Discovering G protein-coupled receptor motifs mediating export from the endoplasmic reticulum

Chunmin Dong, Guangyu Wu

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Similar to many other plasma membrane proteins, G protein-coupled receptors (GPCRs) are synthesized in the endoplasmic reticulum (ER). After proper assembly and folding, the receptors are transported from the ER through the Golgi to the cell surface. As the first step in the anterograde trafficking, exit from the ER of nascent GPCRs plays a crucial role in receptor biosynthesis and controls receptor expression at the cell surface, which in turn dictates the functionality of the receptors. Recent studies have revealed that GPCRs possess linear motifs that are required for their export from the ER. In this chapter, we will discuss experimental approaches to identify the GPCR motifs which regulate anterograde transport, specifically exit from the ER, by focusing on α 2B-adrenergic receptor.

Original languageEnglish (US)
Title of host publicationMethods for the Discovery and Characterization of G Protein-Coupled Receptors
EditorsCraig W. Stevens
Pages153-166
Number of pages14
DOIs
StatePublished - Aug 4 2011

Publication series

NameNeuromethods
Volume60
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Keywords

  • Anterograde transport
  • Cell surface expression
  • ER
  • Export
  • GPCR
  • GPCR trafficking
  • Ligand binding
  • Motif

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Neuroscience(all)
  • Psychiatry and Mental health

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  • Cite this

    Dong, C., & Wu, G. (2011). Discovering G protein-coupled receptor motifs mediating export from the endoplasmic reticulum. In C. W. Stevens (Ed.), Methods for the Discovery and Characterization of G Protein-Coupled Receptors (pp. 153-166). (Neuromethods; Vol. 60). https://doi.org/10.1007/978-1-61779-179-6_8