosal tissue of intraperitoneal organs and adhesions in persons with and without adhesions. Design: Prospective study. Setting: Academic research centers. Patient(s): Fifty-seven patients undergoing abdominal or pelvic surgery. Main Outcome Measure(s): Integrin αv and β3 messenger RNA (mRNA) expression was evaluated by quantitative reverse transcription polymerase chain reaction. Result(s): The serosal tissue of the parietal peritoneum, uterus, fallopian tubes, ovary, and the large and small bowel, as well as peritoneal adhesions, skin, fascia, subcutaneous tissue, and omentum, expresses integrin αv and β3 mRNA. The level of αv and β3 mRNA expression varied among these tissues; expression of the former substance was highest in uterine serosa and lowest in the skin and small bowel, and expression of the latter substance was highest in the fallopian tubes and skin and lowest in the uterine serosa. Parietal peritoneum and adhesions express equal levels of integrin αv; however, integrin β3 expression was >100-fold lower in adhesions than in peritoneum. The level of integrin β3 expression in omentum, small and large bowels, and subcutaneous tissue was 100-fold to 10,000-fold lower than in other tissues. Conclusion(s): Serosal tissue of peritoneal organs and adhesions express variable levels of integrin αv and β3 mRNA. On the basis of such variation and the knowledge that tissue injury alters local integrin expression, integrins may play a key role in adhesion development, particularly in tissue with higher integrin expression.

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