Direct inhibition of basolateral Kir4.1/5.1 and Kir4.1 channels in the cortical collecting duct by dopamine

Oleg L. Zaika, Mykola Mamenko, Oleg Palygin, Nabila Boukelmoune, Alexander Staruschenko, Oleh Pochynyuk

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

It is recognized that dopamine promotes natriuresis by inhibiting multiple transporting systems in the proximal tubule. In contrast, less is known about the molecular targets of dopamine actions on water-electrolyte transport in the cortical collecting duct (CCD). Epithelial cells in the CCD are exposed to dopamine, which is synthesized locally or secreted from sympathetic nerve endings. Basolateral K+ channels in the distal renal tubule are critical for K+ recycling and controlling basolateral membrane potential to establish the driving force for Na+ reabsorption. Here, we demonstrate that Kir4.1 and Kir5.1 are highly expressed in the mouse kidney cortex and are localized to the basolateral membrane of the CCD. Using patch-clamp electrophysiology in freshly isolated CCDs, we detected highly abundant 40-pS and scarce 20-pS single channel conductances, most likely representing Kir4.1/5.1 and Kir4.1 channels, respectively. Dopamine reversibly decreased the open probability of both channels, with a relatively greater action on the Kir4.1/5.1 heterodimer. This effect was mediated by D2-like but not D1-like dopamine receptors. PKC blockade abolished the inhibition of basolateral K+ channels by dopamine. Importantly, dopamine significantly decreased the amplitude of Kir4.1/5.1 and Kir4.1 unitary currents. Consistently, dopamine induced an acute depolarization of basolateral membrane potential, as directly monitored using currentclamp mode in isolated CCDs. Therefore, we demonstrate that dopamine inhibits basolateral Kir4.1/5.1 and Kir4.1 channels in CCD cells via stimulation of D2-like receptors and subsequently PKC. This leads to depolarization of the basolateral membrane and a decreased driving force for Na+ reabsorption in the distal renal tubule.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume305
Issue number9
DOIs
StatePublished - Nov 1 2013
Externally publishedYes

Fingerprint

Dopamine
Distal Kidney Tubule
Membrane Potentials
Kidney Cortex
Dopamine D1 Receptors
Natriuresis
Membranes
Nerve Endings
Electrophysiology
Recycling
Electrolytes
Epithelial Cells
Water

Keywords

  • Basolateral potassium recycling
  • Distal nephron
  • Dopamine receptors
  • Renal potassium channels

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

Direct inhibition of basolateral Kir4.1/5.1 and Kir4.1 channels in the cortical collecting duct by dopamine. / Zaika, Oleg L.; Mamenko, Mykola; Palygin, Oleg; Boukelmoune, Nabila; Staruschenko, Alexander; Pochynyuk, Oleh.

In: American Journal of Physiology - Renal Physiology, Vol. 305, No. 9, 01.11.2013.

Research output: Contribution to journalArticle

Zaika, Oleg L. ; Mamenko, Mykola ; Palygin, Oleg ; Boukelmoune, Nabila ; Staruschenko, Alexander ; Pochynyuk, Oleh. / Direct inhibition of basolateral Kir4.1/5.1 and Kir4.1 channels in the cortical collecting duct by dopamine. In: American Journal of Physiology - Renal Physiology. 2013 ; Vol. 305, No. 9.
@article{782974cf7bf8460597c4e18b40445091,
title = "Direct inhibition of basolateral Kir4.1/5.1 and Kir4.1 channels in the cortical collecting duct by dopamine",
abstract = "It is recognized that dopamine promotes natriuresis by inhibiting multiple transporting systems in the proximal tubule. In contrast, less is known about the molecular targets of dopamine actions on water-electrolyte transport in the cortical collecting duct (CCD). Epithelial cells in the CCD are exposed to dopamine, which is synthesized locally or secreted from sympathetic nerve endings. Basolateral K+ channels in the distal renal tubule are critical for K+ recycling and controlling basolateral membrane potential to establish the driving force for Na+ reabsorption. Here, we demonstrate that Kir4.1 and Kir5.1 are highly expressed in the mouse kidney cortex and are localized to the basolateral membrane of the CCD. Using patch-clamp electrophysiology in freshly isolated CCDs, we detected highly abundant 40-pS and scarce 20-pS single channel conductances, most likely representing Kir4.1/5.1 and Kir4.1 channels, respectively. Dopamine reversibly decreased the open probability of both channels, with a relatively greater action on the Kir4.1/5.1 heterodimer. This effect was mediated by D2-like but not D1-like dopamine receptors. PKC blockade abolished the inhibition of basolateral K+ channels by dopamine. Importantly, dopamine significantly decreased the amplitude of Kir4.1/5.1 and Kir4.1 unitary currents. Consistently, dopamine induced an acute depolarization of basolateral membrane potential, as directly monitored using currentclamp mode in isolated CCDs. Therefore, we demonstrate that dopamine inhibits basolateral Kir4.1/5.1 and Kir4.1 channels in CCD cells via stimulation of D2-like receptors and subsequently PKC. This leads to depolarization of the basolateral membrane and a decreased driving force for Na+ reabsorption in the distal renal tubule.",
keywords = "Basolateral potassium recycling, Distal nephron, Dopamine receptors, Renal potassium channels",
author = "Zaika, {Oleg L.} and Mykola Mamenko and Oleg Palygin and Nabila Boukelmoune and Alexander Staruschenko and Oleh Pochynyuk",
year = "2013",
month = "11",
day = "1",
doi = "10.1152/ajprenal.00363.2013",
language = "English (US)",
volume = "305",
journal = "American Journal of Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "9",

