Differentiated bronchiolar epithelium in alveolar ducts of rats exposed to ozone for 20 months

K. E. Pinkerton, D. E. Dodge, J. Cederdahl-Demmler, V. J. Wong, J. Peake, C. J. Haselton, P. W. Mellick, G. Singh, C. G. Plopper

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The effects of exposure to 1.0 ppm of ozone for twenty months were studied in male Fischer 344 rats. Light microscopic, morphometric, and immunohistological approaches were used to determine the distribution and degree of differentiation of ciliated and nonciliated bronchiolar epithelial (Clara) cells lining alveolar ducts of the central acinus, a primary target of ozone-induced lung injury. Alveolar duct pathways extending beyond the level of the most proximal alveolar outpocketing of terminal bronchioles were isolated in longitudinal profile. The distance that ciliated and nonciliated bronchiolar epithelial (Clara) cells projected down each alveolar duct pathway was determined by placing concentric arcs radiating outward from a single reference point at the level of the first alveolar outpocketing. A high degree of heterogeneity in the magnitude of bronchiolar epithelial cell extension into alveolar ducts was noted for each isolation and animal. Age- matched control animals also demonstrated variation in the degree of bronchiolar epithelial cell extension down alveolar ducts. In animals exposed to ozone, a striking similarity was noted by scanning electron microscopy in the surface characteristics of cells living both terminal bronchioles and alveolar ducts. The presence of Clara cell secretory protein in cells of bronchioles and alveolar ducts was also detected immunohistochemically and visualized using confocal laser scanning microscopy to the reflectance mode. Well-differentiated ciliated and nonciliated bronchiolar epithelial cells were found lining alveolar septal tips and alveoli up to a depth of 1,000 μ into the pulmonary acinus after 20 months of exposure to ozone. No evidence of inflammation was present in alveolar ducts, suggesting that epithelial cell transformations in alveolar ducts is a natural consequence of lifetime exposures to oxidant gases.

Original languageEnglish (US)
Pages (from-to)947-956
Number of pages10
JournalAmerican Journal of Pathology
Volume142
Issue number3
StatePublished - Dec 1 1993
Externally publishedYes

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Ozone
Epithelium
Epithelial Cells
Bronchioles
Uteroglobin
Alveolar Epithelial Cells
Inbred F344 Rats
Lung Injury
Oxidants
Confocal Microscopy
Electron Scanning Microscopy
Gases
Inflammation
Light
Lung

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Pinkerton, K. E., Dodge, D. E., Cederdahl-Demmler, J., Wong, V. J., Peake, J., Haselton, C. J., ... Plopper, C. G. (1993). Differentiated bronchiolar epithelium in alveolar ducts of rats exposed to ozone for 20 months. American Journal of Pathology, 142(3), 947-956.

Differentiated bronchiolar epithelium in alveolar ducts of rats exposed to ozone for 20 months. / Pinkerton, K. E.; Dodge, D. E.; Cederdahl-Demmler, J.; Wong, V. J.; Peake, J.; Haselton, C. J.; Mellick, P. W.; Singh, G.; Plopper, C. G.

In: American Journal of Pathology, Vol. 142, No. 3, 01.12.1993, p. 947-956.

Research output: Contribution to journalArticle

Pinkerton, KE, Dodge, DE, Cederdahl-Demmler, J, Wong, VJ, Peake, J, Haselton, CJ, Mellick, PW, Singh, G & Plopper, CG 1993, 'Differentiated bronchiolar epithelium in alveolar ducts of rats exposed to ozone for 20 months', American Journal of Pathology, vol. 142, no. 3, pp. 947-956.
Pinkerton KE, Dodge DE, Cederdahl-Demmler J, Wong VJ, Peake J, Haselton CJ et al. Differentiated bronchiolar epithelium in alveolar ducts of rats exposed to ozone for 20 months. American Journal of Pathology. 1993 Dec 1;142(3):947-956.
Pinkerton, K. E. ; Dodge, D. E. ; Cederdahl-Demmler, J. ; Wong, V. J. ; Peake, J. ; Haselton, C. J. ; Mellick, P. W. ; Singh, G. ; Plopper, C. G. / Differentiated bronchiolar epithelium in alveolar ducts of rats exposed to ozone for 20 months. In: American Journal of Pathology. 1993 ; Vol. 142, No. 3. pp. 947-956.
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AU - Wong, V. J.

AU - Peake, J.

AU - Haselton, C. J.

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