Disruption of adenosine 2A receptor exacerbates NAFLD through increasing inflammatory responses and SREBP1c activity

Yuli Cai, Honggui Li, Mengyang Liu, Ya Pei, Juan Zheng, Jing Zhou, Xianjun Luo, Wenya Huang, Linqiang Ma, Qiuhua Yang, Shaodong Guo, Xiaoqiu Xiao, Qifu Li, Tianshu Zeng, Fanyin Meng, Heather Francis, Shannon Glaser, Lulu Chen, Yuqing Huo, Gianfranco AlpiniChaodong Wu

Research output: Contribution to journalArticle

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Abstract

Adenosine 2A receptor (A2AR) exerts protective roles in endotoxin- and/or ischemia-induced tissue damage. However, the role for A2AR in nonalcoholic fatty liver disease (NAFLD) remains largely unknown. We sought to examine the effects of global and/or myeloid cell-specific A2AR disruption on the aspects of obesity-associated NAFLD and to elucidate the underlying mechanisms. Global and/or myeloid cell–specific A2AR-disrupted mice and control mice were fed a high-fat diet (HFD) to induce NAFLD. In addition, bone marrow–derived macrophages and primary mouse hepatocytes were examined for inflammatory and metabolic responses. Upon feeding an HFD, both global A2AR-disrupted mice and myeloid cell–specific A2AR-defcient mice revealed increased severity of HFD-induced hepatic steatosis and inflammation compared with their respective control mice. In in vitro experiments, A2AR-deficient macrophages exhibited increased proinflammatory responses, and enhanced fat deposition of wild-type primary hepatocytes in macrophage–hepatocyte cocultures. In primary hepatocytes, A2AR deficiency increased the proinflammatory responses and enhanced the effect of palmitate on stimulating fat deposition. Moreover, A2AR deficiency significantly increased the abundance of sterol regulatory element-binding protein 1c (SREBP1c) in livers of fasted mice and in hepatocytes upon nutrient deprivation. In the absence of A2AR, SREBP1c transcription activity was significantly increased in mouse hepatocytes. Conclusion: Taken together, our results demonstrate that disruption of A2AR in both macrophage and hepatocytes accounts for increased severity of NAFLD, likely through increasing inflammation and through elevating lipogenic events due to stimulation of SREBP1c expression and transcription activity. (Hepatology 2018;68:48-61).

Original languageEnglish (US)
Pages (from-to)48-61
Number of pages14
JournalHepatology
Volume68
Issue number1
DOIs
StatePublished - Jul 2018

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Sterol Regulatory Element Binding Protein 1
Purinergic P1 Receptors
Hepatocytes
High Fat Diet
Macrophages
Non-alcoholic Fatty Liver Disease
Fats
Inflammation
Palmitates
Liver
Gastroenterology
Myeloid Cells
Coculture Techniques
Endotoxins
Ischemia
Obesity

ASJC Scopus subject areas

  • Hepatology

Cite this

Disruption of adenosine 2A receptor exacerbates NAFLD through increasing inflammatory responses and SREBP1c activity. / Cai, Yuli; Li, Honggui; Liu, Mengyang; Pei, Ya; Zheng, Juan; Zhou, Jing; Luo, Xianjun; Huang, Wenya; Ma, Linqiang; Yang, Qiuhua; Guo, Shaodong; Xiao, Xiaoqiu; Li, Qifu; Zeng, Tianshu; Meng, Fanyin; Francis, Heather; Glaser, Shannon; Chen, Lulu; Huo, Yuqing; Alpini, Gianfranco; Wu, Chaodong.

In: Hepatology, Vol. 68, No. 1, 07.2018, p. 48-61.

Research output: Contribution to journalArticle

Cai, Y, Li, H, Liu, M, Pei, Y, Zheng, J, Zhou, J, Luo, X, Huang, W, Ma, L, Yang, Q, Guo, S, Xiao, X, Li, Q, Zeng, T, Meng, F, Francis, H, Glaser, S, Chen, L, Huo, Y, Alpini, G & Wu, C 2018, 'Disruption of adenosine 2A receptor exacerbates NAFLD through increasing inflammatory responses and SREBP1c activity', Hepatology, vol. 68, no. 1, pp. 48-61. https://doi.org/10.1002/hep.29777
Cai, Yuli ; Li, Honggui ; Liu, Mengyang ; Pei, Ya ; Zheng, Juan ; Zhou, Jing ; Luo, Xianjun ; Huang, Wenya ; Ma, Linqiang ; Yang, Qiuhua ; Guo, Shaodong ; Xiao, Xiaoqiu ; Li, Qifu ; Zeng, Tianshu ; Meng, Fanyin ; Francis, Heather ; Glaser, Shannon ; Chen, Lulu ; Huo, Yuqing ; Alpini, Gianfranco ; Wu, Chaodong. / Disruption of adenosine 2A receptor exacerbates NAFLD through increasing inflammatory responses and SREBP1c activity. In: Hepatology. 2018 ; Vol. 68, No. 1. pp. 48-61.
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abstract = "Adenosine 2A receptor (A2AR) exerts protective roles in endotoxin- and/or ischemia-induced tissue damage. However, the role for A2AR in nonalcoholic fatty liver disease (NAFLD) remains largely unknown. We sought to examine the effects of global and/or myeloid cell-specific A2AR disruption on the aspects of obesity-associated NAFLD and to elucidate the underlying mechanisms. Global and/or myeloid cell–specific A2AR-disrupted mice and control mice were fed a high-fat diet (HFD) to induce NAFLD. In addition, bone marrow–derived macrophages and primary mouse hepatocytes were examined for inflammatory and metabolic responses. Upon feeding an HFD, both global A2AR-disrupted mice and myeloid cell–specific A2AR-defcient mice revealed increased severity of HFD-induced hepatic steatosis and inflammation compared with their respective control mice. In in vitro experiments, A2AR-deficient macrophages exhibited increased proinflammatory responses, and enhanced fat deposition of wild-type primary hepatocytes in macrophage–hepatocyte cocultures. In primary hepatocytes, A2AR deficiency increased the proinflammatory responses and enhanced the effect of palmitate on stimulating fat deposition. Moreover, A2AR deficiency significantly increased the abundance of sterol regulatory element-binding protein 1c (SREBP1c) in livers of fasted mice and in hepatocytes upon nutrient deprivation. In the absence of A2AR, SREBP1c transcription activity was significantly increased in mouse hepatocytes. Conclusion: Taken together, our results demonstrate that disruption of A2AR in both macrophage and hepatocytes accounts for increased severity of NAFLD, likely through increasing inflammation and through elevating lipogenic events due to stimulation of SREBP1c expression and transcription activity. (Hepatology 2018;68:48-61).",
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AU - Cai, Yuli

