Differential in vitro activity of anidulafungin, caspofungin and micafungin against Candida parapsilosis isolates recovered from a burn unit

Mahmoud A. Ghannoum, A. Chen, M. Buhari, J. Chandra, P. K. Mukherjee, D. Baxa, A. Golembieski, Jose Antonio Vazquez

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Recent studies suggest that differences in antifungal activity among echinocandins may exist. In this study, the activities of three echinocandins (anidulafungin, caspofungin, and micafungin) against Candida parapsilosis isolates from burn unit patients, healthcare workers and the hospital environment were determined. Additionally, the effect of these echinocandins on the cell morphology of caspofungin-susceptible and caspofungin-non-susceptible isolates was assessed using scanning electron microscopy (SEM). The C parapsilosis isolates obtained from patients were susceptible to anidulafungin, but were less so to caspofungin and micafungin. Isolates obtained from healthcare workers or environmental sources were susceptible to all antifungals. SEM data demonstrated that although anidulafungin and caspofungin were equally active against a caspofungin-susceptible C parapsilosis strain, they differed in their ability to damage a caspofungin-non-susceptible strain, for which lower concentrations of anidulafungin (1 mg/L) than of caspofungin (16 mg/L) were needed to induce cellular damage and distortion of the cellular morphology. To determine whether the difference in the antifungal susceptibility of C parapsilosis isolates to anidulafungin as compared to the other two echinocandins could be due to different mutations in the FKS1 gene, the sequences of the 493-bp region of this gene associated with echinocandin resistance were compared. No differences in the corresponding amino acid sequences were observed, indicating that differences in activity between anidulafungin and the other echinocandins are not related to mutations in this region. The results of this study provide evidence that differences exist between the activities of anidulafungin and the other echinocandins.

Original languageEnglish (US)
Pages (from-to)274-279
Number of pages6
JournalClinical Microbiology and Infection
Volume15
Issue number3
DOIs
StatePublished - Jan 1 2009
Externally publishedYes

Fingerprint

caspofungin
anidulafungin
Burn Units
Echinocandins
Candida
Electron Scanning Microscopy
Delivery of Health Care
Mutation
In Vitro Techniques
micafungin
Genes
Amino Acid Sequence

Keywords

  • Antifungal
  • Candida
  • Cross-resistance
  • Echinocandins
  • Fungi
  • Hot spot mutation

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Differential in vitro activity of anidulafungin, caspofungin and micafungin against Candida parapsilosis isolates recovered from a burn unit. / Ghannoum, Mahmoud A.; Chen, A.; Buhari, M.; Chandra, J.; Mukherjee, P. K.; Baxa, D.; Golembieski, A.; Vazquez, Jose Antonio.

In: Clinical Microbiology and Infection, Vol. 15, No. 3, 01.01.2009, p. 274-279.

Research output: Contribution to journalArticle

Ghannoum, Mahmoud A. ; Chen, A. ; Buhari, M. ; Chandra, J. ; Mukherjee, P. K. ; Baxa, D. ; Golembieski, A. ; Vazquez, Jose Antonio. / Differential in vitro activity of anidulafungin, caspofungin and micafungin against Candida parapsilosis isolates recovered from a burn unit. In: Clinical Microbiology and Infection. 2009 ; Vol. 15, No. 3. pp. 274-279.
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AU - Chandra, J.

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AU - Golembieski, A.

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