Differential expression of PTP1D, a protein tyrosine phosphatase with two SH2 domains, in slow and fast skeletal muscle fibers

Lin Mei, Amy M. Kachinsky, Jan E. Seiden, Ralph W. Kuncl, Jeffrey B. Miller, R. L. Huganir

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We show that PTP1D, a protein tyrosine phosphatase that contains two SH2 domains, is preferentially expressed in slow skeletal muscle fibers. Immunohistochemical staining using polyclonal antibodies against PTP1D demonstrated that PTP1D was expressed in a subpopulation of rodent muscle fibers. These fibers were identified as slow Type I fibers based on histochemical ATPase assays and slow myosin heavy chain expression. Northern and Western analyses showed that PTP1D levels were higher in predominantly slow muscles than in predominantly fast muscles. This differential expression of PTP1D in slow muscle fibers appeared by birth. In cultures of mouse myogenic cells, PTP1D was expressed after MyoD and myogenin and appeared in myotubes derived from embryonic, fetal, and postnatal myoblasts. Remarkably, PTP1D was organized into sarcomeres in a pattern coincident with myosin heavy chain, suggesting that PTP1D associates with a component of the thick filament. These results show that PTP1D is preferentially expressed in slow muscle fibers. We speculate that PTP1D may play a role in slow muscle fiber function and differentiation.

Original languageEnglish (US)
Pages (from-to)379-390
Number of pages12
JournalExperimental Cell Research
Volume224
Issue number2
DOIs
StatePublished - May 1 1996

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Protein Tyrosine Phosphatases
src Homology Domains
Skeletal Muscle Fibers
Muscles
Myosin Heavy Chains
Myogenin
Sarcomeres
Myoblasts
Adenosine Triphosphatases
Rodentia
Parturition
Staining and Labeling
Antibodies

ASJC Scopus subject areas

  • Cell Biology

Cite this

Differential expression of PTP1D, a protein tyrosine phosphatase with two SH2 domains, in slow and fast skeletal muscle fibers. / Mei, Lin; Kachinsky, Amy M.; Seiden, Jan E.; Kuncl, Ralph W.; Miller, Jeffrey B.; Huganir, R. L.

In: Experimental Cell Research, Vol. 224, No. 2, 01.05.1996, p. 379-390.

Research output: Contribution to journalArticle

Mei, Lin ; Kachinsky, Amy M. ; Seiden, Jan E. ; Kuncl, Ralph W. ; Miller, Jeffrey B. ; Huganir, R. L. / Differential expression of PTP1D, a protein tyrosine phosphatase with two SH2 domains, in slow and fast skeletal muscle fibers. In: Experimental Cell Research. 1996 ; Vol. 224, No. 2. pp. 379-390.
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