Direct-repeat analysis of chromatid interactions during intrachromosomal recombination in mouse cells

Roni J. Bollag, R. Michael Liskay

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Homologous intrachromosomal recombination between linked genes can involve interactions that are either intramolecular (intrachromatid) or intermolecular (sister chromatid). To assess the relative proportions of chromatid interactions, we report studies of intrachromosomal recombination in mouse L cells containing herpes simplex virus thymidine kinase genes in two alternative configurations of direct repeats. By comparing products of reciprocal exchanges between these two configurations, we conclude that the majority of interactions that give rise to crossover products involve unequally paired sister chromatids after DNA replication. Analyses of an additional class of crossover products that involve discontinuous associated gene conversion suggest that these recombination events involve a heteroduplex DNA intermediate.

Original languageEnglish (US)
Pages (from-to)4839-4845
Number of pages7
JournalMolecular and Cellular Biology
Volume11
Issue number10
DOIs
StatePublished - 1991

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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