The influence of PGF2∝ on c-jun gene expression in ovary, uterus and adrenal was examined. Three and seven day postovulatory PMSG primed immature rats received 500 μg PGF2∝ by two subcutaneous injections 8 hours apart. Control rats received saline. Animals were sacrified 30 minutes after the second injection of PGF2∝. Tissues were obtained and frozen in liquid nitrogen. RNA extracted from ovary, uterus and adrenal was analyzed by Northern and slot blot. c-jun was expressed differentially in these organs. An increase in c-jun expression by PGF2∝ treatment occurred in the ovary but not in the adrenal and uterus. The effect of PGF2∝ on c-jun was stronger in older compared to younger corpora lutea. These results indicate differential regulation of c-jun by PGF2∝ in steroidogenic and steroid responsive tissues and that c-jun might be linked to the mode of action of PGF2∝ in luteolysis.
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