Differential expression of TGF-β1 and TGF-β3 in serosal tissues of human intraperitoneal organs and peritoneal adhesions

Nasser Chegini, Kristina Kotseos, Yong Zhao, Barbara Bennett, Frederick W. McLean, Michael Peter Diamond, Lina Holmdahl, James Burns, R. Stan Williams, Chunfeng Ma, Alpa Patel, Eugene Goldberg, Lynn Peck, Ghassen Saed, Richard Leach, Karen Collins, Frank Yealin, David Svinvich, Yoram Serokin, Peter FalkMarie Louise Ivarsson, Maria Hedgren, Maria Bergstrom, Ingrid Palmgren, Jim Burns, Kevin Skinner, Cindy Nickerson

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Elevated local expression of transforming growth factor (TGF-β) has been associated with increased incidence of peritoneal adhesion formation. In this study we determine whether differences in basal expression of TGF-β in serosal tissue of peritoneal organs correlate with incidence of adhesion formation. Serosal tissue of parietal peritoneum, uterus, oviduct, ovary, omentum, large and small bowels as well as adhesions, skin, fascia, subcutaneous tissue, peritoneal fluid and serum were collected from 57 subjects with/without adhesions who were undergoing abdominal/pelvic surgery. To determine TGF-β1 and TGF-β3 mRNA and protein expression, total RNA and protein were isolated from these tissues and along with the fluids, subjected to quantitative RT-PCR and enzyme-linked immunosorbent assay (ELISA) respectively. Tissue sections were immunostained for TGF-β1 and TGF-β3 protein. We found that TGF-β1 and TGF-β3 mRNA and protein are expressed in these tissues and present in peritoneal fluids and serum, with considerable variations in level of their expression. Comparatively, there was more variation in TGF-β1 than TGF-β3 expression without age or gender relation. Adhesions express a significantly higher TGF-β1 mRNA and have the highest TGF-β1:TGF-β3 ratio, with lowest concentrations and ratio detected in omentum, small and large bowels; in contrast uterus expresses higher TGF-β3, with lowest concentrations detected in subcutaneous tissue and large bowels (P < 0.05). A similar trend was also observed for total (active + latent) TGF-β1 protein expression, with low active TGF-β1 that was not significantly different among the tissue extracts and fluids. However, the lowest active:total TGF-β1 ratio was found in adhesions and ovary. In subjects with adhesions, the adhesions express significantly more TGF-β1 compared to parietal peritoneum (P < 0.05). Immunoreactive TGF-β1 and TGF-β3 protein were present in various cell types in these tissues with intensity reflecting their mRNA and protein expression. In conclusion, we provided evidence that serosal tissue of various peritoneal organs and adhesions express TGF-β1 and TGF-β3. Since TGF-β is expressed differently in these tissues and tissue injury often alters the expression of TGF-β, we propose that tissues with a higher basal expression of TGF-β may become predisposed to develop more adhesions compared to others.

Original languageEnglish (US)
Pages (from-to)1291-1300
Number of pages10
JournalHuman Reproduction
Volume16
Issue number6
StatePublished - Jan 1 2001
Externally publishedYes

Fingerprint

Transforming Growth Factors
Proteins
Messenger RNA
Omentum
Ascitic Fluid
Peritoneum
Subcutaneous Tissue
Uterus
Ovary

Keywords

  • Fibrotic disorder
  • Inflammatory immune response
  • Peritoneal adhesion
  • TGF-β1
  • TGF-β3

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Chegini, N., Kotseos, K., Zhao, Y., Bennett, B., McLean, F. W., Diamond, M. P., ... Nickerson, C. (2001). Differential expression of TGF-β1 and TGF-β3 in serosal tissues of human intraperitoneal organs and peritoneal adhesions. Human Reproduction, 16(6), 1291-1300.

Differential expression of TGF-β1 and TGF-β3 in serosal tissues of human intraperitoneal organs and peritoneal adhesions. / Chegini, Nasser; Kotseos, Kristina; Zhao, Yong; Bennett, Barbara; McLean, Frederick W.; Diamond, Michael Peter; Holmdahl, Lina; Burns, James; Williams, R. Stan; Ma, Chunfeng; Patel, Alpa; Goldberg, Eugene; Peck, Lynn; Saed, Ghassen; Leach, Richard; Collins, Karen; Yealin, Frank; Svinvich, David; Serokin, Yoram; Falk, Peter; Ivarsson, Marie Louise; Hedgren, Maria; Bergstrom, Maria; Palmgren, Ingrid; Burns, Jim; Skinner, Kevin; Nickerson, Cindy.

In: Human Reproduction, Vol. 16, No. 6, 01.01.2001, p. 1291-1300.

