Distinct effects of different phosphatidylglycerol species on mouse keratinocyte proliferation

Ding Xie, Mutsa Seremwe, John G. Edwards, Robert Podolsky, Wendy B Bollag

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We have previously shown that liposomes composed of egg-derived phosphatidylglycerol (PG), with a mixed fatty acid composition (comprising mainly palmitate and oleate), inhibit the proliferation and promote the differentiation of rapidly dividing keratinocytes, and stimulate the growth of slowly proliferating epidermal cells. To determine the species of PG most effective at modulating keratinocyte proliferation, primary mouse keratinocytes were treated with different PG species, and proliferation was measured. PG species containing polyunsaturated fatty acids were effective at inhibiting rapidly proliferating keratinocytes, whereas PG species with monounsaturated fatty acids were effective at promoting proliferation in slowly dividing cells. Thus, palmitoyl-arachidonyl-PG (16:0/20:4), palmitoyl-linoleoyl-PG (16:0/18:2), dilinoleoyl-PG (18:2/18:2) and soy PG (a PG mixture with a large percentage of polyunsaturated fatty acids) were particularly effective at inhibiting proliferation in rapidly dividing keratinocytes. Conversely, palmitoyl-oleoyl-PG (16:0/18:1) and dioleoyl-PG (18:1/18:1) were especially effective proproliferative PG species. This result represents the first demonstration of opposite effects of different species of a single class of phospholipid and suggests that these different PG species may signal to diverse effector enzymes to differentially affect keratinocyte proliferation and normalize keratinocyte proliferation. Thus, different PG species may be useful for treating skin diseases characterized by excessive or insufficient proliferation.

Original languageEnglish (US)
Article numbere107119
JournalPLoS One
Volume9
Issue number9
DOIs
StatePublished - Sep 18 2014

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Phosphatidylglycerols
phosphatidylglycerols
keratinocytes
Keratinocytes
mice
Unsaturated Fatty Acids
polyunsaturated fatty acids
Monounsaturated Fatty Acids
Palmitates
palmitates
skin diseases
Oleic Acid
monounsaturated fatty acids
Skin Diseases
Liposomes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Distinct effects of different phosphatidylglycerol species on mouse keratinocyte proliferation. / Xie, Ding; Seremwe, Mutsa; Edwards, John G.; Podolsky, Robert; Bollag, Wendy B.

In: PLoS One, Vol. 9, No. 9, e107119, 18.09.2014.

Research output: Contribution to journalArticle

Xie, Ding ; Seremwe, Mutsa ; Edwards, John G. ; Podolsky, Robert ; Bollag, Wendy B. / Distinct effects of different phosphatidylglycerol species on mouse keratinocyte proliferation. In: PLoS One. 2014 ; Vol. 9, No. 9.
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