Differential in vivo biodistribution of 131I-labeled exosomes from diverse cellular origins and its implication for theranostic application

Mohammad H. Rashid, Thaiz F. Borin, Roxan Ara, Kartik Angara, Jingwen Cai, Bhagelu R. Achyut, Yutao Liu, Ali S. Arbab

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Exosomes are critical mediators of intercellular crosstalk and are regulator of the cellular/tumor microenvironment. Exosomes have great prospects for clinical application as a theranostic and prognostic probe. Nevertheless, the advancement of exosomes research has been thwarted by our limited knowledge of the most efficient isolation method and their in vivo trafficking. Here we have shown that a combination of two size-based methods using a 0.20 μm syringe filter and 100 k centrifuge membrane filter followed by ultracentrifugation yields a greater number of uniform exosomes. We also demonstrated the visual representation and quantification of the differential in vivo distribution of radioisotope 131I-labeled exosomes from diverse cellular origins, e.g., tumor cells with or without treatments, myeloid-derived suppressor cells and endothelial progenitor cells. We also determined that the distribution was dependent on the exosomal protein/cytokine contents. The applied in vivo imaging modalities can be utilized to monitor disease progression, metastasis, and exosome-based targeted therapy.

Original languageEnglish (US)
Article number102072
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume21
DOIs
StatePublished - Oct 2019

Keywords

  • EPC exosomes
  • Exosomes
  • Isolation
  • MDSC exosomes
  • Radioisotope labeling
  • in vivo imaging

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

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