Research output: Contribution to journalArticle

Chegini, N, Kotseos, K, Zhao, Y, Bennett, B, McLean, FW, Diamond, MP, Holmdahl, L, Burns, J, Williams, RS, Ma, C, Patel, A, Goldberg, E, Peck, L, Saed, G, Leach, R, Collins, K, Yealin, F, Svinvich, D, Serokin, Y, Falk, P, Ivarsson, ML, Hedgren, M, Bergstrom, M, Palmgren, I, Burns, J, Skinner, K & Nickerson, C 2001, 'Differential expression of TGF-β1 and TGF-β3 in serosal tissues of human intraperitoneal organs and peritoneal adhesions', Human Reproduction, vol. 16, no. 6, pp. 1291-1300.
Chegini, Nasser ; Kotseos, Kristina ; Zhao, Yong ; Bennett, Barbara ; McLean, Frederick W. ; Diamond, Michael Peter ; Holmdahl, Lina ; Burns, James ; Williams, R. Stan ; Ma, Chunfeng ; Patel, Alpa ; Goldberg, Eugene ; Peck, Lynn ; Saed, Ghassen ; Leach, Richard ; Collins, Karen ; Yealin, Frank ; Svinvich, David ; Serokin, Yoram ; Falk, Peter ; Ivarsson, Marie Louise ; Hedgren, Maria ; Bergstrom, Maria ; Palmgren, Ingrid ; Burns, Jim ; Skinner, Kevin ; Nickerson, Cindy. / Differential expression of TGF-β1 and TGF-β3 in serosal tissues of human intraperitoneal organs and peritoneal adhesions. In: Human Reproduction. 2001 ; Vol. 16, No. 6. pp. 1291-1300.
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T1 - Differential expression of TGF-β1 and TGF-β3 in serosal tissues of human intraperitoneal organs and peritoneal adhesions

AU - Chegini, Nasser

AU - Kotseos, Kristina

AU - Zhao, Yong

AU - Bennett, Barbara

AU - McLean, Frederick W.

AU - Diamond, Michael Peter

AU - Holmdahl, Lina

AU - Burns, James

AU - Williams, R. Stan

AU - Ma, Chunfeng

AU - Patel, Alpa

AU - Goldberg, Eugene

AU - Peck, Lynn

AU - Saed, Ghassen

AU - Leach, Richard

AU - Collins, Karen

AU - Yealin, Frank

AU - Svinvich, David

AU - Serokin, Yoram

AU - Falk, Peter

AU - Ivarsson, Marie Louise

AU - Hedgren, Maria

AU - Bergstrom, Maria

AU - Palmgren, Ingrid

AU - Burns, Jim

AU - Skinner, Kevin

AU - Nickerson, Cindy

PY - 2001/1/1

Y1 - 2001/1/1

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AB - Elevated local expression of transforming growth factor (TGF-β) has been associated with increased incidence of peritoneal adhesion formation. In this study we determine whether differences in basal expression of TGF-β in serosal tissue of peritoneal organs correlate with incidence of adhesion formation. Serosal tissue of parietal peritoneum, uterus, oviduct, ovary, omentum, large and small bowels as well as adhesions, skin, fascia, subcutaneous tissue, peritoneal fluid and serum were collected from 57 subjects with/without adhesions who were undergoing abdominal/pelvic surgery. To determine TGF-β1 and TGF-β3 mRNA and protein expression, total RNA and protein were isolated from these tissues and along with the fluids, subjected to quantitative RT-PCR and enzyme-linked immunosorbent assay (ELISA) respectively. Tissue sections were immunostained for TGF-β1 and TGF-β3 protein. We found that TGF-β1 and TGF-β3 mRNA and protein are expressed in these tissues and present in peritoneal fluids and serum, with considerable variations in level of their expression. Comparatively, there was more variation in TGF-β1 than TGF-β3 expression without age or gender relation. Adhesions express a significantly higher TGF-β1 mRNA and have the highest TGF-β1:TGF-β3 ratio, with lowest concentrations and ratio detected in omentum, small and large bowels; in contrast uterus expresses higher TGF-β3, with lowest concentrations detected in subcutaneous tissue and large bowels (P < 0.05). A similar trend was also observed for total (active + latent) TGF-β1 protein expression, with low active TGF-β1 that was not significantly different among the tissue extracts and fluids. However, the lowest active:total TGF-β1 ratio was found in adhesions and ovary. In subjects with adhesions, the adhesions express significantly more TGF-β1 compared to parietal peritoneum (P < 0.05). Immunoreactive TGF-β1 and TGF-β3 protein were present in various cell types in these tissues with intensity reflecting their mRNA and protein expression. In conclusion, we provided evidence that serosal tissue of various peritoneal organs and adhesions express TGF-β1 and TGF-β3. Since TGF-β is expressed differently in these tissues and tissue injury often alters the expression of TGF-β, we propose that tissues with a higher basal expression of TGF-β may become predisposed to develop more adhesions compared to others.

KW - Fibrotic disorder

KW - Inflammatory immune response

KW - Peritoneal adhesion

KW - TGF-β1

KW - TGF-β3

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