Differentiating asthma phenotypes in young adults through polyclonal cytokine profiles

Edward Zoratti, Suzanne Havstad, Ganesa Wegienka, Charlotte Nicholas, Kevin R. Bobbitt, Kimberley J. Woodcroft, Dennis Randall Ownby, Christine Cole Johnson

Research output: Contribution to journalArticle

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Abstract

Background Recent research has emphasized the need to better discriminate asthma phenotypes and consider underlying mechanistic endotypes in epidemiologic and clinical studies. Although allergic asthma and nonallergic asthma are frequently combined into 1 disease category in observational research and clinical trials, few studies have investigated the extent to which these 2 separate phenotypes are associated with distinct cytokine immunologic profiles in a representative young adult population. Objective To investigate the cytokine production-based endotypes underlying the clinical phenotypes of allergic and nonallergic asthma in a population-based birth cohort evaluated as young adults. Methods Participants included 18- to 21-year-old members (n = 540) of a suburban Detroit birth cohort study, the Childhood Allergy Study. Phorbol myristate acetate-stimulated whole blood interleukin (IL)-4, IL-5, IL-10, IL-12, IL-13, IL-17A, IL-17F, IL-22, and interferon-γ secretory responses were analyzed for associations comparing participants with allergic vs nonallergic asthma phenotypes with those without asthma. Results T-helper cell type (T H) 2-polarized responses, measured as higher mean IL-5 and IL-13 secretions and lower ratios of interferon-γ and IL-12 to 3 TH2 cytokines (IL-4, IL-5, or IL-13), were observed only in participants with allergic asthma. Nonallergic asthma was associated with TH1-polarized responses, including higher adjusted interferon-γ secretion compared with participants with allergic asthma and, surprisingly, those without asthma (odds ratio 2.5, confidence interval 1.2-5.1, P <.01). Conclusion As expected, young adults with a history of an allergic asthma phenotype exhibited a T H2-polarized cytokine response after polyclonal stimulation. However, TH1 polarization was observed in patients with a history of nonallergic asthma. Allergic and nonallergic asthma are associated with etiologically distinct immune endotypes, underscoring the importance of discriminating these endotypes in research analyses and clinical management.

Original languageEnglish (US)
Pages (from-to)25-30
Number of pages6
JournalAnnals of Allergy, Asthma and Immunology
Volume113
Issue number1
DOIs
StatePublished - Jan 1 2014

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Young Adult
Asthma
Cytokines
Phenotype
Interleukin-13
Interleukin-5
Interferons
Interleukin-17
Interleukin-12
Helper-Inducer T-Lymphocytes
Interleukin-4
Research
Parturition
Tetradecanoylphorbol Acetate
Interleukin-10
Population
Epidemiologic Studies
Hypersensitivity
Cohort Studies
Odds Ratio

ASJC Scopus subject areas

  • Immunology and Allergy
  • Pulmonary and Respiratory Medicine

Cite this

Zoratti, E., Havstad, S., Wegienka, G., Nicholas, C., Bobbitt, K. R., Woodcroft, K. J., ... Johnson, C. C. (2014). Differentiating asthma phenotypes in young adults through polyclonal cytokine profiles. Annals of Allergy, Asthma and Immunology, 113(1), 25-30. https://doi.org/10.1016/j.anai.2014.04.013

Differentiating asthma phenotypes in young adults through polyclonal cytokine profiles. / Zoratti, Edward; Havstad, Suzanne; Wegienka, Ganesa; Nicholas, Charlotte; Bobbitt, Kevin R.; Woodcroft, Kimberley J.; Ownby, Dennis Randall; Johnson, Christine Cole.

In: Annals of Allergy, Asthma and Immunology, Vol. 113, No. 1, 01.01.2014, p. 25-30.

Research output: Contribution to journalArticle

Zoratti, E, Havstad, S, Wegienka, G, Nicholas, C, Bobbitt, KR, Woodcroft, KJ, Ownby, DR & Johnson, CC 2014, 'Differentiating asthma phenotypes in young adults through polyclonal cytokine profiles', Annals of Allergy, Asthma and Immunology, vol. 113, no. 1, pp. 25-30. https://doi.org/10.1016/j.anai.2014.04.013
Zoratti, Edward ; Havstad, Suzanne ; Wegienka, Ganesa ; Nicholas, Charlotte ; Bobbitt, Kevin R. ; Woodcroft, Kimberley J. ; Ownby, Dennis Randall ; Johnson, Christine Cole. / Differentiating asthma phenotypes in young adults through polyclonal cytokine profiles. In: Annals of Allergy, Asthma and Immunology. 2014 ; Vol. 113, No. 1. pp. 25-30.
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