Discovering G protein-coupled receptor motifs mediating export from the endoplasmic reticulum

Chunmin Dong; Guangyu Wu

doi:10.1007/978-1-61779-179-6_8

Discovering G protein-coupled receptor motifs mediating export from the endoplasmic reticulum

Chunmin Dong, Guangyu Wu

Pharmacology and Toxicology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Similar to many other plasma membrane proteins, G protein-coupled receptors (GPCRs) are synthesized in the endoplasmic reticulum (ER). After proper assembly and folding, the receptors are transported from the ER through the Golgi to the cell surface. As the first step in the anterograde trafficking, exit from the ER of nascent GPCRs plays a crucial role in receptor biosynthesis and controls receptor expression at the cell surface, which in turn dictates the functionality of the receptors. Recent studies have revealed that GPCRs possess linear motifs that are required for their export from the ER. In this chapter, we will discuss experimental approaches to identify the GPCR motifs which regulate anterograde transport, specifically exit from the ER, by focusing on α _2B-adrenergic receptor.

Original language	English (US)
Title of host publication	Methods for the Discovery and Characterization of G Protein-Coupled Receptors
Editors	Craig W. Stevens
Pages	153-166
Number of pages	14
DOIs	https://doi.org/10.1007/978-1-61779-179-6_8
State	Published - 2011

Publication series

Name	Neuromethods
Volume	60
ISSN (Print)	0893-2336
ISSN (Electronic)	1940-6045

Keywords

Anterograde transport
Cell surface expression
ER
Export
GPCR
GPCR trafficking
Ligand binding
Motif

ASJC Scopus subject areas

Psychiatry and Mental health
General Pharmacology, Toxicology and Pharmaceutics
General Biochemistry, Genetics and Molecular Biology
General Neuroscience

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-61779-179-6_8

Cite this

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KW - Export

KW - GPCR

KW - GPCR trafficking

KW - Ligand binding

KW - Motif

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Discovering G protein-coupled receptor motifs mediating export from the endoplasmic reticulum

Abstract

Publication series

Keywords

ASJC Scopus subject areas

UN SDGs

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