Differentially expressed genes in PPARγ-deficient MSCs

Yun Su, Xiaona Shen, Jie Chen, Carlos M Isales, Jing Zhao, Xing Ming Shi

Research output: Contribution to journalReview article

3 Scopus citations

Abstract

Peroxisome proliferator-activated receptor gamma (PPARγ) is a key regulator of adipogenesis. It is also a central player in energy metabolism, inflammation and immunity. As an important nuclear transcription factor, PPARγ can regulate the expression and function of genes or biological processes directly or indirectly via association with other factors and thus modulate their activities. To better understand the impact of PPARγ on the global gene expression profile, we evaluated the bioinformatic data, which revealed the changes that occurred in genes and their pathways in the absence of PPARγ. In brief, we performed RNA deep sequencing (RNA-Seq) analysis using RNA samples isolated from multipotent mesenchymal stromal cells (MSCs) of PPARγ knockout and wild type control mice. The RNA-Seq data sets were then subjected to bioinformatic analyses from various angles to better reveal the breadth of PPARγ function in different biological processes. Our results reveal novel genes and networks modulated by PPARγ and provides new insights into our understanding of the physiologic and pathophysiologic role this nuclear receptor plays in health and disease.

Original languageEnglish (US)
Pages (from-to)97-104
Number of pages8
JournalMolecular and Cellular Endocrinology
Volume471
DOIs
Publication statusPublished - Aug 15 2018

    Fingerprint

Keywords

  • Adipocyte
  • Bone marrow
  • Differential expression
  • Inflammation
  • MSC
  • PPARγ
  • RNA-Seq

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this