}

TY - JOUR

T1 - Direct inhibition of basolateral Kir4.1/5.1 and Kir4.1 channels in the cortical collecting duct by dopamine

AU - Zaika, Oleg L.

AU - Mamenko, Mykola

AU - Palygin, Oleg

AU - Boukelmoune, Nabila

AU - Staruschenko, Alexander

AU - Pochynyuk, Oleh

PY - 2013/11/1

Y1 - 2013/11/1

N2 - It is recognized that dopamine promotes natriuresis by inhibiting multiple transporting systems in the proximal tubule. In contrast, less is known about the molecular targets of dopamine actions on water-electrolyte transport in the cortical collecting duct (CCD). Epithelial cells in the CCD are exposed to dopamine, which is synthesized locally or secreted from sympathetic nerve endings. Basolateral K+ channels in the distal renal tubule are critical for K+ recycling and controlling basolateral membrane potential to establish the driving force for Na+ reabsorption. Here, we demonstrate that Kir4.1 and Kir5.1 are highly expressed in the mouse kidney cortex and are localized to the basolateral membrane of the CCD. Using patch-clamp electrophysiology in freshly isolated CCDs, we detected highly abundant 40-pS and scarce 20-pS single channel conductances, most likely representing Kir4.1/5.1 and Kir4.1 channels, respectively. Dopamine reversibly decreased the open probability of both channels, with a relatively greater action on the Kir4.1/5.1 heterodimer. This effect was mediated by D2-like but not D1-like dopamine receptors. PKC blockade abolished the inhibition of basolateral K+ channels by dopamine. Importantly, dopamine significantly decreased the amplitude of Kir4.1/5.1 and Kir4.1 unitary currents. Consistently, dopamine induced an acute depolarization of basolateral membrane potential, as directly monitored using currentclamp mode in isolated CCDs. Therefore, we demonstrate that dopamine inhibits basolateral Kir4.1/5.1 and Kir4.1 channels in CCD cells via stimulation of D2-like receptors and subsequently PKC. This leads to depolarization of the basolateral membrane and a decreased driving force for Na+ reabsorption in the distal renal tubule.

AB - It is recognized that dopamine promotes natriuresis by inhibiting multiple transporting systems in the proximal tubule. In contrast, less is known about the molecular targets of dopamine actions on water-electrolyte transport in the cortical collecting duct (CCD). Epithelial cells in the CCD are exposed to dopamine, which is synthesized locally or secreted from sympathetic nerve endings. Basolateral K+ channels in the distal renal tubule are critical for K+ recycling and controlling basolateral membrane potential to establish the driving force for Na+ reabsorption. Here, we demonstrate that Kir4.1 and Kir5.1 are highly expressed in the mouse kidney cortex and are localized to the basolateral membrane of the CCD. Using patch-clamp electrophysiology in freshly isolated CCDs, we detected highly abundant 40-pS and scarce 20-pS single channel conductances, most likely representing Kir4.1/5.1 and Kir4.1 channels, respectively. Dopamine reversibly decreased the open probability of both channels, with a relatively greater action on the Kir4.1/5.1 heterodimer. This effect was mediated by D2-like but not D1-like dopamine receptors. PKC blockade abolished the inhibition of basolateral K+ channels by dopamine. Importantly, dopamine significantly decreased the amplitude of Kir4.1/5.1 and Kir4.1 unitary currents. Consistently, dopamine induced an acute depolarization of basolateral membrane potential, as directly monitored using currentclamp mode in isolated CCDs. Therefore, we demonstrate that dopamine inhibits basolateral Kir4.1/5.1 and Kir4.1 channels in CCD cells via stimulation of D2-like receptors and subsequently PKC. This leads to depolarization of the basolateral membrane and a decreased driving force for Na+ reabsorption in the distal renal tubule.

KW - Basolateral potassium recycling

KW - Distal nephron

KW - Dopamine receptors

KW - Renal potassium channels

UR - http://www.scopus.com/inward/record.url?scp=84887009013&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84887009013&partnerID=8YFLogxK

U2 - 10.1152/ajprenal.00363.2013

DO - 10.1152/ajprenal.00363.2013

M3 - Article

VL - 305

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 1931-857X

IS - 9

ER -