Direct electrochemical evaluation of plasma membrane cholesterol in live mammalian cells

Dechen Jiang, Anando Devadoss, M. Simona Palencsár, Danjun Fang, Nicole M. White, Thomas J. Kelley, Jonathan D. Smith, James Burgess

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Cholesterol is a tightly regulated structural component of the cell plasma membrane. Dysfunctional intracellular cholesterol transport machinery causes disease states with altered plasma membrane cholesterol. This Communication reports microelectrode evaluation of plasma membrane cholesterol of single cells at physiological temperature. Electrochemical data indicate that transport of intracellular cholesterol to the plasma membrane is active in an atherosclerotic macrophage model.

Original languageEnglish (US)
Pages (from-to)11352-11353
Number of pages2
JournalJournal of the American Chemical Society
Volume129
Issue number37
DOIs
StatePublished - Sep 19 2007

Fingerprint

Cholesterol
Cell membranes
Cells
Cell Membrane
Macrophages
Microelectrodes
Machinery
Temperature
Communication

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Jiang, D., Devadoss, A., Palencsár, M. S., Fang, D., White, N. M., Kelley, T. J., ... Burgess, J. (2007). Direct electrochemical evaluation of plasma membrane cholesterol in live mammalian cells. Journal of the American Chemical Society, 129(37), 11352-11353. https://doi.org/10.1021/ja074373c

Direct electrochemical evaluation of plasma membrane cholesterol in live mammalian cells. / Jiang, Dechen; Devadoss, Anando; Palencsár, M. Simona; Fang, Danjun; White, Nicole M.; Kelley, Thomas J.; Smith, Jonathan D.; Burgess, James.

In: Journal of the American Chemical Society, Vol. 129, No. 37, 19.09.2007, p. 11352-11353.

Research output: Contribution to journalArticle

Jiang, D, Devadoss, A, Palencsár, MS, Fang, D, White, NM, Kelley, TJ, Smith, JD & Burgess, J 2007, 'Direct electrochemical evaluation of plasma membrane cholesterol in live mammalian cells', Journal of the American Chemical Society, vol. 129, no. 37, pp. 11352-11353. https://doi.org/10.1021/ja074373c
Jiang D, Devadoss A, Palencsár MS, Fang D, White NM, Kelley TJ et al. Direct electrochemical evaluation of plasma membrane cholesterol in live mammalian cells. Journal of the American Chemical Society. 2007 Sep 19;129(37):11352-11353. https://doi.org/10.1021/ja074373c
Jiang, Dechen ; Devadoss, Anando ; Palencsár, M. Simona ; Fang, Danjun ; White, Nicole M. ; Kelley, Thomas J. ; Smith, Jonathan D. ; Burgess, James. / Direct electrochemical evaluation of plasma membrane cholesterol in live mammalian cells. In: Journal of the American Chemical Society. 2007 ; Vol. 129, No. 37. pp. 11352-11353.
@article{bbd8bbc35e694c8cb3a063a3bc71ab0a,
title = "Direct electrochemical evaluation of plasma membrane cholesterol in live mammalian cells",
abstract = "Cholesterol is a tightly regulated structural component of the cell plasma membrane. Dysfunctional intracellular cholesterol transport machinery causes disease states with altered plasma membrane cholesterol. This Communication reports microelectrode evaluation of plasma membrane cholesterol of single cells at physiological temperature. Electrochemical data indicate that transport of intracellular cholesterol to the plasma membrane is active in an atherosclerotic macrophage model.",
author = "Dechen Jiang and Anando Devadoss and Palencs{\'a}r, {M. Simona} and Danjun Fang and White, {Nicole M.} and Kelley, {Thomas J.} and Smith, {Jonathan D.} and James Burgess",
year = "2007",
month = "9",
day = "19",
doi = "10.1021/ja074373c",
language = "English (US)",
volume = "129",
pages = "11352--11353",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "37",

}

TY - JOUR

T1 - Direct electrochemical evaluation of plasma membrane cholesterol in live mammalian cells

AU - Jiang, Dechen

AU - Devadoss, Anando

AU - Palencsár, M. Simona

AU - Fang, Danjun

AU - White, Nicole M.

AU - Kelley, Thomas J.

AU - Smith, Jonathan D.

AU - Burgess, James

PY - 2007/9/19

Y1 - 2007/9/19

N2 - Cholesterol is a tightly regulated structural component of the cell plasma membrane. Dysfunctional intracellular cholesterol transport machinery causes disease states with altered plasma membrane cholesterol. This Communication reports microelectrode evaluation of plasma membrane cholesterol of single cells at physiological temperature. Electrochemical data indicate that transport of intracellular cholesterol to the plasma membrane is active in an atherosclerotic macrophage model.

AB - Cholesterol is a tightly regulated structural component of the cell plasma membrane. Dysfunctional intracellular cholesterol transport machinery causes disease states with altered plasma membrane cholesterol. This Communication reports microelectrode evaluation of plasma membrane cholesterol of single cells at physiological temperature. Electrochemical data indicate that transport of intracellular cholesterol to the plasma membrane is active in an atherosclerotic macrophage model.

UR - http://www.scopus.com/inward/record.url?scp=35048859977&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35048859977&partnerID=8YFLogxK

U2 - 10.1021/ja074373c

DO - 10.1021/ja074373c

M3 - Article

C2 - 17718573

AN - SCOPUS:35048859977

VL - 129

SP - 11352

EP - 11353

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 37

ER -