Distinct roles of neuronal and microglial CB2 cannabinoid receptors in the mouse hippocampus

Yong Li, Jimok Kim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The effects of cannabinoids are primarily mediated by type-1 cannabinoid receptors in the brain and type-2 cannabinoid receptors (CB2Rs) in the peripheral immune system. However, recent evidence demonstrates that CB2Rs are also expressed in the brain and implicated in neuropsychiatric effects. Diverse types of cells in various regions in the brain express CB2Rs but the cellular loci of CB2Rs that induce specific behavioral effects have not been determined. To manipulate CB2R expression in specific types of cells in the dorsal hippocampus of adult mice, we used Cre-dependent overexpression and CRISPR-Cas9 genome-editing techniques in combination with adeno-associated viruses and transgenic mice. Elevation and disruption of CB2R expression in microglia in the CA1 area increased and decreased, respectively, contextual fear memory. In CA1 pyramidal neurons, disruption of CB2R expression enhanced spatial working memory, whereas their overexpression reduced anxiety levels assessed as an increase in the exploration time in the central area of open field. Interneuronal CB2Rs were not involved in the modulation of cognitive or emotional behaviors tested in this study. The targeted manipulation of CB2R expression in pyramidal neurons and microglia suggests that CB2Rs in different types of cells in the mature hippocampus play distinct roles in the regulation of memory and anxiety.

Original languageEnglish (US)
Pages (from-to)11-25
Number of pages15
JournalNeuroscience
Volume363
DOIs
StatePublished - Nov 5 2017

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Hippocampus
Pyramidal Cells
Microglia
Clustered Regularly Interspaced Short Palindromic Repeats
Anxiety
Cannabinoid Receptors
Dependovirus
Cannabinoids
Brain
Short-Term Memory
Transgenic Mice
Fear
Immune System
Mouse Cnr2 protein
Gene Editing
rat Cnr1 protein
Spatial Memory

Keywords

  • Cnr2 gene
  • adeno-associated virus
  • behavior
  • memory
  • microglia
  • pyramidal neuron

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Distinct roles of neuronal and microglial CB2 cannabinoid receptors in the mouse hippocampus. / Li, Yong; Kim, Jimok.

In: Neuroscience, Vol. 363, 05.11.2017, p. 11-25.

Research output: Contribution to journalArticle

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