Differential induction of NAD(P)H:Quinone oxidoreductase by anti-carcinogenic organosulfides from garlic

Shivendra V. Singh, Su Shu Pan, Sanjay K. Srivastava, Hong Xia, Xun Hu, Howard A. Zaren, John L. Orchard

Research output: Contribution to journalArticle

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Abstract

This study was undertaken to elucidate the mechanism of organ specificity and differential efficacy of garlic organosulfides (OSCs) [diallyl sulfide (DAS), diallyl disulfide (DADS), diallyl trisulfide (DATS), dipropyl sulfide (DPS) and dipropyl disulfide (DPDS)] in preventing benzo(a)pyrene (BP)-induced tumorigenesis in mice. The results of the present study reveal a good correlation between chemopreventive efficacies of garlic OSCs and their inductive effects on the expression of NAD(P)H:quinone oxidoreductase (NQO), an enzyme implicated in the detoxification of activated quinone metabolites of BP. Treatment of mice with DADS and DATS, which are potent inhibitors of BP-induced forestomach tumorigenesis, resulted in a statistically significant increase (2.4- and 1.5-fold, respectively) in forestomach NQO activity. In addition, DADS and DATS were much more potent inducers of forestomach NQO activity than DAS, which is a weak inhibitor of BP-induced forestomach tumorigenesis than the former compounds. Propyl-group containing OSCs (DPS and DPDS), which do not inhibit BP-induced tumorigenesis, did not affect forestomach NQO activity. Similar to forestomach, a good correlation was also observed between effects of these OSCs against BP-induced pulmonary tumorigenesis and their effects on NQO expression in the lung. For example, treatment of mice with DAS, which is a potent inhibitor of BP-induced pulmonary tumorigenesis, resulted in about 3.2-fold increase in pulmonary NQO activity. On the other hand, this activity was increased by about 1.5-fold upon DATS administration, which does not inhibit BP-induced cancer of the lung. In conclusion, our results suggest that induction of NQO may be important in anti-cancer effects of garlic OSCs.

Original languageEnglish (US)
Pages (from-to)917-920
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume244
Issue number3
DOIs
StatePublished - Mar 27 1998

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Garlic
NAD
Oxidoreductases
Carcinogenesis
Lung
Detoxification
Benzo(a)pyrene
Metabolites
Organ Specificity
benzoquinone
diallyl trisulfide
Lung Neoplasms
Enzymes
allyl sulfide
diallyl disulfide
n-propyl disulfide
dipropyl sulfide
Neoplasms

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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Differential induction of NAD(P)H:Quinone oxidoreductase by anti-carcinogenic organosulfides from garlic. / Singh, Shivendra V.; Pan, Su Shu; Srivastava, Sanjay K.; Xia, Hong; Hu, Xun; Zaren, Howard A.; Orchard, John L.

In: Biochemical and Biophysical Research Communications, Vol. 244, No. 3, 27.03.1998, p. 917-920.

Research output: Contribution to journalArticle

Singh, Shivendra V. ; Pan, Su Shu ; Srivastava, Sanjay K. ; Xia, Hong ; Hu, Xun ; Zaren, Howard A. ; Orchard, John L. / Differential induction of NAD(P)H:Quinone oxidoreductase by anti-carcinogenic organosulfides from garlic. In: Biochemical and Biophysical Research Communications. 1998 ; Vol. 244, No. 3. pp. 917-920.
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AU - Orchard, John L.

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