Differential mechanisms of adenosine- and ATPγS-induced microvascular endothelial barrier strengthening

Róbert Bátori, Sanjiv Kumar, Zsuzsanna Bordán, Mary Cherian-Shaw, Anita Kovács-Kása, Justin A. MacDonald, David J Fulton, Ferenc Erdődi, Alexander Dmitriyevich Verin

Research output: Contribution to journalArticle

Abstract

Maintenance of the endothelial cell (EC) barrier is critical to vascular homeostasis and a loss of barrier integrity results in increased vascular permeability. While the mechanisms that govern increased EC permeability have been under intense investigation over the past several decades, the processes regulating the preservation/restoration of the EC barrier remain poorly understood. Herein we show that the extracellular purines, adenosine (Ado) and adenosine 5′-[γ-thio]-triphosphate (ATPγS) can strengthen the barrier function of human lung microvascular EC (HLMVEC). This ability involves protein kinase A (PKA) activation and decreases in myosin light chain 20 (MLC20) phosphorylation secondary to the involvement of MLC phosphatase (MLCP). In contrast to Ado, ATPγS-induced PKA activation is accompanied by a modest, but significant decrease in cyclic adenosine monophosphate (cAMP) levels supporting the existence of an unconventional cAMP-independent pathway of PKA activation. Furthermore, ATPγS-induced EC barrier strengthening does not involve the Rap guanine nucleotide exchange factor 3 (EPAC1) which is directly activated by cAMP but is instead dependent upon PKA-anchor protein 2 (AKAP2) expression. We also found that AKAP2 can directly interact with the myosin phosphatase-targeting protein MYPT1 and that depletion of AKAP2 abolished ATPγS-induced increases in transendothelial electrical resistance. Ado-induced strengthening of the HLMVEC barrier required the coordinated activation of PKA and EPAC1 in a cAMP-dependent manner. In summary, ATPγS-induced enhancement of the EC barrier is EPAC1-independent and is instead mediated by activation of PKA which is then guided by AKAP2, in a cAMP-independent mechanism, to activate MLCP which dephosphorylates MLC20 resulting in reduced EC contraction and preservation.

Original languageEnglish (US)
Pages (from-to)5863-5879
Number of pages17
JournalJournal of Cellular Physiology
Volume234
Issue number5
DOIs
StatePublished - May 1 2019

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Endothelial cells
Adenosine
Endothelial Cells
Cyclic AMP-Dependent Protein Kinases
Cyclic AMP
Chemical activation
Myosin Light Chains
Phosphoric Monoester Hydrolases
A Kinase Anchor Proteins
Myosin-Light-Chain Phosphatase
Guanine Nucleotide Exchange Factors
Lung
Purines
Phosphorylation
Acoustic impedance
Capillary Permeability
Protein Transport
Electric Impedance
Restoration
Blood Vessels

Keywords

  • ATPγS
  • PKA
  • adenosine
  • endothelial barrier protection
  • myosin light chain

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Differential mechanisms of adenosine- and ATPγS-induced microvascular endothelial barrier strengthening. / Bátori, Róbert; Kumar, Sanjiv; Bordán, Zsuzsanna; Cherian-Shaw, Mary; Kovács-Kása, Anita; MacDonald, Justin A.; Fulton, David J; Erdődi, Ferenc; Verin, Alexander Dmitriyevich.

In: Journal of Cellular Physiology, Vol. 234, No. 5, 01.05.2019, p. 5863-5879.

Research output: Contribution to journalArticle

Bátori, Róbert ; Kumar, Sanjiv ; Bordán, Zsuzsanna ; Cherian-Shaw, Mary ; Kovács-Kása, Anita ; MacDonald, Justin A. ; Fulton, David J ; Erdődi, Ferenc ; Verin, Alexander Dmitriyevich. / Differential mechanisms of adenosine- and ATPγS-induced microvascular endothelial barrier strengthening. In: Journal of Cellular Physiology. 2019 ; Vol. 234, No. 5. pp. 5863-5879.
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AU - Kovács-Kása, Anita

AU - MacDonald, Justin A.

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AU - Erdődi, Ferenc

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