Abstract
Urinary tract infection (UTI) is a widespread infection and affects millions of people around the globe. The gold standard for identification of microorganisms causing infection is urine culture. However, current methods require at least 24 h for the results. In clinical settings, identification and discrimination of bacteria with less time-consuming and cheaper methods are highly desired. In recent years, the power of surface-enhanced Raman scattering (SERS) for fast identification of bacteria and biomolecules has been demon-strated. In this study, we show discrimination of urinary tract infection causative pathogens within 1 h of incubation using principal component analysis (PCA) of SERS spectra of seven different UTI causative bacterial species. In addition, we showed differentiation of them at their different growth phases. We also analyzed origins of bacterial SERS spectra and demonstrated the highly dynamic structure of the bacteria cell wall during their growth.
Original language | English (US) |
---|---|
Pages (from-to) | 8233-8241 |
Number of pages | 9 |
Journal | Analytical and bioanalytical chemistry |
Volume | 407 |
Issue number | 27 |
DOIs | |
State | Published - Nov 2015 |
Externally published | Yes |
Keywords
- Bacteria
- Growth phase
- Principal component analysis
- Surface-enhanced Raman scattering
- Urinary tract infection
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry