Disruption-induced mucus secretion

Repair and protection

K. Miyake, T. Tanaka, P. L. McNeil

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

When a cell suffers a plasma membrane disruption, extracellular Ca 2+ rapidly diffuses into its cytosol, triggering there local homotypic and exocytotic membrane fusion events. One role of this emergency exocytotic response is to promote cell survival: the internal membrane thus added to the plasma membrane acts as a reparative "patch." Another, unexplored consequence of disruption-induced exocytosis is secretion. Many of the cells lining the gastrointestinal tract secrete mucus via a compound exocytotic mechanism, and these and other epithelial cell types lining the digestive tract are normally subject to plasma membrane disruption injury in vivo. Here we show that plasma membrane disruption triggers a potent mucus secretory response from stomach mucous cells wounded in vitro by shear stress or by laser irradiation. This disruption-induced secretory response is Ca 2+ dependent, and coupled to cell resealing: disruption in the absence of Ca2+ does not trigger mucus release, but results instead in cell death due to failure to reseal. Ca2+-dependent, disruption-induced mucus secretion and resealing were also demonstrable in segments of intact rat large intestine. We propose that, in addition to promoting cell survival of membrane disruptions, disruption-induced exocytosis serves also the important protective function of liberating lubricating mucus at sites of mechanical wear and tear. This mode of mechanotransduction can, we propose, explain how lubrication in the gastrointestinal tract is rapidly and precisely adjusted to widely fluctuating, diet-dependent levels of mechanical stress.

Original languageEnglish (US)
Pages (from-to)1525-1531
Number of pages7
JournalPLoS Biology
Volume4
Issue number9
DOIs
StatePublished - Sep 19 2006

Fingerprint

Mucus
Cell membranes
mucus
Repair
Cell Membrane
secretion
plasma membrane
Gastrointestinal Tract
Membranes
Linings
exocytosis
Exocytosis
Cells
gastrointestinal system
cell viability
Cell Survival
cells
Lubrication
Cell death
Laser beam effects

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Disruption-induced mucus secretion : Repair and protection. / Miyake, K.; Tanaka, T.; McNeil, P. L.

In: PLoS Biology, Vol. 4, No. 9, 19.09.2006, p. 1525-1531.

Research output: Contribution to journalArticle

Miyake, K, Tanaka, T & McNeil, PL 2006, 'Disruption-induced mucus secretion: Repair and protection', PLoS Biology, vol. 4, no. 9, pp. 1525-1531. https://doi.org/10.1371/journal.pbio.0040276
Miyake, K. ; Tanaka, T. ; McNeil, P. L. / Disruption-induced mucus secretion : Repair and protection. In: PLoS Biology. 2006 ; Vol. 4, No. 9. pp. 1525-1531.
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