AU - Li, Honggui

AU - Liu, Mengyang

AU - Pei, Ya

AU - Zheng, Juan

AU - Zhou, Jing

AU - Luo, Xianjun

AU - Huang, Wenya

AU - Ma, Linqiang

AU - Yang, Qiuhua

AU - Guo, Shaodong

AU - Xiao, Xiaoqiu

AU - Li, Qifu

AU - Zeng, Tianshu

AU - Meng, Fanyin

AU - Francis, Heather

AU - Glaser, Shannon

AU - Chen, Lulu

AU - Huo, Yuqing

AU - Alpini, Gianfranco

AU - Wu, Chaodong

PY - 2018/7

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N2 - Adenosine 2A receptor (A2AR) exerts protective roles in endotoxin- and/or ischemia-induced tissue damage. However, the role for A2AR in nonalcoholic fatty liver disease (NAFLD) remains largely unknown. We sought to examine the effects of global and/or myeloid cell-specific A2AR disruption on the aspects of obesity-associated NAFLD and to elucidate the underlying mechanisms. Global and/or myeloid cell–specific A2AR-disrupted mice and control mice were fed a high-fat diet (HFD) to induce NAFLD. In addition, bone marrow–derived macrophages and primary mouse hepatocytes were examined for inflammatory and metabolic responses. Upon feeding an HFD, both global A2AR-disrupted mice and myeloid cell–specific A2AR-defcient mice revealed increased severity of HFD-induced hepatic steatosis and inflammation compared with their respective control mice. In in vitro experiments, A2AR-deficient macrophages exhibited increased proinflammatory responses, and enhanced fat deposition of wild-type primary hepatocytes in macrophage–hepatocyte cocultures. In primary hepatocytes, A2AR deficiency increased the proinflammatory responses and enhanced the effect of palmitate on stimulating fat deposition. Moreover, A2AR deficiency significantly increased the abundance of sterol regulatory element-binding protein 1c (SREBP1c) in livers of fasted mice and in hepatocytes upon nutrient deprivation. In the absence of A2AR, SREBP1c transcription activity was significantly increased in mouse hepatocytes. Conclusion: Taken together, our results demonstrate that disruption of A2AR in both macrophage and hepatocytes accounts for increased severity of NAFLD, likely through increasing inflammation and through elevating lipogenic events due to stimulation of SREBP1c expression and transcription activity. (Hepatology 2018;68:48-61).

AB - Adenosine 2A receptor (A2AR) exerts protective roles in endotoxin- and/or ischemia-induced tissue damage. However, the role for A2AR in nonalcoholic fatty liver disease (NAFLD) remains largely unknown. We sought to examine the effects of global and/or myeloid cell-specific A2AR disruption on the aspects of obesity-associated NAFLD and to elucidate the underlying mechanisms. Global and/or myeloid cell–specific A2AR-disrupted mice and control mice were fed a high-fat diet (HFD) to induce NAFLD. In addition, bone marrow–derived macrophages and primary mouse hepatocytes were examined for inflammatory and metabolic responses. Upon feeding an HFD, both global A2AR-disrupted mice and myeloid cell–specific A2AR-defcient mice revealed increased severity of HFD-induced hepatic steatosis and inflammation compared with their respective control mice. In in vitro experiments, A2AR-deficient macrophages exhibited increased proinflammatory responses, and enhanced fat deposition of wild-type primary hepatocytes in macrophage–hepatocyte cocultures. In primary hepatocytes, A2AR deficiency increased the proinflammatory responses and enhanced the effect of palmitate on stimulating fat deposition. Moreover, A2AR deficiency significantly increased the abundance of sterol regulatory element-binding protein 1c (SREBP1c) in livers of fasted mice and in hepatocytes upon nutrient deprivation. In the absence of A2AR, SREBP1c transcription activity was significantly increased in mouse hepatocytes. Conclusion: Taken together, our results demonstrate that disruption of A2AR in both macrophage and hepatocytes accounts for increased severity of NAFLD, likely through increasing inflammation and through elevating lipogenic events due to stimulation of SREBP1c expression and transcription activity. (Hepatology 2018;68:48-61).

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